Experimental and computational investigation of the effect of Hsc70 structural variants on inhibiting amylin aggregation
<p>The misfolding and aggregation of human islet amyloid polypeptide (hIAPP), also known as amylin, have been implicated in the pathogenesis of type 2 diabetes (T2D). Heat shock proteins, specifically, heat shock cognate 70 (Hsc70), are molecular chaperones that protect against hIAPP misfoldin...
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| مؤلفون آخرون: | , , , , |
| منشور في: |
2024
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| _version_ | 1864513516008898560 |
|---|---|
| author | Ali Chaari (827168) |
| author2 | Nabanita Saikia (1303707) Pradipta Paul (12628162) Mohammad Yousef (612992) Feng Ding (33360) Moncef Ladjimi (18560689) |
| author2_role | author author author author author |
| author_facet | Ali Chaari (827168) Nabanita Saikia (1303707) Pradipta Paul (12628162) Mohammad Yousef (612992) Feng Ding (33360) Moncef Ladjimi (18560689) |
| author_role | author |
| dc.creator.none.fl_str_mv | Ali Chaari (827168) Nabanita Saikia (1303707) Pradipta Paul (12628162) Mohammad Yousef (612992) Feng Ding (33360) Moncef Ladjimi (18560689) |
| dc.date.none.fl_str_mv | 2024-06-01T00:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1016/j.bpc.2024.107235 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Experimental_and_computational_investigation_of_the_effect_of_Hsc70_structural_variants_on_inhibiting_amylin_aggregation/25827112 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Biochemistry and cell biology Amylin Chaperone Amyloid Diabetes |
| dc.title.none.fl_str_mv | Experimental and computational investigation of the effect of Hsc70 structural variants on inhibiting amylin aggregation |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p>The misfolding and aggregation of human islet amyloid polypeptide (hIAPP), also known as amylin, have been implicated in the pathogenesis of type 2 diabetes (T2D). Heat shock proteins, specifically, heat shock cognate 70 (Hsc70), are molecular chaperones that protect against hIAPP misfolding and inhibits its aggregation. Nevertheless, there is an incomplete understanding of the mechanistic interactions between Hsc70 domains and hIAPP, thus limiting their potential therapeutic role in diabetes. This study investigates the inhibitory capacities of different Hsc70 variants, aiming to identify the structural determinants that strike a balance between efficacy and cytotoxicity. Our experimental findings demonstrate that the ATPase activity of Hsc70 is not a pivotal factor for inhibiting hIAPP misfolding. We underscore the significance of the C-terminal substrate-binding domain of Hsc70 in inhibiting hIAPP aggregation, emphasizing that the removal of the lid subdomain diminishes the inhibitory effect of Hsc70. Additionally, we employed atomistic discrete molecular dynamics simulations to gain deeper insights into the interaction between Hsc70 variants and hIAPP. Integrating both experimental and computational findings, we propose a mechanism by which Hsc70's interaction with hIAPP monomers disrupts protein-protein connections, primarily by shielding the β-sheet edges of the Hsc70-β-sandwich. The distinctive conformational dynamics of the alpha helices of Hsc70 potentially enhance hIAPP binding by obstructing the exposed edges of the β-sandwich, particularly at the β5-β8 region along the alpha helix interface. This, in turn, inhibits fibril growth, and similar results were observed following hIAPP dimerization. Overall, this study elucidates the structural intricacies of Hsc70 crucial for impeding hIAPP aggregation, improving our understanding of the potential anti-aggregative properties of molecular chaperones in diabetes treatment.</p><h2>Other Information</h2> <p> Published in: Biophysical Chemistry<br> License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.bpc.2024.107235" target="_blank">https://dx.doi.org/10.1016/j.bpc.2024.107235</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_ad5b8db97aa3b230207aa70990bcb14a |
| identifier_str_mv | 10.1016/j.bpc.2024.107235 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25827112 |
| publishDate | 2024 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Experimental and computational investigation of the effect of Hsc70 structural variants on inhibiting amylin aggregationAli Chaari (827168)Nabanita Saikia (1303707)Pradipta Paul (12628162)Mohammad Yousef (612992)Feng Ding (33360)Moncef Ladjimi (18560689)Biological sciencesBiochemistry and cell biologyAmylinChaperoneAmyloidDiabetes<p>The misfolding and aggregation of human islet amyloid polypeptide (hIAPP), also known as amylin, have been implicated in the pathogenesis of type 2 diabetes (T2D). Heat shock proteins, specifically, heat shock cognate 70 (Hsc70), are molecular chaperones that protect against hIAPP misfolding and inhibits its aggregation. Nevertheless, there is an incomplete understanding of the mechanistic interactions between Hsc70 domains and hIAPP, thus limiting their potential therapeutic role in diabetes. This study investigates the inhibitory capacities of different Hsc70 variants, aiming to identify the structural determinants that strike a balance between efficacy and cytotoxicity. Our experimental findings demonstrate that the ATPase activity of Hsc70 is not a pivotal factor for inhibiting hIAPP misfolding. We underscore the significance of the C-terminal substrate-binding domain of Hsc70 in inhibiting hIAPP aggregation, emphasizing that the removal of the lid subdomain diminishes the inhibitory effect of Hsc70. Additionally, we employed atomistic discrete molecular dynamics simulations to gain deeper insights into the interaction between Hsc70 variants and hIAPP. Integrating both experimental and computational findings, we propose a mechanism by which Hsc70's interaction with hIAPP monomers disrupts protein-protein connections, primarily by shielding the β-sheet edges of the Hsc70-β-sandwich. The distinctive conformational dynamics of the alpha helices of Hsc70 potentially enhance hIAPP binding by obstructing the exposed edges of the β-sandwich, particularly at the β5-β8 region along the alpha helix interface. This, in turn, inhibits fibril growth, and similar results were observed following hIAPP dimerization. Overall, this study elucidates the structural intricacies of Hsc70 crucial for impeding hIAPP aggregation, improving our understanding of the potential anti-aggregative properties of molecular chaperones in diabetes treatment.</p><h2>Other Information</h2> <p> Published in: Biophysical Chemistry<br> License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.bpc.2024.107235" target="_blank">https://dx.doi.org/10.1016/j.bpc.2024.107235</a></p>2024-06-01T00:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1016/j.bpc.2024.107235https://figshare.com/articles/journal_contribution/Experimental_and_computational_investigation_of_the_effect_of_Hsc70_structural_variants_on_inhibiting_amylin_aggregation/25827112CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/258271122024-06-01T00:00:00Z |
| spellingShingle | Experimental and computational investigation of the effect of Hsc70 structural variants on inhibiting amylin aggregation Ali Chaari (827168) Biological sciences Biochemistry and cell biology Amylin Chaperone Amyloid Diabetes |
| status_str | publishedVersion |
| title | Experimental and computational investigation of the effect of Hsc70 structural variants on inhibiting amylin aggregation |
| title_full | Experimental and computational investigation of the effect of Hsc70 structural variants on inhibiting amylin aggregation |
| title_fullStr | Experimental and computational investigation of the effect of Hsc70 structural variants on inhibiting amylin aggregation |
| title_full_unstemmed | Experimental and computational investigation of the effect of Hsc70 structural variants on inhibiting amylin aggregation |
| title_short | Experimental and computational investigation of the effect of Hsc70 structural variants on inhibiting amylin aggregation |
| title_sort | Experimental and computational investigation of the effect of Hsc70 structural variants on inhibiting amylin aggregation |
| topic | Biological sciences Biochemistry and cell biology Amylin Chaperone Amyloid Diabetes |