Liposomal delivery of DK14 chalcone analogue: A promising therapeutic strategy against triple-negative breast cancer
<p>Triple negative breast cancer (TNBC) is considered the most aggressive type of breast malignancy. TNBC management approaches remain limited, with a high risk of relapse and metastasis. Chalcone compounds are established for their anticancer activity. A recently patented novel chalcone compo...
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2025
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| _version_ | 1864513523968638976 |
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| author | Azza Suliman (23124484) |
| author2 | Arij Fouzat Hassan (21767516) Sumayyah Saeed (23124487) Ala-Eddin Al Moustafa (14153205) Ashraf Khalil (4591618) Abdelbary Elhissi (3397829) |
| author2_role | author author author author author |
| author_facet | Azza Suliman (23124484) Arij Fouzat Hassan (21767516) Sumayyah Saeed (23124487) Ala-Eddin Al Moustafa (14153205) Ashraf Khalil (4591618) Abdelbary Elhissi (3397829) |
| author_role | author |
| dc.creator.none.fl_str_mv | Azza Suliman (23124484) Arij Fouzat Hassan (21767516) Sumayyah Saeed (23124487) Ala-Eddin Al Moustafa (14153205) Ashraf Khalil (4591618) Abdelbary Elhissi (3397829) |
| dc.date.none.fl_str_mv | 2025-10-24T18:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1016/j.jddst.2025.107601 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Liposomal_delivery_of_DK14_chalcone_analogue_A_promising_therapeutic_strategy_against_triple-negative_breast_cancer/31239826 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Oncology and carcinogenesis Pharmacology and pharmaceutical sciences Anticancer Apoptosis Breast cancer Chalcone analogue Metastasis Liposome |
| dc.title.none.fl_str_mv | Liposomal delivery of DK14 chalcone analogue: A promising therapeutic strategy against triple-negative breast cancer |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p>Triple negative breast cancer (TNBC) is considered the most aggressive type of breast malignancy. TNBC management approaches remain limited, with a high risk of relapse and metastasis. Chalcone compounds are established for their anticancer activity. A recently patented novel chalcone compound (DK14) by our group has demonstrated a considerable activity against TNBC; however, its limited water-solubility is a challenge. In this study, we incorporated DK14 into a nano-liposomal formulation to enhance its solubility, safety and anticancer activity. The formulation was characterized in terms of particle size, zeta potential, drug entrapment, and release profile. Additionally, cytotoxicity, cell migration, colony formation and protein expression analysis were conducted. Furthermore, Angiogenesis study was performed using the chorioallantoic membrane (CAM) of the chicken embryo. DK14-liposomes demonstrated the ability to inhibit growth of TNBC cells and trigger apoptosis, accompanied by induction of BAX/BCL-2/Caspase-3 pathway. DK14-liposomes also exhibited an enhanced safety profile on non-tumorigenic mammary epithelial cells (MCF-10A) compared to free DK14 while suppressing cell migration and colony formation. Moreover, DK14-liposomes dysregulated PI3K/AKT/mTOR pathway, which is defective in TNBC; and significantly inhibited angiogenesis in ovo using a chiken embryo model. In conclusion, our results imply that DK14-liposomes have a substantial anticancer activity against TNBC with distinct anti-angiogenic properties.</p><h2>Other Information</h2> <p> Published in: Journal of Drug Delivery Science and Technology<br> License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.jddst.2025.107601" target="_blank">https://dx.doi.org/10.1016/j.jddst.2025.107601</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_a4068034d31153bd0406e62428bd1f67 |
| identifier_str_mv | 10.1016/j.jddst.2025.107601 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/31239826 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Liposomal delivery of DK14 chalcone analogue: A promising therapeutic strategy against triple-negative breast cancerAzza Suliman (23124484)Arij Fouzat Hassan (21767516)Sumayyah Saeed (23124487)Ala-Eddin Al Moustafa (14153205)Ashraf Khalil (4591618)Abdelbary Elhissi (3397829)Biomedical and clinical sciencesOncology and carcinogenesisPharmacology and pharmaceutical sciencesAnticancerApoptosisBreast cancerChalcone analogueMetastasisLiposome<p>Triple negative breast cancer (TNBC) is considered the most aggressive type of breast malignancy. TNBC management approaches remain limited, with a high risk of relapse and metastasis. Chalcone compounds are established for their anticancer activity. A recently patented novel chalcone compound (DK14) by our group has demonstrated a considerable activity against TNBC; however, its limited water-solubility is a challenge. In this study, we incorporated DK14 into a nano-liposomal formulation to enhance its solubility, safety and anticancer activity. The formulation was characterized in terms of particle size, zeta potential, drug entrapment, and release profile. Additionally, cytotoxicity, cell migration, colony formation and protein expression analysis were conducted. Furthermore, Angiogenesis study was performed using the chorioallantoic membrane (CAM) of the chicken embryo. DK14-liposomes demonstrated the ability to inhibit growth of TNBC cells and trigger apoptosis, accompanied by induction of BAX/BCL-2/Caspase-3 pathway. DK14-liposomes also exhibited an enhanced safety profile on non-tumorigenic mammary epithelial cells (MCF-10A) compared to free DK14 while suppressing cell migration and colony formation. Moreover, DK14-liposomes dysregulated PI3K/AKT/mTOR pathway, which is defective in TNBC; and significantly inhibited angiogenesis in ovo using a chiken embryo model. In conclusion, our results imply that DK14-liposomes have a substantial anticancer activity against TNBC with distinct anti-angiogenic properties.</p><h2>Other Information</h2> <p> Published in: Journal of Drug Delivery Science and Technology<br> License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.jddst.2025.107601" target="_blank">https://dx.doi.org/10.1016/j.jddst.2025.107601</a></p>2025-10-24T18:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1016/j.jddst.2025.107601https://figshare.com/articles/journal_contribution/Liposomal_delivery_of_DK14_chalcone_analogue_A_promising_therapeutic_strategy_against_triple-negative_breast_cancer/31239826CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/312398262025-10-24T18:00:00Z |
| spellingShingle | Liposomal delivery of DK14 chalcone analogue: A promising therapeutic strategy against triple-negative breast cancer Azza Suliman (23124484) Biomedical and clinical sciences Oncology and carcinogenesis Pharmacology and pharmaceutical sciences Anticancer Apoptosis Breast cancer Chalcone analogue Metastasis Liposome |
| status_str | publishedVersion |
| title | Liposomal delivery of DK14 chalcone analogue: A promising therapeutic strategy against triple-negative breast cancer |
| title_full | Liposomal delivery of DK14 chalcone analogue: A promising therapeutic strategy against triple-negative breast cancer |
| title_fullStr | Liposomal delivery of DK14 chalcone analogue: A promising therapeutic strategy against triple-negative breast cancer |
| title_full_unstemmed | Liposomal delivery of DK14 chalcone analogue: A promising therapeutic strategy against triple-negative breast cancer |
| title_short | Liposomal delivery of DK14 chalcone analogue: A promising therapeutic strategy against triple-negative breast cancer |
| title_sort | Liposomal delivery of DK14 chalcone analogue: A promising therapeutic strategy against triple-negative breast cancer |
| topic | Biomedical and clinical sciences Oncology and carcinogenesis Pharmacology and pharmaceutical sciences Anticancer Apoptosis Breast cancer Chalcone analogue Metastasis Liposome |