Fractionation and phytochemical composition of an ethanolic extract of Ziziphus nummularia leaves: antioxidant and anticancerous properties in human triple negative breast cancer cells
<p dir="ltr">Natural products have long been utilized in traditional medicine as remedies to improve health and treat illnesses, and have had a key role in modern drug discovery. Recently, there has been a revived interest in the search for bioactives from natural sources as alternat...
محفوظ في:
| المؤلف الرئيسي: | |
|---|---|
| مؤلفون آخرون: | , , , , , , , , |
| منشور في: |
2024
|
| الموضوعات: | |
| الوسوم: |
إضافة وسم
لا توجد وسوم, كن أول من يضع وسما على هذه التسجيلة!
|
| _version_ | 1864513534215323648 |
|---|---|
| author | Rola Abdallah (22484130) |
| author2 | Abdullah Shaito (20545181) Adnan Badran (9200140) Serine Baydoun (295978) Mansour Sobeh (5337413) Wafae Ouchari (16719270) Nihad Sahri (22484133) Ali H. Eid (5461829) Joelle Edward Mesmar (22484135) Elias Baydoun (9200155) |
| author2_role | author author author author author author author author author |
| author_facet | Rola Abdallah (22484130) Abdullah Shaito (20545181) Adnan Badran (9200140) Serine Baydoun (295978) Mansour Sobeh (5337413) Wafae Ouchari (16719270) Nihad Sahri (22484133) Ali H. Eid (5461829) Joelle Edward Mesmar (22484135) Elias Baydoun (9200155) |
| author_role | author |
| dc.creator.none.fl_str_mv | Rola Abdallah (22484130) Abdullah Shaito (20545181) Adnan Badran (9200140) Serine Baydoun (295978) Mansour Sobeh (5337413) Wafae Ouchari (16719270) Nihad Sahri (22484133) Ali H. Eid (5461829) Joelle Edward Mesmar (22484135) Elias Baydoun (9200155) |
| dc.date.none.fl_str_mv | 2024-02-09T06:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3389/fphar.2024.1331843 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Fractionation_and_phytochemical_composition_of_an_ethanolic_extract_of_Ziziphus_nummularia_leaves_antioxidant_and_anticancerous_properties_in_human_triple_negative_breast_cancer_cells/30415159 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Agricultural, veterinary and food sciences Crop and pasture production Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Oncology and carcinogenesis Pharmacology and pharmaceutical sciences Health sciences Traditional, complementary and integrative medicine Ziziphus nummularia oxidative stress ROS breast cancer herbal medicine apoptosis autophagy HPLC-PDA-MS/MS |
| dc.title.none.fl_str_mv | Fractionation and phytochemical composition of an ethanolic extract of Ziziphus nummularia leaves: antioxidant and anticancerous properties in human triple negative breast cancer cells |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Natural products have long been utilized in traditional medicine as remedies to improve health and treat illnesses, and have had a key role in modern drug discovery. Recently, there has been a revived interest in the search for bioactives from natural sources as alternative or complementary modalities to synthetic medicines; especially for cancer treatment, which incidence and mortality rates are on the rise worldwide.<i>Ziziphus nummularia</i>has been widely used in traditional medicine for the treatment of various diseases. Its traditional uses and numerous ethnopharmacological properties may be attributed to its richness in bioactive metabolites. However, its phytochemical composition or chemopreventive effects against the aggressive triple-negative breast cancer (TNBC) are still poorly explored. Here, phytochemical composition of an ethanolic extract of<i>Z. nummularia</i>leaves (ZNE) and its chromatographically isolated fractions was identified both qualitatively by spectrophotometric assays and analytically by HPLC-PDA-MS/MS. The anti-proliferative effects of ZNE were tested in several cancer cell lines, but we focused on its anti-TNBC effects since they were not explored yet. The anti-cancerous potential of ZNE and its fractions was tested<i>in vitro</i>in MDA-MB-231, a TNBC cell line. Results showed that ZNE and its Fraction 6 (F6) reduced the viability of MDA-MB-231 cells. F6 decreased MDA-MB-231 viability more than crude ZNE or its other fractions. ZNE and F6 are rich in phytochemicals and HPLC-PDA-MS/MS analysis identified several metabolites that were previously reported to have anti-cancerous effects. Both ZNE and F6 showed potent antioxidant capacity in the DPPH assay, but promoted reactive oxygen species (ROS) production in MDA-MB-231 cells; an effect which was blunted by the antioxidant N-acetyl cysteine (NAC). NAC also blunted ZNE- and F6-induced reduction in TNBC cell viability. We also demonstrated that ZNE and F6 induced an arrest of the cell cycle, and triggered apoptosis- and autophagy-mediated cell death. ZNE and F6 inhibited metastasis-related cellular processes by modifying cell migration, invasion, and adhesion. Taken together, our findings reveal that<i>Z</i>.<i>nummularia</i>is rich in phytochemicals that can attenuate the malignant phenotype of TNBC and may offer innovative avenues for the discovery of new drug leads for treatment of TNBC and other cancers.</p><h2>Other Information</h2><p dir="ltr">Published in: Frontiers in Pharmacology<br>License: <a href="https://creativecommons.org/licenses/by/4.0/deed.en" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://doi.org/10.3389/fphar.2024.1331843" target="_blank">https://doi.org/10.3389/fphar.2024.1331843</a></p><p dir="ltr"><br></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_95676b402415a9e0bd2bd8d49e510ae8 |
| identifier_str_mv | 10.3389/fphar.2024.1331843 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/30415159 |
