Downregulation of <i>CYP17A1</i> by 20-hydroxyecdysone: plasma progesterone and its vasodilatory properties
<p dir="ltr">Aim: To investigate the effect of 20-hydroxyecdysone on steroidogenic pathway genes and plasma progesterone, and its potential impact on vascular functions. Methods: Chimeric mice with humanized liver were treated with 20-hydroxyecdysone for 3 days, and hepatic steroidog...
محفوظ في:
| المؤلف الرئيسي: | |
|---|---|
| مؤلفون آخرون: | , , , , , , , , , , |
| منشور في: |
2022
|
| الموضوعات: | |
| الوسوم: |
إضافة وسم
لا توجد وسوم, كن أول من يضع وسما على هذه التسجيلة!
|
| _version_ | 1864513510789087232 |
|---|---|
| author | Maneera Y Aljaber (18278971) |
| author2 | Nelson N Orie (18278974) Asmaa Raees (18278977) Suhail Kraiem (18278980) Mashael Al-Jaber (18278983) Waseem Samsam (12204880) Mostafa M Hamza (18278986) David Abraham (36404) Norman M Kneteman (18278989) Alka Beotra (14780368) Vidya Mohamed-Ali (12204895) Mohammed Almaadheed (18278992) |
| author2_role | author author author author author author author author author author author |
| author_facet | Maneera Y Aljaber (18278971) Nelson N Orie (18278974) Asmaa Raees (18278977) Suhail Kraiem (18278980) Mashael Al-Jaber (18278983) Waseem Samsam (12204880) Mostafa M Hamza (18278986) David Abraham (36404) Norman M Kneteman (18278989) Alka Beotra (14780368) Vidya Mohamed-Ali (12204895) Mohammed Almaadheed (18278992) |
| author_role | author |
| dc.creator.none.fl_str_mv | Maneera Y Aljaber (18278971) Nelson N Orie (18278974) Asmaa Raees (18278977) Suhail Kraiem (18278980) Mashael Al-Jaber (18278983) Waseem Samsam (12204880) Mostafa M Hamza (18278986) David Abraham (36404) Norman M Kneteman (18278989) Alka Beotra (14780368) Vidya Mohamed-Ali (12204895) Mohammed Almaadheed (18278992) |
| dc.date.none.fl_str_mv | 2022-07-07T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.2144/fsoa-2022-0006 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Downregulation_of_i_CYP17A1_i_by_20-hydroxyecdysone_plasma_progesterone_and_its_vasodilatory_properties/25514029 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Clinical sciences Medical biotechnology Pharmacology and pharmaceutical sciences 20-hydroxyecdysone CYP17A1 ecdysteroid hepatic transcriptome humanized liver mesenteric arteriole muscle arteriole progesterone vasodilation voltage-dependent calcium entry |
| dc.title.none.fl_str_mv | Downregulation of <i>CYP17A1</i> by 20-hydroxyecdysone: plasma progesterone and its vasodilatory properties |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Aim: To investigate the effect of 20-hydroxyecdysone on steroidogenic pathway genes and plasma progesterone, and its potential impact on vascular functions. Methods: Chimeric mice with humanized liver were treated with 20-hydroxyecdysone for 3 days, and hepatic steroidogenic pathway genes and plasma progesterone were measured by transcriptomics and GC–MS/MS, respectively. Direct effects on muscle and mesenteric arterioles were assessed by myography. Results: CYP17A1 was downregulated in 20-hydroxyecdysone-treated mice compared with untreated group (p = 0.04), with an insignificant increase in plasma progesterone. Progesterone caused vasorelaxation which was blocked by 60 mM KCl, but unaffected by nitric oxide synthase inhibition. Conclusion: In the short term, 20-hydroxyecdysone mediates CYP17A1 downregulation without a significant increase in plasma progesterone, which has a vasodilatory effect involving inhibition of voltage-dependent calcium channels, and the potential to enhance 20-hydroxyecdysone vasorelaxation.</p><h2>Other Information</h2><p dir="ltr">Published in: Future Science OA<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.2144/fsoa-2022-0006" target="_blank">https://dx.doi.org/10.2144/fsoa-2022-0006</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_9f504ddb57487be5b606287e166058a8 |
