Simultaneous Multi-Adulterant Detection in Milk Using an In-house Developed Multiplexed Microfluidic Paper-Based Analytical Device (M-µPAD)

<p dir="ltr">Milk adulteration poses a significant challenge in developing countries, impacting food safety and public health. This study introduces a multiplexed microfluidic paper-based analytical device (M-µPAD) for rapid and simultaneous detection of multiple milk adulterants. Th...

وصف كامل

محفوظ في:
التفاصيل البيبلوغرافية
المؤلف الرئيسي: Jannat Ahsan (22466530) (author)
مؤلفون آخرون: Kashif Riaz (20944418) (author), Hudair Samad (22466533) (author), Muhammad Qasim Mehmood (9514383) (author), Abdelkrim Khelif (4422280) (author)
منشور في: 2025
الموضوعات:
الوسوم: إضافة وسم
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author Jannat Ahsan (22466530)
author2 Kashif Riaz (20944418)
Hudair Samad (22466533)
Muhammad Qasim Mehmood (9514383)
Abdelkrim Khelif (4422280)
author2_role author
author
author
author
author_facet Jannat Ahsan (22466530)
Kashif Riaz (20944418)
Hudair Samad (22466533)
Muhammad Qasim Mehmood (9514383)
Abdelkrim Khelif (4422280)
author_role author
dc.creator.none.fl_str_mv Jannat Ahsan (22466530)
Kashif Riaz (20944418)
Hudair Samad (22466533)
Muhammad Qasim Mehmood (9514383)
Abdelkrim Khelif (4422280)
dc.date.none.fl_str_mv 2025-05-15T09:00:00Z
dc.identifier.none.fl_str_mv 10.1007/s11220-025-00580-y
dc.relation.none.fl_str_mv https://figshare.com/articles/journal_contribution/Simultaneous_Multi-Adulterant_Detection_in_Milk_Using_an_In-house_Developed_Multiplexed_Microfluidic_Paper-Based_Analytical_Device_M-_PAD_/30405481
dc.rights.none.fl_str_mv CC BY 4.0
info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Agricultural, veterinary and food sciences
Food sciences
Health sciences
Public health
Multiplexed µPADs
Colorimetric
Analysis Milk adulteration detection
Resource-limited diagnostics
Low-cost fabrication
dc.title.none.fl_str_mv Simultaneous Multi-Adulterant Detection in Milk Using an In-house Developed Multiplexed Microfluidic Paper-Based Analytical Device (M-µPAD)
dc.type.none.fl_str_mv Text
Journal contribution
info:eu-repo/semantics/publishedVersion
text
contribution to journal
description <p dir="ltr">Milk adulteration poses a significant challenge in developing countries, impacting food safety and public health. This study introduces a multiplexed microfluidic paper-based analytical device (M-µPAD) for rapid and simultaneous detection of multiple milk adulterants. The M-µPAD, fabricated using readily available materials like filter paper and wax crayons, eliminates the need for specialized equipment. The device demonstrates linear relationships between colorimetric response and adulterant concentration for urea (10–200 mg/dL), starch (20-1000 mg/dL), and detergent (100–3000 mg/dL), with detection limits of 11.1, 22.4, and 120.8 mg/dL, respectively. Requiring only 40 µL of reagents and 80 µL of milk sample, the M-µPAD achieves over 95% accuracy and reproducibility with RSD values below 4%. This affordable, in-house fabricated platform offers a promising solution for milk quality assessment in resource-limited settings, addressing the need for accessible and efficient adulterant detection methods.</p><h2>Other Information</h2><p dir="ltr">Published in: Sensing and Imaging<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1007/s11220-025-00580-y" target="_blank">https://dx.doi.org/10.1007/s11220-025-00580-y</a></p>
eu_rights_str_mv openAccess
id Manara2_9a224b7d88b7ad997621795597bfb39d
identifier_str_mv 10.1007/s11220-025-00580-y
network_acronym_str Manara2
network_name_str Manara2
oai_identifier_str oai:figshare.com:article/30405481
publishDate 2025
