Yeast Lacking the PP2A Phosphatase Regulatory Subunit Rts1 Sensitizes rad51 Mutants to Specific DNA Damaging Agents
<p dir="ltr">Rts1 is a regulatory subunit of the trimeric protein phosphatase 2A phosphatase and it participates in many biological processes by modulating the phosphorylation status of proteins. Consistent with its role, mutants lacking Rts1 display multiple phenotypes. We have prev...
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2019
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| _version_ | 1864513515131240448 |
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| author | Mustapha Aouida (417652) |
| author2 | Abdelmoez Eshrif (7839065) Dindial Ramotar (208416) |
| author2_role | author author |
| author_facet | Mustapha Aouida (417652) Abdelmoez Eshrif (7839065) Dindial Ramotar (208416) |
| author_role | author |
| dc.creator.none.fl_str_mv | Mustapha Aouida (417652) Abdelmoez Eshrif (7839065) Dindial Ramotar (208416) |
| dc.date.none.fl_str_mv | 2019-11-08T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3389/fgene.2019.01117 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Yeast_Lacking_the_PP2A_Phosphatase_Regulatory_Subunit_Rts1_Sensitizes_rad51_Mutants_to_Specific_DNA_Damaging_Agents/25886923 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Genetics anticancer drug bleomycin mutagenesis phosphatase subunit DNA damage yeast |
| dc.title.none.fl_str_mv | Yeast Lacking the PP2A Phosphatase Regulatory Subunit Rts1 Sensitizes rad51 Mutants to Specific DNA Damaging Agents |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Rts1 is a regulatory subunit of the trimeric protein phosphatase 2A phosphatase and it participates in many biological processes by modulating the phosphorylation status of proteins. Consistent with its role, mutants lacking Rts1 display multiple phenotypes. We have previously performed a high throughput screen to search for yeast haploid mutants with altered sensitivity to the anticancer drug bleomycin, which acts by damaging the DNA to produce single and double strand breaks. <i>RTS1</i> was among the genes that when singly deleted cause sensitivity to bleomycin. We investigate whether Rts1 plays a role in the repair of bleomycin-induced DNA lesions. We show that deletion of the <i>RTS1</i> gene in the <i>rad51</i> null background, lacking Rad51 known to be involved in the repair of bleomycin-induced DNA lesions, resulted in double mutants that were sensitized to bleomycin and not to other DNA damaging agents that creates DNA adducts. We further show that Rts1 has the ability to bind to DNA and in its absence cells displayed an increase in the frequency of both spontaneous and bleomycin-induced mutations compared to the parent. This is the first report implicating Rts1 with a role in DNA damage and repair, perhaps regulating the phosphorylation status of one or more proteins involved in the repair of DNA strand breaks.</p><h2>Other Information</h2><p dir="ltr">Published in: Frontiers in Genetics<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3389/fgene.2019.01117" target="_blank">https://dx.doi.org/10.3389/fgene.2019.01117</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_9b0e4d869b2663231073ba8490ebe984 |
| identifier_str_mv | 10.3389/fgene.2019.01117 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25886923 |
| publishDate | 2019 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Yeast Lacking the PP2A Phosphatase Regulatory Subunit Rts1 Sensitizes rad51 Mutants to Specific DNA Damaging AgentsMustapha Aouida (417652)Abdelmoez Eshrif (7839065)Dindial Ramotar (208416)Biological sciencesGeneticsanticancer drug bleomycinmutagenesisphosphatase subunitDNA damageyeast<p dir="ltr">Rts1 is a regulatory subunit of the trimeric protein phosphatase 2A phosphatase and it participates in many biological processes by modulating the phosphorylation status of proteins. Consistent with its role, mutants lacking Rts1 display multiple phenotypes. We have previously performed a high throughput screen to search for yeast haploid mutants with altered sensitivity to the anticancer drug bleomycin, which acts by damaging the DNA to produce single and double strand breaks. <i>RTS1</i> was among the genes that when singly deleted cause sensitivity to bleomycin. We investigate whether Rts1 plays a role in the repair of bleomycin-induced DNA lesions. We show that deletion of the <i>RTS1</i> gene in the <i>rad51</i> null background, lacking Rad51 known to be involved in the repair of bleomycin-induced DNA lesions, resulted in double mutants that were sensitized to bleomycin and not to other DNA damaging agents that creates DNA adducts. We further show that Rts1 has the ability to bind to DNA and in its absence cells displayed an increase in the frequency of both spontaneous and bleomycin-induced mutations compared to the parent. This is the first report implicating Rts1 with a role in DNA damage and repair, perhaps regulating the phosphorylation status of one or more proteins involved in the repair of DNA strand breaks.</p><h2>Other Information</h2><p dir="ltr">Published in: Frontiers in Genetics<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3389/fgene.2019.01117" target="_blank">https://dx.doi.org/10.3389/fgene.2019.01117</a></p>2019-11-08T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3389/fgene.2019.01117https://figshare.com/articles/journal_contribution/Yeast_Lacking_the_PP2A_Phosphatase_Regulatory_Subunit_Rts1_Sensitizes_rad51_Mutants_to_Specific_DNA_Damaging_Agents/25886923CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/258869232019-11-08T03:00:00Z |
| spellingShingle | Yeast Lacking the PP2A Phosphatase Regulatory Subunit Rts1 Sensitizes rad51 Mutants to Specific DNA Damaging Agents Mustapha Aouida (417652) Biological sciences Genetics anticancer drug bleomycin mutagenesis phosphatase subunit DNA damage yeast |
| status_str | publishedVersion |
| title | Yeast Lacking the PP2A Phosphatase Regulatory Subunit Rts1 Sensitizes rad51 Mutants to Specific DNA Damaging Agents |
| title_full | Yeast Lacking the PP2A Phosphatase Regulatory Subunit Rts1 Sensitizes rad51 Mutants to Specific DNA Damaging Agents |
| title_fullStr | Yeast Lacking the PP2A Phosphatase Regulatory Subunit Rts1 Sensitizes rad51 Mutants to Specific DNA Damaging Agents |
| title_full_unstemmed | Yeast Lacking the PP2A Phosphatase Regulatory Subunit Rts1 Sensitizes rad51 Mutants to Specific DNA Damaging Agents |
| title_short | Yeast Lacking the PP2A Phosphatase Regulatory Subunit Rts1 Sensitizes rad51 Mutants to Specific DNA Damaging Agents |
| title_sort | Yeast Lacking the PP2A Phosphatase Regulatory Subunit Rts1 Sensitizes rad51 Mutants to Specific DNA Damaging Agents |
| topic | Biological sciences Genetics anticancer drug bleomycin mutagenesis phosphatase subunit DNA damage yeast |