Fourier-Transform Infrared Imaging Spectroscopy and Laser Ablation -ICPMS New Vistas for Biochemical Analyses of Ischemic Stroke in Rat Brain
<h3>Objective</h3><p dir="ltr">Stroke is the main cause of adult disability in the world, leaving more than half of the patients dependent on daily assistance. Understanding the post-stroke biochemical and molecular changes are critical for patient survival and stroke man...
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2018
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| _version_ | 1864513513021505536 |
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| author | Mohamed H. M. Ali (5768066) |
| author2 | Fazle Rakib (5768069) Essam M. Abdelalim (5768072) Andreas Limbeck (4751016) Raghvendra Mall (581171) Ehsan Ullah (2698921) Nasrin Mesaeli (5768075) Donald McNaughton (5768078) Tariq Ahmed (5768081) Khalid Al-Saad (5768084) |
| author2_role | author author author author author author author author author |
| author_facet | Mohamed H. M. Ali (5768066) Fazle Rakib (5768069) Essam M. Abdelalim (5768072) Andreas Limbeck (4751016) Raghvendra Mall (581171) Ehsan Ullah (2698921) Nasrin Mesaeli (5768075) Donald McNaughton (5768078) Tariq Ahmed (5768081) Khalid Al-Saad (5768084) |
| author_role | author |
| dc.creator.none.fl_str_mv | Mohamed H. M. Ali (5768066) Fazle Rakib (5768069) Essam M. Abdelalim (5768072) Andreas Limbeck (4751016) Raghvendra Mall (581171) Ehsan Ullah (2698921) Nasrin Mesaeli (5768075) Donald McNaughton (5768078) Tariq Ahmed (5768081) Khalid Al-Saad (5768084) |
| dc.date.none.fl_str_mv | 2018-09-19T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3389/fnins.2018.00647 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Fourier-Transform_Infrared_Imaging_Spectroscopy_and_Laser_Ablation_-ICPMS_New_Vistas_for_Biochemical_Analyses_of_Ischemic_Stroke_in_Rat_Brain/25930897 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Neurosciences photothrombotic ischemic brain stroke model FTIR imaging spectroscopy LA-ICPMS lipid peroxidation neurodegeneration |
| dc.title.none.fl_str_mv | Fourier-Transform Infrared Imaging Spectroscopy and Laser Ablation -ICPMS New Vistas for Biochemical Analyses of Ischemic Stroke in Rat Brain |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <h3>Objective</h3><p dir="ltr">Stroke is the main cause of adult disability in the world, leaving more than half of the patients dependent on daily assistance. Understanding the post-stroke biochemical and molecular changes are critical for patient survival and stroke management. The aim of this work was to investigate the photo-thrombotic ischemic stroke in male rats with particular focus on biochemical and elemental changes in the primary stroke lesion in the somatosensory cortex and surrounding areas, including the corpus callosum.</p><p dir="ltr"><br></p><h3>Materials and Methods</h3><p dir="ltr">FT-IR imaging spectroscopy and LA-ICPMS techniques examined stroke brain samples, which were compared with standard immunohistochemistry studies.</p><p dir="ltr"><br></p><h3>Results</h3><p dir="ltr">The FTIR results revealed that in the lesioned gray matter the relative distribution of lipid, lipid acyl and protein contents decreased significantly. Also at this locus, there was a significant increase in aggregated protein as detected by high-levels Aβ1-42. Areas close to the stroke focus experienced decrease in the lipid and lipid acyl contents associated with an increase in lipid ester, olefin, and methyl bio-contents with a novel finding of Aβ1-42 in the PL-GM and L-WM. Elemental analyses realized major changes in the different brain structures that may underscore functionality.</p><p dir="ltr"><br></p><h3>Conclusion</h3><p dir="ltr">In conclusion, FTIR bio-spectroscopy is a non-destructive, rapid, and a refined technique to characterize oxidative stress markers associated with lipid degradation and protein denaturation not characterized by routine approaches. This technique may expedite research into stroke and offer new approaches for neurodegenerative disorders. The results suggest that a good therapeutic strategy should include a mechanism that provides protective effect from brain swelling (edema) and neurotoxicity by scavenging the lipid peroxidation end products.</p><h2>Other Information</h2><p dir="ltr">Published in: Frontiers in Neuroscience<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3389/fnins.2018.00647" target="_blank">https://dx.doi.org/10.3389/fnins.2018.00647</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_b603f39f92425841e0cbcddf04fc1297 |
| identifier_str_mv | 10.3389/fnins.2018.00647 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25930897 |
