Antibacterial and Antibiofilm Activity of Mercaptophenol Functionalized-Gold Nanorods Against a Clinical Isolate of Methicillin-Resistant Staphylococcus aureus
<p>Gold nanorods (AuNRs) were synthesized by the seed-mediated wet chemical method using a binary surfactant system. AuNRs were stabilized with polyethylene glycol, then functionalized with 4-mercaptophenol (4-MPH) ligand by surface ligand exchange. The surface-functionalized AuNRs (4-MPH-AuNR...
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| مؤلفون آخرون: | , , , , |
| منشور في: |
2022
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| _version_ | 1864513567607226368 |
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| author | Nahla O. Eltai (9291053) |
| author2 | Nouf N. Mahmoud (9133074) Zain Z. Zakaria (6695093) Hana Abdelrahman (14151264) Ala-Eddin Al Moustafa (5472368) Maha Al-Asmakh (14151267) |
| author2_role | author author author author author |
| author_facet | Nahla O. Eltai (9291053) Nouf N. Mahmoud (9133074) Zain Z. Zakaria (6695093) Hana Abdelrahman (14151264) Ala-Eddin Al Moustafa (5472368) Maha Al-Asmakh (14151267) |
| author_role | author |
| dc.creator.none.fl_str_mv | Nahla O. Eltai (9291053) Nouf N. Mahmoud (9133074) Zain Z. Zakaria (6695093) Hana Abdelrahman (14151264) Ala-Eddin Al Moustafa (5472368) Maha Al-Asmakh (14151267) |
| dc.date.none.fl_str_mv | 2022-11-22T21:13:39Z |
| dc.identifier.none.fl_str_mv | 10.1007/s10904-022-02294-0 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Antibacterial_and_Antibiofilm_Activity_of_Mercaptophenol_Functionalized-Gold_Nanorods_Against_a_Clinical_Isolate_of_Methicillin-Resistant_Staphylococcus_aureus/21597351 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Macromolecular and materials chemistry Materials engineering Materials Chemistry Polymers and Plastics |
| dc.title.none.fl_str_mv | Antibacterial and Antibiofilm Activity of Mercaptophenol Functionalized-Gold Nanorods Against a Clinical Isolate of Methicillin-Resistant Staphylococcus aureus |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p>Gold nanorods (AuNRs) were synthesized by the seed-mediated wet chemical method using a binary surfactant system. AuNRs were stabilized with polyethylene glycol, then functionalized with 4-mercaptophenol (4-MPH) ligand by surface ligand exchange. The surface-functionalized AuNRs (4-MPH-AuNRs) exhibited a typical UV–vis spectrum of AuNRs with a slightly shifted longitudinal peak. Furthermore, 4-MPH-AuNRs demonstrated a similar Fourier-Transformed Infrared spectrum to 4-MPH and a fading of the thiol band, which suggests a successful functionalization through thiol-gold binding. The antibacterial and antibiofilm activities of 4-MPH-AuNRs were evaluated against a clinical isolate of Methicillin-Resistant Staphylococcus aureus (MRSA). The results indicate that 4-MPH-AuNRs exhibit a bactericidal activity with a minimum inhibitory concentration (MIC) of ~ 6.25 $$\upmu$$ μ g/mL against a planktonic suspension of MRSA. Furthermore, 4-MPH-AuNRs resulted in a 1.8–2.9 log-cycle reduction of MRSA biofilm viable count over a concentration range of 100–6.0 $$\upmu$$ μ g/mL. The bacterial uptake of the surface-modified nanorods was investigated by inductively coupled plasma-optical emission spectroscopy (ICP-OES) and scanning electron microscopy (SEM) imaging; the results reveal that the nanorods were internalized into the bacterial cells after 6 h (h) of exposure. SEM imaging revealed a significant accumulation of the nanorods at the bacterial cell wall and a possible cellular internalization. Thus, 4-MPH-AuNRs can be considered a potential antibacterial agent, particularly against MRSA strain biofilms.</p><h2>Other Information</h2> <p> Published in: Journal of Inorganic and Organometallic Polymers and Materials<br> License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="http://dx.doi.org/10.1007/s10904-022-02294-0" target="_blank">http://dx.doi.org/10.1007/s10904-022-02294-0</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_a1471f4bc3fd03241d1219e251e856f1 |
| identifier_str_mv | 10.1007/s10904-022-02294-0 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/21597351 |
