Blood flow restriction‐enhanced platelet‐rich plasma: A pilot randomised controlled trial protocol
<h3>Purpose</h3><p dir="ltr">To assess platelet‐rich plasma (PRP) changes in platelet and leucocyte count, insulin‐like growth factor 1 (IGF‐1), and interleukin 6 (IL‐6) concentration after bilateral low‐load knee extensions under blood flow restriction (BFR).</p>&l...
محفوظ في:
| المؤلف الرئيسي: | |
|---|---|
| مؤلفون آخرون: | , , , , |
| منشور في: |
2025
|
| الموضوعات: | |
| الوسوم: |
إضافة وسم
لا توجد وسوم, كن أول من يضع وسما على هذه التسجيلة!
|
| _version_ | 1864513538594177024 |
|---|---|
| author | Muhammad Ayyan (16328986) |
| author2 | Sushanth Alladaboina (20568785) Ayyoub Al‐Dolaymi (19730065) Mathieu Boudier‐Revéret (20568788) Emmanouil Papakostas (14070861) Theodorakys Marín Fermín (12199534) |
| author2_role | author author author author author |
| author_facet | Muhammad Ayyan (16328986) Sushanth Alladaboina (20568785) Ayyoub Al‐Dolaymi (19730065) Mathieu Boudier‐Revéret (20568788) Emmanouil Papakostas (14070861) Theodorakys Marín Fermín (12199534) |
| author_role | author |
| dc.creator.none.fl_str_mv | Muhammad Ayyan (16328986) Sushanth Alladaboina (20568785) Ayyoub Al‐Dolaymi (19730065) Mathieu Boudier‐Revéret (20568788) Emmanouil Papakostas (14070861) Theodorakys Marín Fermín (12199534) |
| dc.date.none.fl_str_mv | 2025-01-16T09:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1002/jeo2.70034 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Blood_flow_restriction_enhanced_platelet_rich_plasma_A_pilot_randomised_controlled_trial_protocol/30198043 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Clinical sciences Immunology Medical biochemistry and metabolomics Health sciences Sports science and exercise blood flow restriction injections insulin‐like growth factor 1 interleukin 6 platelet‐rich plasma |
| dc.title.none.fl_str_mv | Blood flow restriction‐enhanced platelet‐rich plasma: A pilot randomised controlled trial protocol |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <h3>Purpose</h3><p dir="ltr">To assess platelet‐rich plasma (PRP) changes in platelet and leucocyte count, insulin‐like growth factor 1 (IGF‐1), and interleukin 6 (IL‐6) concentration after bilateral low‐load knee extensions under blood flow restriction (BFR).</p><h3>Methods</h3><p dir="ltr">The present randomised controlled trial protocol will include two groups: the intervention group, which will undergo bilateral knee extensions under BFR, and the control group, which will perform bilateral knee extensions without BFR. Participants will be randomly allocated in a 1:1 ratio. Twenty‐two healthy individuals will be enrolled if the predefined inclusion criteria are met: (1) males, (2) ages 18–40, (3) Tegner activity level ≥5 and (4) with no musculoskeletal conditions that would interfere with exercise. Exclusion criteria include (1) individuals with systemic inflammatory diseases, (2) cardiovascular risk factors, (3) any blood dyscrasia, (4) Tegner Activity scale scores <5, (5) under nonsteroidal anti‐inflammatory drugs and aspirin treatment within one week before testing or (6) that had previously performed exercises on the testing day. The participant will perform low‐load bilateral knee extensions under BFR following a standard protocol of 30‐15‐15‐15 repetitions of consecutive sets with 30‐s rest intervals at 80% of limb occlusive pressure and 30% of 1‐RM load. PRP platelet and leucocyte count, IGF‐1 and IL‐6 concentration measurements (via flow cytometry, chemiluminescence testing and immunochromatography, respectively) will be conducted before exercise and 10, 20 and 30 min after the intervention.</p><h3>Results</h3><p dir="ltr">The expected outcome is that the standard protocol of low‐load bilateral knee extensions under BFR will increase the platelet and leucocyte count, IGF‐1 and IL‐6 in the PRP preparation.</p><h3>Conclusion</h3><p dir="ltr">The current protocol allows the study of an enhanced PRP formulation for its potential implementation in multiple sports injuries.</p><h2>Other Information</h2><p dir="ltr">Published in: Journal of Experimental Orthopaedics<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1002/jeo2.70034" target="_blank">https://dx.doi.org/10.1002/jeo2.70034</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_a6c1963737abb7805d9d1a602baef212 |