| publishDate | 2018 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Fourier-Transform Infrared Imaging Spectroscopy and Laser Ablation -ICPMS New Vistas for Biochemical Analyses of Ischemic Stroke in Rat BrainMohamed H. M. Ali (5768066)Fazle Rakib (5768069)Essam M. Abdelalim (5768072)Andreas Limbeck (4751016)Raghvendra Mall (581171)Ehsan Ullah (2698921)Nasrin Mesaeli (5768075)Donald McNaughton (5768078)Tariq Ahmed (5768081)Khalid Al-Saad (5768084)Biomedical and clinical sciencesNeurosciencesphotothromboticischemicbrainstroke modelFTIR imaging spectroscopyLA-ICPMSlipid peroxidationneurodegeneration<h3>Objective</h3><p dir="ltr">Stroke is the main cause of adult disability in the world, leaving more than half of the patients dependent on daily assistance. Understanding the post-stroke biochemical and molecular changes are critical for patient survival and stroke management. The aim of this work was to investigate the photo-thrombotic ischemic stroke in male rats with particular focus on biochemical and elemental changes in the primary stroke lesion in the somatosensory cortex and surrounding areas, including the corpus callosum.</p><p dir="ltr"><br></p><h3>Materials and Methods</h3><p dir="ltr">FT-IR imaging spectroscopy and LA-ICPMS techniques examined stroke brain samples, which were compared with standard immunohistochemistry studies.</p><p dir="ltr"><br></p><h3>Results</h3><p dir="ltr">The FTIR results revealed that in the lesioned gray matter the relative distribution of lipid, lipid acyl and protein contents decreased significantly. Also at this locus, there was a significant increase in aggregated protein as detected by high-levels Aβ1-42. Areas close to the stroke focus experienced decrease in the lipid and lipid acyl contents associated with an increase in lipid ester, olefin, and methyl bio-contents with a novel finding of Aβ1-42 in the PL-GM and L-WM. Elemental analyses realized major changes in the different brain structures that may underscore functionality.</p><p dir="ltr"><br></p><h3>Conclusion</h3><p dir="ltr">In conclusion, FTIR bio-spectroscopy is a non-destructive, rapid, and a refined technique to characterize oxidative stress markers associated with lipid degradation and protein denaturation not characterized by routine approaches. This technique may expedite research into stroke and offer new approaches for neurodegenerative disorders. The results suggest that a good therapeutic strategy should include a mechanism that provides protective effect from brain swelling (edema) and neurotoxicity by scavenging the lipid peroxidation end products.</p><h2>Other Information</h2><p dir="ltr">Published in: Frontiers in Neuroscience<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3389/fnins.2018.00647" target="_blank">https://dx.doi.org/10.3389/fnins.2018.00647</a></p>2018-09-19T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3389/fnins.2018.00647https://figshare.com/articles/journal_contribution/Fourier-Transform_Infrared_Imaging_Spectroscopy_and_Laser_Ablation_-ICPMS_New_Vistas_for_Biochemical_Analyses_of_Ischemic_Stroke_in_Rat_Brain/25930897CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/259308972018-09-19T03:00:00Z |
| spellingShingle | Fourier-Transform Infrared Imaging Spectroscopy and Laser Ablation -ICPMS New Vistas for Biochemical Analyses of Ischemic Stroke in Rat Brain Mohamed H. M. Ali (5768066) Biomedical and clinical sciences Neurosciences photothrombotic ischemic brain stroke model FTIR imaging spectroscopy LA-ICPMS lipid peroxidation neurodegeneration |
| status_str | publishedVersion |
| title | Fourier-Transform Infrared Imaging Spectroscopy and Laser Ablation -ICPMS New Vistas for Biochemical Analyses of Ischemic Stroke in Rat Brain |
| title_full | Fourier-Transform Infrared Imaging Spectroscopy and Laser Ablation -ICPMS New Vistas for Biochemical Analyses of Ischemic Stroke in Rat Brain |
| title_fullStr | Fourier-Transform Infrared Imaging Spectroscopy and Laser Ablation -ICPMS New Vistas for Biochemical Analyses of Ischemic Stroke in Rat Brain |
| title_full_unstemmed | Fourier-Transform Infrared Imaging Spectroscopy and Laser Ablation -ICPMS New Vistas for Biochemical Analyses of Ischemic Stroke in Rat Brain |
| title_short | Fourier-Transform Infrared Imaging Spectroscopy and Laser Ablation -ICPMS New Vistas for Biochemical Analyses of Ischemic Stroke in Rat Brain |
| title_sort | Fourier-Transform Infrared Imaging Spectroscopy and Laser Ablation -ICPMS New Vistas for Biochemical Analyses of Ischemic Stroke in Rat Brain |
| topic | Biomedical and clinical sciences Neurosciences photothrombotic ischemic brain stroke model FTIR imaging spectroscopy LA-ICPMS lipid peroxidation neurodegeneration |