NKX6.1 transcription factor: a crucial regulator of pancreatic β cell development, identity, and proliferation
<p dir="ltr">Understanding the biology underlying the mechanisms and pathways regulating pancreatic β cell development is necessary to understand the pathology of diabetes mellitus (DM), which is characterized by the progressive reduction in insulin-producing β cell mass. Pluripotent...
محفوظ في:
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| مؤلفون آخرون: | |
| منشور في: |
2020
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| الموضوعات: | |
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إضافة وسم
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| _version_ | 1864513512273870848 |
|---|---|
| author | Idil I. Aigha (14913774) |
| author2 | Essam M. Abdelalim (5768072) |
| author2_role | author |
| author_facet | Idil I. Aigha (14913774) Essam M. Abdelalim (5768072) |
| author_role | author |
| dc.creator.none.fl_str_mv | Idil I. Aigha (14913774) Essam M. Abdelalim (5768072) |
| dc.date.none.fl_str_mv | 2020-10-01T00:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1186/s13287-020-01977-0 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/NKX6_1_transcription_factor_a_crucial_regulator_of_pancreatic_cell_development_identity_and_proliferation/26114467 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Clinical sciences Medical biotechnology Diabetes Transcription factor β cell mass Pluripotent stem cells Pancreatic progenitors Cell therapy |
| dc.title.none.fl_str_mv | NKX6.1 transcription factor: a crucial regulator of pancreatic β cell development, identity, and proliferation |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Understanding the biology underlying the mechanisms and pathways regulating pancreatic β cell development is necessary to understand the pathology of diabetes mellitus (DM), which is characterized by the progressive reduction in insulin-producing β cell mass. Pluripotent stem cells (PSCs) can potentially offer an unlimited supply of functional β cells for cellular therapy and disease modeling of DM. Homeobox protein NKX6.1 is a transcription factor (TF) that plays a critical role in pancreatic β cell function and proliferation. In human pancreatic islet, NKX6.1 expression is exclusive to β cells and is undetectable in other islet cells. Several reports showed that activation of NKX6.1 in PSC-derived pancreatic progenitors (MPCs), expressing PDX1 (PDX1+/NKX6.1+), warrants their future commitment to monohormonal β cells. However, further differentiation of MPCs lacking NKX6.1 expression (PDX1+/NKX6.1−) results in an undesirable generation of non-functional polyhormonal β cells. The importance of NKX6.1 as a crucial regulator in MPC specification into functional β cells directs attentions to further investigating its mechanism and enhancing NKX6.1 expression as a means to increase β cell function and mass. Here, we shed light on the role of NKX6.1 during pancreatic β cell development and in directing the MPCs to functional monohormonal lineage. Furthermore, we address the transcriptional mechanisms and targets of NKX6.1 as well as its association with diabetes.</p><h2>Other Information</h2><p dir="ltr">Published in: Stem Cell Research & Therapy<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1186/s13287-020-01977-0" target="_blank">https://dx.doi.org/10.1186/s13287-020-01977-0</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_8c5f10d32dd75de350f0397b13ff513a |
| identifier_str_mv | 10.1186/s13287-020-01977-0 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/26114467 |
| publishDate | 2020 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | NKX6.1 transcription factor: a crucial regulator of pancreatic β cell development, identity, and proliferationIdil I. Aigha (14913774)Essam M. Abdelalim (5768072)Biomedical and clinical sciencesClinical sciencesMedical biotechnologyDiabetesTranscription factorβ cell massPluripotent stem cellsPancreatic progenitorsCell therapy<p dir="ltr">Understanding the biology underlying the mechanisms and pathways regulating pancreatic β cell development is necessary to understand the pathology of diabetes mellitus (DM), which is characterized by the progressive reduction in insulin-producing β cell mass. Pluripotent stem cells (PSCs) can potentially offer an unlimited supply of functional β cells for cellular therapy and disease modeling of DM. Homeobox protein NKX6.1 is a transcription factor (TF) that plays a critical role in pancreatic β cell function and proliferation. In human pancreatic islet, NKX6.1 expression is exclusive to β cells and is undetectable in other islet cells. Several reports showed that activation of NKX6.1 in PSC-derived pancreatic progenitors (MPCs), expressing PDX1 (PDX1+/NKX6.1+), warrants their future commitment to monohormonal β cells. However, further differentiation of MPCs lacking NKX6.1 expression (PDX1+/NKX6.1−) results in an undesirable generation of non-functional polyhormonal β cells. The importance of NKX6.1 as a crucial regulator in MPC specification into functional β cells directs attentions to further investigating its mechanism and enhancing NKX6.1 expression as a means to increase β cell function and mass. Here, we shed light on the role of NKX6.1 during pancreatic β cell development and in directing the MPCs to functional monohormonal lineage. Furthermore, we address the transcriptional mechanisms and targets of NKX6.1 as well as its association with diabetes.</p><h2>Other Information</h2><p dir="ltr">Published in: Stem Cell Research & Therapy<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1186/s13287-020-01977-0" target="_blank">https://dx.doi.org/10.1186/s13287-020-01977-0</a></p>2020-10-01T00:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1186/s13287-020-01977-0https://figshare.com/articles/journal_contribution/NKX6_1_transcription_factor_a_crucial_regulator_of_pancreatic_cell_development_identity_and_proliferation/26114467CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/261144672020-10-01T00:00:00Z |
| spellingShingle | NKX6.1 transcription factor: a crucial regulator of pancreatic β cell development, identity, and proliferation Idil I. Aigha (14913774) Biomedical and clinical sciences Clinical sciences Medical biotechnology Diabetes Transcription factor β cell mass Pluripotent stem cells Pancreatic progenitors Cell therapy |
| status_str | publishedVersion |
| title | NKX6.1 transcription factor: a crucial regulator of pancreatic β cell development, identity, and proliferation |
| title_full | NKX6.1 transcription factor: a crucial regulator of pancreatic β cell development, identity, and proliferation |
| title_fullStr | NKX6.1 transcription factor: a crucial regulator of pancreatic β cell development, identity, and proliferation |
| title_full_unstemmed | NKX6.1 transcription factor: a crucial regulator of pancreatic β cell development, identity, and proliferation |
| title_short | NKX6.1 transcription factor: a crucial regulator of pancreatic β cell development, identity, and proliferation |
| title_sort | NKX6.1 transcription factor: a crucial regulator of pancreatic β cell development, identity, and proliferation |
| topic | Biomedical and clinical sciences Clinical sciences Medical biotechnology Diabetes Transcription factor β cell mass Pluripotent stem cells Pancreatic progenitors Cell therapy |