| publishDate | 2022 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Antibacterial and Antibiofilm Activity of Mercaptophenol Functionalized-Gold Nanorods Against a Clinical Isolate of Methicillin-Resistant Staphylococcus aureusNahla O. Eltai (9291053)Nouf N. Mahmoud (9133074)Zain Z. Zakaria (6695093)Hana Abdelrahman (14151264)Ala-Eddin Al Moustafa (5472368)Maha Al-Asmakh (14151267)Macromolecular and materials chemistryMaterials engineeringMaterials ChemistryPolymers and Plastics<p>Gold nanorods (AuNRs) were synthesized by the seed-mediated wet chemical method using a binary surfactant system. AuNRs were stabilized with polyethylene glycol, then functionalized with 4-mercaptophenol (4-MPH) ligand by surface ligand exchange. The surface-functionalized AuNRs (4-MPH-AuNRs) exhibited a typical UV–vis spectrum of AuNRs with a slightly shifted longitudinal peak. Furthermore, 4-MPH-AuNRs demonstrated a similar Fourier-Transformed Infrared spectrum to 4-MPH and a fading of the thiol band, which suggests a successful functionalization through thiol-gold binding. The antibacterial and antibiofilm activities of 4-MPH-AuNRs were evaluated against a clinical isolate of Methicillin-Resistant Staphylococcus aureus (MRSA). The results indicate that 4-MPH-AuNRs exhibit a bactericidal activity with a minimum inhibitory concentration (MIC) of ~ 6.25 $$\upmu$$ μ g/mL against a planktonic suspension of MRSA. Furthermore, 4-MPH-AuNRs resulted in a 1.8–2.9 log-cycle reduction of MRSA biofilm viable count over a concentration range of 100–6.0 $$\upmu$$ μ g/mL. The bacterial uptake of the surface-modified nanorods was investigated by inductively coupled plasma-optical emission spectroscopy (ICP-OES) and scanning electron microscopy (SEM) imaging; the results reveal that the nanorods were internalized into the bacterial cells after 6 h (h) of exposure. SEM imaging revealed a significant accumulation of the nanorods at the bacterial cell wall and a possible cellular internalization. Thus, 4-MPH-AuNRs can be considered a potential antibacterial agent, particularly against MRSA strain biofilms.</p><h2>Other Information</h2> <p> Published in: Journal of Inorganic and Organometallic Polymers and Materials<br> License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="http://dx.doi.org/10.1007/s10904-022-02294-0" target="_blank">http://dx.doi.org/10.1007/s10904-022-02294-0</a></p>2022-11-22T21:13:39ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1007/s10904-022-02294-0https://figshare.com/articles/journal_contribution/Antibacterial_and_Antibiofilm_Activity_of_Mercaptophenol_Functionalized-Gold_Nanorods_Against_a_Clinical_Isolate_of_Methicillin-Resistant_Staphylococcus_aureus/21597351CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/215973512022-11-22T21:13:39Z |
| spellingShingle | Antibacterial and Antibiofilm Activity of Mercaptophenol Functionalized-Gold Nanorods Against a Clinical Isolate of Methicillin-Resistant Staphylococcus aureus Nahla O. Eltai (9291053) Macromolecular and materials chemistry Materials engineering Materials Chemistry Polymers and Plastics |
| status_str | publishedVersion |
| title | Antibacterial and Antibiofilm Activity of Mercaptophenol Functionalized-Gold Nanorods Against a Clinical Isolate of Methicillin-Resistant Staphylococcus aureus |
| title_full | Antibacterial and Antibiofilm Activity of Mercaptophenol Functionalized-Gold Nanorods Against a Clinical Isolate of Methicillin-Resistant Staphylococcus aureus |
| title_fullStr | Antibacterial and Antibiofilm Activity of Mercaptophenol Functionalized-Gold Nanorods Against a Clinical Isolate of Methicillin-Resistant Staphylococcus aureus |
| title_full_unstemmed | Antibacterial and Antibiofilm Activity of Mercaptophenol Functionalized-Gold Nanorods Against a Clinical Isolate of Methicillin-Resistant Staphylococcus aureus |
| title_short | Antibacterial and Antibiofilm Activity of Mercaptophenol Functionalized-Gold Nanorods Against a Clinical Isolate of Methicillin-Resistant Staphylococcus aureus |
| title_sort | Antibacterial and Antibiofilm Activity of Mercaptophenol Functionalized-Gold Nanorods Against a Clinical Isolate of Methicillin-Resistant Staphylococcus aureus |
| topic | Macromolecular and materials chemistry Materials engineering Materials Chemistry Polymers and Plastics |