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rights_invalid_str_mv CC BY 4.0
spelling Simultaneous Multi-Adulterant Detection in Milk Using an In-house Developed Multiplexed Microfluidic Paper-Based Analytical Device (M-µPAD)Jannat Ahsan (22466530)Kashif Riaz (20944418)Hudair Samad (22466533)Muhammad Qasim Mehmood (9514383)Abdelkrim Khelif (4422280)Agricultural, veterinary and food sciencesFood sciencesHealth sciencesPublic healthMultiplexed µPADsColorimetricAnalysis Milk adulteration detectionResource-limited diagnosticsLow-cost fabrication<p dir="ltr">Milk adulteration poses a significant challenge in developing countries, impacting food safety and public health. This study introduces a multiplexed microfluidic paper-based analytical device (M-µPAD) for rapid and simultaneous detection of multiple milk adulterants. The M-µPAD, fabricated using readily available materials like filter paper and wax crayons, eliminates the need for specialized equipment. The device demonstrates linear relationships between colorimetric response and adulterant concentration for urea (10–200 mg/dL), starch (20-1000 mg/dL), and detergent (100–3000 mg/dL), with detection limits of 11.1, 22.4, and 120.8 mg/dL, respectively. Requiring only 40 µL of reagents and 80 µL of milk sample, the M-µPAD achieves over 95% accuracy and reproducibility with RSD values below 4%. This affordable, in-house fabricated platform offers a promising solution for milk quality assessment in resource-limited settings, addressing the need for accessible and efficient adulterant detection methods.</p><h2>Other Information</h2><p dir="ltr">Published in: Sensing and Imaging<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1007/s11220-025-00580-y" target="_blank">https://dx.doi.org/10.1007/s11220-025-00580-y</a></p>2025-05-15T09:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1007/s11220-025-00580-yhttps://figshare.com/articles/journal_contribution/Simultaneous_Multi-Adulterant_Detection_in_Milk_Using_an_In-house_Developed_Multiplexed_Microfluidic_Paper-Based_Analytical_Device_M-_PAD_/30405481CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/304054812025-05-15T09:00:00Z
spellingShingle Simultaneous Multi-Adulterant Detection in Milk Using an In-house Developed Multiplexed Microfluidic Paper-Based Analytical Device (M-µPAD)
Jannat Ahsan (22466530)
Agricultural, veterinary and food sciences
Food sciences
Health sciences
Public health
Multiplexed µPADs
Colorimetric
Analysis Milk adulteration detection
Resource-limited diagnostics
Low-cost fabrication
status_str publishedVersion
title Simultaneous Multi-Adulterant Detection in Milk Using an In-house Developed Multiplexed Microfluidic Paper-Based Analytical Device (M-µPAD)
title_full Simultaneous Multi-Adulterant Detection in Milk Using an In-house Developed Multiplexed Microfluidic Paper-Based Analytical Device (M-µPAD)
title_fullStr Simultaneous Multi-Adulterant Detection in Milk Using an In-house Developed Multiplexed Microfluidic Paper-Based Analytical Device (M-µPAD)
title_full_unstemmed Simultaneous Multi-Adulterant Detection in Milk Using an In-house Developed Multiplexed Microfluidic Paper-Based Analytical Device (M-µPAD)
title_short Simultaneous Multi-Adulterant Detection in Milk Using an In-house Developed Multiplexed Microfluidic Paper-Based Analytical Device (M-µPAD)
title_sort Simultaneous Multi-Adulterant Detection in Milk Using an In-house Developed Multiplexed Microfluidic Paper-Based Analytical Device (M-µPAD)
topic Agricultural, veterinary and food sciences
Food sciences
Health sciences
Public health
Multiplexed µPADs
Colorimetric
Analysis Milk adulteration detection
Resource-limited diagnostics
Low-cost fabrication