Preparation, characterization, in vitro drug release and anti-inflammatory of thymoquinone-loaded chitosan nanocomposite

<p dir="ltr">In this study, we formulated Thymoquinone-loaded nanocomposites (TQ-NCs) using high-pressure homogenizer without <u>sodium tripolyphosphate</u>. The TQ-NCs were characterized and their anti-inflammatory determined by the response of the LPS-stimulated macroph...

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محفوظ في:
التفاصيل البيبلوغرافية
المؤلف الرئيسي: Mothanna Sadiq Al-Qubaisi (17844218) (author)
مؤلفون آخرون: Ashwaq Shakir Al-Abboodi (21323177) (author), Fatah H. Alhassan (17844212) (author), Samer Hussein-Al-Ali (21323180) (author), Moayad Husein Flaifel (21323183) (author), Eltayeb E.M. Eid (20402225) (author), Hussah Abdullah Alshwyeh (16621140) (author), Mohd Zobir Hussein (5558990) (author), Sulaiman Mohammed Alnasser (16713520) (author), Mohammed Ibrahim Saeed (21323186) (author), Abdullah Rasedee (6940799) (author), Wisam Nabeel Ibrahim (13899337) (author)
منشور في: 2022
الموضوعات:
الوسوم: إضافة وسم
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author Mothanna Sadiq Al-Qubaisi (17844218)
author2 Ashwaq Shakir Al-Abboodi (21323177)
Fatah H. Alhassan (17844212)
Samer Hussein-Al-Ali (21323180)
Moayad Husein Flaifel (21323183)
Eltayeb E.M. Eid (20402225)
Hussah Abdullah Alshwyeh (16621140)
Mohd Zobir Hussein (5558990)
Sulaiman Mohammed Alnasser (16713520)
Mohammed Ibrahim Saeed (21323186)
Abdullah Rasedee (6940799)
Wisam Nabeel Ibrahim (13899337)
author2_role author
author
author
author
author
author
author
author
author
author
author
author_facet Mothanna Sadiq Al-Qubaisi (17844218)
Ashwaq Shakir Al-Abboodi (21323177)
Fatah H. Alhassan (17844212)
Samer Hussein-Al-Ali (21323180)
Moayad Husein Flaifel (21323183)
Eltayeb E.M. Eid (20402225)
Hussah Abdullah Alshwyeh (16621140)
Mohd Zobir Hussein (5558990)
Sulaiman Mohammed Alnasser (16713520)
Mohammed Ibrahim Saeed (21323186)
Abdullah Rasedee (6940799)
Wisam Nabeel Ibrahim (13899337)
author_role author
dc.creator.none.fl_str_mv Mothanna Sadiq Al-Qubaisi (17844218)
Ashwaq Shakir Al-Abboodi (21323177)
Fatah H. Alhassan (17844212)
Samer Hussein-Al-Ali (21323180)
Moayad Husein Flaifel (21323183)
Eltayeb E.M. Eid (20402225)
Hussah Abdullah Alshwyeh (16621140)
Mohd Zobir Hussein (5558990)
Sulaiman Mohammed Alnasser (16713520)
Mohammed Ibrahim Saeed (21323186)
Abdullah Rasedee (6940799)
Wisam Nabeel Ibrahim (13899337)
dc.date.none.fl_str_mv 2022-04-27T09:00:00Z
dc.identifier.none.fl_str_mv 10.1016/j.jsps.2022.02.002
dc.relation.none.fl_str_mv https://figshare.com/articles/journal_contribution/Preparation_characterization_in_vitro_drug_release_and_anti-inflammatory_of_thymoquinone-loaded_chitosan_nanocomposite/29021459
dc.rights.none.fl_str_mv CC BY 4.0
info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Biomedical and clinical sciences
Pharmacology and pharmaceutical sciences
Thymoquinone
Nanocomposite
High-pressure homogenizer
Drug release
RAW 264.7 cells
Inflammatory mediators
dc.title.none.fl_str_mv Preparation, characterization, in vitro drug release and anti-inflammatory of thymoquinone-loaded chitosan nanocomposite
dc.type.none.fl_str_mv Text
Journal contribution
info:eu-repo/semantics/publishedVersion
text
contribution to journal
description <p dir="ltr">In this study, we formulated Thymoquinone-loaded nanocomposites (TQ-NCs) using high-pressure homogenizer without <u>sodium tripolyphosphate</u>. The TQ-NCs were characterized and their anti-inflammatory determined by the response of the LPS-stimulated macrophage RAW 264.7 cells in the production of <u>nitric oxide,</u> <u>prostaglandin E2</u>, tumor necrosis factor-α, interleukin-6, and interleukin-1β. The physicochemical properties of TQ-NC were determined using different machines. TQ was fully incorporated in the highly thermal stable<u> nanoparticles</u>. The nanoparticles showed rapid release of TQ in the acidic medium of the gastric juice. In medium of pH 6.8, TQ-NC exhibited sustained release of TQ over a period of 100 h. The results suggest that TQ-NC nanoparticles have potential application as parenterally administered therapeutic compound. TQ-NC effectively reduce production of inflammatory cytokines by the LPS-stimulated RAW 264.7 cells, indicating that they have anti-inflammatory properties. In conclusion, TQ-NC nanoparticles have the characteristics of efficient carrier for TQ and an effective anti-inflammatory therapeutic compound.</p><h2>Other Information</h2><p dir="ltr">Published in: Saudi Pharmaceutical Journal<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.jsps.2022.02.002" target="_blank">https://dx.doi.org/10.1016/j.jsps.2022.02.002</a></p>
