Advances in polyhydroxyalkanoate (PHA) production from renewable waste materials using halophilic microorganisms: A comprehensive review

<p dir="ltr"><u>Polyhydroxyalkanoates </u>(PHAs) are biodegradable and biocompatible polymers that can replace conventional plastics in different sectors. However, PHA commercialization is hampered due to their high production cost resulting from the use of high purity su...

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محفوظ في:
التفاصيل البيبلوغرافية
المؤلف الرئيسي: Manel Ben Abdallah (18105505) (author)
مؤلفون آخرون: Imen Saadaoui (14159025) (author), Mohammad A. Al-Ghouti (8882054) (author), Nabil Zouari (9193418) (author), John N. Hahladakis (14151018) (author), Mohamed Chamkha (11170403) (author), Sami Sayadi (6084425) (author)
منشور في: 2025
الموضوعات:
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author Manel Ben Abdallah (18105505)
author2 Imen Saadaoui (14159025)
Mohammad A. Al-Ghouti (8882054)
Nabil Zouari (9193418)
John N. Hahladakis (14151018)
Mohamed Chamkha (11170403)
Sami Sayadi (6084425)
author2_role author
author
author
author
author
author
author_facet Manel Ben Abdallah (18105505)
Imen Saadaoui (14159025)
Mohammad A. Al-Ghouti (8882054)
Nabil Zouari (9193418)
John N. Hahladakis (14151018)
Mohamed Chamkha (11170403)
Sami Sayadi (6084425)
author_role author
dc.creator.none.fl_str_mv Manel Ben Abdallah (18105505)
Imen Saadaoui (14159025)
Mohammad A. Al-Ghouti (8882054)
Nabil Zouari (9193418)
John N. Hahladakis (14151018)
Mohamed Chamkha (11170403)
Sami Sayadi (6084425)
dc.date.none.fl_str_mv 2025-01-17T12:00:00Z
dc.identifier.none.fl_str_mv 10.1016/j.scitotenv.2025.178452
dc.relation.none.fl_str_mv https://figshare.com/articles/journal_contribution/Advances_in_polyhydroxyalkanoate_PHA_production_from_renewable_waste_materials_using_halophilic_microorganisms_A_comprehensive_review/31047580
dc.rights.none.fl_str_mv CC BY 4.0
info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Biological sciences
Genetics
Environmental sciences
Environmental biotechnology
Polyhydroxyalkanoates
Halophiles
Renewable waste materials
Advances
Cost-effective processes
Challenges
Potential applications
dc.title.none.fl_str_mv Advances in polyhydroxyalkanoate (PHA) production from renewable waste materials using halophilic microorganisms: A comprehensive review
dc.type.none.fl_str_mv Text
Journal contribution
info:eu-repo/semantics/publishedVersion
text
contribution to journal
description <p dir="ltr"><u>Polyhydroxyalkanoates </u>(PHAs) are biodegradable and biocompatible polymers that can replace conventional plastics in different sectors. However, PHA commercialization is hampered due to their high production cost resulting from the use of high purity substrates, their low conversion into PHAs by using conventional microbial chassis and the high downstream processing cost. Taking these challenges into account, researchers are focusing on the use of waste by-products as alternative low-cost <u>feedstocks</u> for fast-growing and contamination-resistant halophilic microorganisms (Bacteria, Archaea…). This is of great importance since these <u>extremophiles</u> can use low-cost substrates, produce high PHA content of <u>copolymers</u> or different PHA monomer compositions. They can present high potential for reducing the costs of PHA fermentation and recovery processes, making their use in commercial applications easier. However, little is known about the potential of <u>halophiles</u> in advancing the PHA production from renewable waste materials at lab-scale and their successful implementation at industrial-scale. This review presents actual advances in PHA production by halophilic pure/engineered species (e.g. <u>Haloferax mediterranei, Halomonas spp.</u>) and mixed <u>microbial consortia</u> (MMC) using organic waste streams. The development of optimal PHA production process involves robust genetic engineering strategies, advanced fermentation processes using mixed microbial consortia versus pure/engineered strains as well as algal biomass as feedstocks.</p><h2 dir="ltr">Other Information</h2><p dir="ltr">Published in: Science of The Total Environment<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.scitotenv.2025.178452" target="_blank">https://dx.doi.org/10.1016/j.scitotenv.2025.178452</a></p>
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identifier_str_mv 10.1016/j.scitotenv.2025.178452