| identifier_str_mv | 10.2144/fsoa-2022-0006 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25514029 |
| publishDate | 2022 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Downregulation of <i>CYP17A1</i> by 20-hydroxyecdysone: plasma progesterone and its vasodilatory propertiesManeera Y Aljaber (18278971)Nelson N Orie (18278974)Asmaa Raees (18278977)Suhail Kraiem (18278980)Mashael Al-Jaber (18278983)Waseem Samsam (12204880)Mostafa M Hamza (18278986)David Abraham (36404)Norman M Kneteman (18278989)Alka Beotra (14780368)Vidya Mohamed-Ali (12204895)Mohammed Almaadheed (18278992)Biomedical and clinical sciencesClinical sciencesMedical biotechnologyPharmacology and pharmaceutical sciences20-hydroxyecdysoneCYP17A1ecdysteroidhepatic transcriptomehumanized livermesenteric arteriolemuscle arterioleprogesteronevasodilationvoltage-dependent calcium entry<p dir="ltr">Aim: To investigate the effect of 20-hydroxyecdysone on steroidogenic pathway genes and plasma progesterone, and its potential impact on vascular functions. Methods: Chimeric mice with humanized liver were treated with 20-hydroxyecdysone for 3 days, and hepatic steroidogenic pathway genes and plasma progesterone were measured by transcriptomics and GC–MS/MS, respectively. Direct effects on muscle and mesenteric arterioles were assessed by myography. Results: CYP17A1 was downregulated in 20-hydroxyecdysone-treated mice compared with untreated group (p = 0.04), with an insignificant increase in plasma progesterone. Progesterone caused vasorelaxation which was blocked by 60 mM KCl, but unaffected by nitric oxide synthase inhibition. Conclusion: In the short term, 20-hydroxyecdysone mediates CYP17A1 downregulation without a significant increase in plasma progesterone, which has a vasodilatory effect involving inhibition of voltage-dependent calcium channels, and the potential to enhance 20-hydroxyecdysone vasorelaxation.</p><h2>Other Information</h2><p dir="ltr">Published in: Future Science OA<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.2144/fsoa-2022-0006" target="_blank">https://dx.doi.org/10.2144/fsoa-2022-0006</a></p>2022-07-07T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.2144/fsoa-2022-0006https://figshare.com/articles/journal_contribution/Downregulation_of_i_CYP17A1_i_by_20-hydroxyecdysone_plasma_progesterone_and_its_vasodilatory_properties/25514029CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/255140292022-07-07T03:00:00Z |
| spellingShingle | Downregulation of <i>CYP17A1</i> by 20-hydroxyecdysone: plasma progesterone and its vasodilatory properties Maneera Y Aljaber (18278971) Biomedical and clinical sciences Clinical sciences Medical biotechnology Pharmacology and pharmaceutical sciences 20-hydroxyecdysone CYP17A1 ecdysteroid hepatic transcriptome humanized liver mesenteric arteriole muscle arteriole progesterone vasodilation voltage-dependent calcium entry |
| status_str | publishedVersion |
| title | Downregulation of <i>CYP17A1</i> by 20-hydroxyecdysone: plasma progesterone and its vasodilatory properties |
| title_full | Downregulation of <i>CYP17A1</i> by 20-hydroxyecdysone: plasma progesterone and its vasodilatory properties |
| title_fullStr | Downregulation of <i>CYP17A1</i> by 20-hydroxyecdysone: plasma progesterone and its vasodilatory properties |
| title_full_unstemmed | Downregulation of <i>CYP17A1</i> by 20-hydroxyecdysone: plasma progesterone and its vasodilatory properties |
| title_short | Downregulation of <i>CYP17A1</i> by 20-hydroxyecdysone: plasma progesterone and its vasodilatory properties |
| title_sort | Downregulation of <i>CYP17A1</i> by 20-hydroxyecdysone: plasma progesterone and its vasodilatory properties |
| topic | Biomedical and clinical sciences Clinical sciences Medical biotechnology Pharmacology and pharmaceutical sciences 20-hydroxyecdysone CYP17A1 ecdysteroid hepatic transcriptome humanized liver mesenteric arteriole muscle arteriole progesterone vasodilation voltage-dependent calcium entry |