| identifier_str_mv | 10.1002/jeo2.70034 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/30198043 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Blood flow restriction‐enhanced platelet‐rich plasma: A pilot randomised controlled trial protocolMuhammad Ayyan (16328986)Sushanth Alladaboina (20568785)Ayyoub Al‐Dolaymi (19730065)Mathieu Boudier‐Revéret (20568788)Emmanouil Papakostas (14070861)Theodorakys Marín Fermín (12199534)Biomedical and clinical sciencesClinical sciencesImmunologyMedical biochemistry and metabolomicsHealth sciencesSports science and exerciseblood flow restrictioninjectionsinsulin‐like growth factor 1interleukin 6platelet‐rich plasma<h3>Purpose</h3><p dir="ltr">To assess platelet‐rich plasma (PRP) changes in platelet and leucocyte count, insulin‐like growth factor 1 (IGF‐1), and interleukin 6 (IL‐6) concentration after bilateral low‐load knee extensions under blood flow restriction (BFR).</p><h3>Methods</h3><p dir="ltr">The present randomised controlled trial protocol will include two groups: the intervention group, which will undergo bilateral knee extensions under BFR, and the control group, which will perform bilateral knee extensions without BFR. Participants will be randomly allocated in a 1:1 ratio. Twenty‐two healthy individuals will be enrolled if the predefined inclusion criteria are met: (1) males, (2) ages 18–40, (3) Tegner activity level ≥5 and (4) with no musculoskeletal conditions that would interfere with exercise. Exclusion criteria include (1) individuals with systemic inflammatory diseases, (2) cardiovascular risk factors, (3) any blood dyscrasia, (4) Tegner Activity scale scores <5, (5) under nonsteroidal anti‐inflammatory drugs and aspirin treatment within one week before testing or (6) that had previously performed exercises on the testing day. The participant will perform low‐load bilateral knee extensions under BFR following a standard protocol of 30‐15‐15‐15 repetitions of consecutive sets with 30‐s rest intervals at 80% of limb occlusive pressure and 30% of 1‐RM load. PRP platelet and leucocyte count, IGF‐1 and IL‐6 concentration measurements (via flow cytometry, chemiluminescence testing and immunochromatography, respectively) will be conducted before exercise and 10, 20 and 30 min after the intervention.</p><h3>Results</h3><p dir="ltr">The expected outcome is that the standard protocol of low‐load bilateral knee extensions under BFR will increase the platelet and leucocyte count, IGF‐1 and IL‐6 in the PRP preparation.</p><h3>Conclusion</h3><p dir="ltr">The current protocol allows the study of an enhanced PRP formulation for its potential implementation in multiple sports injuries.</p><h2>Other Information</h2><p dir="ltr">Published in: Journal of Experimental Orthopaedics<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1002/jeo2.70034" target="_blank">https://dx.doi.org/10.1002/jeo2.70034</a></p>2025-01-16T09:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1002/jeo2.70034https://figshare.com/articles/journal_contribution/Blood_flow_restriction_enhanced_platelet_rich_plasma_A_pilot_randomised_controlled_trial_protocol/30198043CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/301980432025-01-16T09:00:00Z |
| spellingShingle | Blood flow restriction‐enhanced platelet‐rich plasma: A pilot randomised controlled trial protocol Muhammad Ayyan (16328986) Biomedical and clinical sciences Clinical sciences Immunology Medical biochemistry and metabolomics Health sciences Sports science and exercise blood flow restriction injections insulin‐like growth factor 1 interleukin 6 platelet‐rich plasma |
| status_str | publishedVersion |
| title | Blood flow restriction‐enhanced platelet‐rich plasma: A pilot randomised controlled trial protocol |
| title_full | Blood flow restriction‐enhanced platelet‐rich plasma: A pilot randomised controlled trial protocol |
| title_fullStr | Blood flow restriction‐enhanced platelet‐rich plasma: A pilot randomised controlled trial protocol |
| title_full_unstemmed | Blood flow restriction‐enhanced platelet‐rich plasma: A pilot randomised controlled trial protocol |
| title_short | Blood flow restriction‐enhanced platelet‐rich plasma: A pilot randomised controlled trial protocol |
| title_sort | Blood flow restriction‐enhanced platelet‐rich plasma: A pilot randomised controlled trial protocol |
| topic | Biomedical and clinical sciences Clinical sciences Immunology Medical biochemistry and metabolomics Health sciences Sports science and exercise blood flow restriction injections insulin‐like growth factor 1 interleukin 6 platelet‐rich plasma |