network_acronym_str Manara2
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oai_identifier_str oai:figshare.com:article/31047580
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spelling Advances in polyhydroxyalkanoate (PHA) production from renewable waste materials using halophilic microorganisms: A comprehensive reviewManel Ben Abdallah (18105505)Imen Saadaoui (14159025)Mohammad A. Al-Ghouti (8882054)Nabil Zouari (9193418)John N. Hahladakis (14151018)Mohamed Chamkha (11170403)Sami Sayadi (6084425)Biological sciencesGeneticsEnvironmental sciencesEnvironmental biotechnologyPolyhydroxyalkanoatesHalophilesRenewable waste materialsAdvancesCost-effective processesChallengesPotential applications<p dir="ltr"><u>Polyhydroxyalkanoates </u>(PHAs) are biodegradable and biocompatible polymers that can replace conventional plastics in different sectors. However, PHA commercialization is hampered due to their high production cost resulting from the use of high purity substrates, their low conversion into PHAs by using conventional microbial chassis and the high downstream processing cost. Taking these challenges into account, researchers are focusing on the use of waste by-products as alternative low-cost <u>feedstocks</u> for fast-growing and contamination-resistant halophilic microorganisms (Bacteria, Archaea…). This is of great importance since these <u>extremophiles</u> can use low-cost substrates, produce high PHA content of <u>copolymers</u> or different PHA monomer compositions. They can present high potential for reducing the costs of PHA fermentation and recovery processes, making their use in commercial applications easier. However, little is known about the potential of <u>halophiles</u> in advancing the PHA production from renewable waste materials at lab-scale and their successful implementation at industrial-scale. This review presents actual advances in PHA production by halophilic pure/engineered species (e.g. <u>Haloferax mediterranei, Halomonas spp.</u>) and mixed <u>microbial consortia</u> (MMC) using organic waste streams. The development of optimal PHA production process involves robust genetic engineering strategies, advanced fermentation processes using mixed microbial consortia versus pure/engineered strains as well as algal biomass as feedstocks.</p><h2 dir="ltr">Other Information</h2><p dir="ltr">Published in: Science of The Total Environment<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.scitotenv.2025.178452" target="_blank">https://dx.doi.org/10.1016/j.scitotenv.2025.178452</a></p>2025-01-17T12:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1016/j.scitotenv.2025.178452https://figshare.com/articles/journal_contribution/Advances_in_polyhydroxyalkanoate_PHA_production_from_renewable_waste_materials_using_halophilic_microorganisms_A_comprehensive_review/31047580CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/310475802025-01-17T12:00:00Z
spellingShingle Advances in polyhydroxyalkanoate (PHA) production from renewable waste materials using halophilic microorganisms: A comprehensive review
Manel Ben Abdallah (18105505)
Biological sciences
Genetics
Environmental sciences
Environmental biotechnology
Polyhydroxyalkanoates
Halophiles
Renewable waste materials
Advances
Cost-effective processes
Challenges
Potential applications
status_str publishedVersion
title Advances in polyhydroxyalkanoate (PHA) production from renewable waste materials using halophilic microorganisms: A comprehensive review
title_full Advances in polyhydroxyalkanoate (PHA) production from renewable waste materials using halophilic microorganisms: A comprehensive review
title_fullStr Advances in polyhydroxyalkanoate (PHA) production from renewable waste materials using halophilic microorganisms: A comprehensive review
title_full_unstemmed Advances in polyhydroxyalkanoate (PHA) production from renewable waste materials using halophilic microorganisms: A comprehensive review
title_short Advances in polyhydroxyalkanoate (PHA) production from renewable waste materials using halophilic microorganisms: A comprehensive review
title_sort Advances in polyhydroxyalkanoate (PHA) production from renewable waste materials using halophilic microorganisms: A comprehensive review
topic Biological sciences
Genetics
Environmental sciences
Environmental biotechnology
Polyhydroxyalkanoates
Halophiles
Renewable waste materials
Advances
Cost-effective processes
Challenges
Potential applications