| publishDate | 2024 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Fractionation and phytochemical composition of an ethanolic extract of Ziziphus nummularia leaves: antioxidant and anticancerous properties in human triple negative breast cancer cellsRola Abdallah (22484130)Abdullah Shaito (20545181)Adnan Badran (9200140)Serine Baydoun (295978)Mansour Sobeh (5337413)Wafae Ouchari (16719270)Nihad Sahri (22484133)Ali H. Eid (5461829)Joelle Edward Mesmar (22484135)Elias Baydoun (9200155)Agricultural, veterinary and food sciencesCrop and pasture productionBiological sciencesBiochemistry and cell biologyBiomedical and clinical sciencesOncology and carcinogenesisPharmacology and pharmaceutical sciencesHealth sciencesTraditional, complementary and integrative medicineZiziphus nummulariaoxidative stressROSbreast cancerherbal medicineapoptosisautophagyHPLC-PDA-MS/MS<p dir="ltr">Natural products have long been utilized in traditional medicine as remedies to improve health and treat illnesses, and have had a key role in modern drug discovery. Recently, there has been a revived interest in the search for bioactives from natural sources as alternative or complementary modalities to synthetic medicines; especially for cancer treatment, which incidence and mortality rates are on the rise worldwide.<i>Ziziphus nummularia</i>has been widely used in traditional medicine for the treatment of various diseases. Its traditional uses and numerous ethnopharmacological properties may be attributed to its richness in bioactive metabolites. However, its phytochemical composition or chemopreventive effects against the aggressive triple-negative breast cancer (TNBC) are still poorly explored. Here, phytochemical composition of an ethanolic extract of<i>Z. nummularia</i>leaves (ZNE) and its chromatographically isolated fractions was identified both qualitatively by spectrophotometric assays and analytically by HPLC-PDA-MS/MS. The anti-proliferative effects of ZNE were tested in several cancer cell lines, but we focused on its anti-TNBC effects since they were not explored yet. The anti-cancerous potential of ZNE and its fractions was tested<i>in vitro</i>in MDA-MB-231, a TNBC cell line. Results showed that ZNE and its Fraction 6 (F6) reduced the viability of MDA-MB-231 cells. F6 decreased MDA-MB-231 viability more than crude ZNE or its other fractions. ZNE and F6 are rich in phytochemicals and HPLC-PDA-MS/MS analysis identified several metabolites that were previously reported to have anti-cancerous effects. Both ZNE and F6 showed potent antioxidant capacity in the DPPH assay, but promoted reactive oxygen species (ROS) production in MDA-MB-231 cells; an effect which was blunted by the antioxidant N-acetyl cysteine (NAC). NAC also blunted ZNE- and F6-induced reduction in TNBC cell viability. We also demonstrated that ZNE and F6 induced an arrest of the cell cycle, and triggered apoptosis- and autophagy-mediated cell death. ZNE and F6 inhibited metastasis-related cellular processes by modifying cell migration, invasion, and adhesion. Taken together, our findings reveal that<i>Z</i>.<i>nummularia</i>is rich in phytochemicals that can attenuate the malignant phenotype of TNBC and may offer innovative avenues for the discovery of new drug leads for treatment of TNBC and other cancers.</p><h2>Other Information</h2><p dir="ltr">Published in: Frontiers in Pharmacology<br>License: <a href="https://creativecommons.org/licenses/by/4.0/deed.en" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://doi.org/10.3389/fphar.2024.1331843" target="_blank">https://doi.org/10.3389/fphar.2024.1331843</a></p><p dir="ltr"><br></p>2024-02-09T06:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3389/fphar.2024.1331843https://figshare.com/articles/journal_contribution/Fractionation_and_phytochemical_composition_of_an_ethanolic_extract_of_Ziziphus_nummularia_leaves_antioxidant_and_anticancerous_properties_in_human_triple_negative_breast_cancer_cells/30415159CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/304151592024-02-09T06:00:00Z |
| spellingShingle | Fractionation and phytochemical composition of an ethanolic extract of Ziziphus nummularia leaves: antioxidant and anticancerous properties in human triple negative breast cancer cells Rola Abdallah (22484130) Agricultural, veterinary and food sciences Crop and pasture production Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Oncology and carcinogenesis Pharmacology and pharmaceutical sciences Health sciences Traditional, complementary and integrative medicine Ziziphus nummularia oxidative stress ROS breast cancer herbal medicine apoptosis autophagy HPLC-PDA-MS/MS |
| status_str | publishedVersion |
| title | Fractionation and phytochemical composition of an ethanolic extract of Ziziphus nummularia leaves: antioxidant and anticancerous properties in human triple negative breast cancer cells |
| title_full | Fractionation and phytochemical composition of an ethanolic extract of Ziziphus nummularia leaves: antioxidant and anticancerous properties in human triple negative breast cancer cells |
| title_fullStr | Fractionation and phytochemical composition of an ethanolic extract of Ziziphus nummularia leaves: antioxidant and anticancerous properties in human triple negative breast cancer cells |
| title_full_unstemmed | Fractionation and phytochemical composition of an ethanolic extract of Ziziphus nummularia leaves: antioxidant and anticancerous properties in human triple negative breast cancer cells |
| title_short | Fractionation and phytochemical composition of an ethanolic extract of Ziziphus nummularia leaves: antioxidant and anticancerous properties in human triple negative breast cancer cells |
| title_sort | Fractionation and phytochemical composition of an ethanolic extract of Ziziphus nummularia leaves: antioxidant and anticancerous properties in human triple negative breast cancer cells |
| topic | Agricultural, veterinary and food sciences Crop and pasture production Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Oncology and carcinogenesis Pharmacology and pharmaceutical sciences Health sciences Traditional, complementary and integrative medicine Ziziphus nummularia oxidative stress ROS breast cancer herbal medicine apoptosis autophagy HPLC-PDA-MS/MS |