eu_rights_str_mv openAccess
id Manara2_9e2c78658e73736db52045705aa899d6
identifier_str_mv 10.1016/j.jsps.2022.02.002
network_acronym_str Manara2
network_name_str Manara2
oai_identifier_str oai:figshare.com:article/29021459
publishDate 2022
repository.mail.fl_str_mv
repository.name.fl_str_mv
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rights_invalid_str_mv CC BY 4.0
spelling Preparation, characterization, in vitro drug release and anti-inflammatory of thymoquinone-loaded chitosan nanocompositeMothanna Sadiq Al-Qubaisi (17844218)Ashwaq Shakir Al-Abboodi (21323177)Fatah H. Alhassan (17844212)Samer Hussein-Al-Ali (21323180)Moayad Husein Flaifel (21323183)Eltayeb E.M. Eid (20402225)Hussah Abdullah Alshwyeh (16621140)Mohd Zobir Hussein (5558990)Sulaiman Mohammed Alnasser (16713520)Mohammed Ibrahim Saeed (21323186)Abdullah Rasedee (6940799)Wisam Nabeel Ibrahim (13899337)Biomedical and clinical sciencesPharmacology and pharmaceutical sciencesThymoquinoneNanocompositeHigh-pressure homogenizerDrug releaseRAW 264.7 cellsInflammatory mediators<p dir="ltr">In this study, we formulated Thymoquinone-loaded nanocomposites (TQ-NCs) using high-pressure homogenizer without <u>sodium tripolyphosphate</u>. The TQ-NCs were characterized and their anti-inflammatory determined by the response of the LPS-stimulated macrophage RAW 264.7 cells in the production of <u>nitric oxide,</u> <u>prostaglandin E2</u>, tumor necrosis factor-α, interleukin-6, and interleukin-1β. The physicochemical properties of TQ-NC were determined using different machines. TQ was fully incorporated in the highly thermal stable<u> nanoparticles</u>. The nanoparticles showed rapid release of TQ in the acidic medium of the gastric juice. In medium of pH 6.8, TQ-NC exhibited sustained release of TQ over a period of 100 h. The results suggest that TQ-NC nanoparticles have potential application as parenterally administered therapeutic compound. TQ-NC effectively reduce production of inflammatory cytokines by the LPS-stimulated RAW 264.7 cells, indicating that they have anti-inflammatory properties. In conclusion, TQ-NC nanoparticles have the characteristics of efficient carrier for TQ and an effective anti-inflammatory therapeutic compound.</p><h2>Other Information</h2><p dir="ltr">Published in: Saudi Pharmaceutical Journal<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.jsps.2022.02.002" target="_blank">https://dx.doi.org/10.1016/j.jsps.2022.02.002</a></p>2022-04-27T09:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1016/j.jsps.2022.02.002https://figshare.com/articles/journal_contribution/Preparation_characterization_in_vitro_drug_release_and_anti-inflammatory_of_thymoquinone-loaded_chitosan_nanocomposite/29021459CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/290214592022-04-27T09:00:00Z
spellingShingle Preparation, characterization, in vitro drug release and anti-inflammatory of thymoquinone-loaded chitosan nanocomposite
Mothanna Sadiq Al-Qubaisi (17844218)
Biomedical and clinical sciences
Pharmacology and pharmaceutical sciences
Thymoquinone
Nanocomposite
High-pressure homogenizer
Drug release
RAW 264.7 cells
Inflammatory mediators
status_str publishedVersion
title Preparation, characterization, in vitro drug release and anti-inflammatory of thymoquinone-loaded chitosan nanocomposite
title_full Preparation, characterization, in vitro drug release and anti-inflammatory of thymoquinone-loaded chitosan nanocomposite
title_fullStr Preparation, characterization, in vitro drug release and anti-inflammatory of thymoquinone-loaded chitosan nanocomposite
title_full_unstemmed Preparation, characterization, in vitro drug release and anti-inflammatory of thymoquinone-loaded chitosan nanocomposite
title_short Preparation, characterization, in vitro drug release and anti-inflammatory of thymoquinone-loaded chitosan nanocomposite
title_sort Preparation, characterization, in vitro drug release and anti-inflammatory of thymoquinone-loaded chitosan nanocomposite
topic Biomedical and clinical sciences
Pharmacology and pharmaceutical sciences
Thymoquinone
Nanocomposite
High-pressure homogenizer
Drug release
RAW 264.7 cells
Inflammatory mediators