Highly Sensitive Detection and Differentiation of Endotoxins Derived from Bacterial Pathogens by Surface-Enhanced Raman Scattering
<p dir="ltr">Bacterial endotoxins, as major components of Gram-negative bacterial outer membrane leaflets and a well-characterized TLR4-MD-2 ligand, are lipopolysaccharides (LPSs) that are constantly shed from bacteria during growth and infection. For the first time, we report that u...
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| مؤلفون آخرون: | , |
| منشور في: |
2021
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| الموضوعات: | |
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| _version_ | 1864513512242413568 |
|---|---|
| author | Xiaomeng Wu (250762) |
| author2 | Yiping Zhao (250264) Susu M. Zughaier (14151987) |
| author2_role | author author |
| author_facet | Xiaomeng Wu (250762) Yiping Zhao (250264) Susu M. Zughaier (14151987) |
| author_role | author |
| dc.creator.none.fl_str_mv | Xiaomeng Wu (250762) Yiping Zhao (250264) Susu M. Zughaier (14151987) |
| dc.date.none.fl_str_mv | 2021-07-21T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3390/bios11070234 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Highly_Sensitive_Detection_and_Differentiation_of_Endotoxins_Derived_from_Bacterial_Pathogens_by_Surface-Enhanced_Raman_Scattering/26114368 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Medical microbiology Chemical sciences Analytical chemistry Medicinal and biomolecular chemistry Engineering Nanotechnology SERS LPS bacteria endotoxin lipid A silver nanorods |
| dc.title.none.fl_str_mv | Highly Sensitive Detection and Differentiation of Endotoxins Derived from Bacterial Pathogens by Surface-Enhanced Raman Scattering |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Bacterial endotoxins, as major components of Gram-negative bacterial outer membrane leaflets and a well-characterized TLR4-MD-2 ligand, are lipopolysaccharides (LPSs) that are constantly shed from bacteria during growth and infection. For the first time, we report that unique surface-enhanced Raman scattering (SERS) spectra of enteric LPSs from E. coli, S. typhimurium, S. minnesota, V. cholerae, Rhizobium species R. CE3, and R. NGR, as well as Neisseria meningitidis endotoxin structures, LPSs, lipid A, and KDO2-lipid A can be obtained. The characteristic peaks of the SERS spectra reveal that most of the tested LPS structures are from lipids and saccharides, i.e., the major components of LPSs, and these spectra can be successfully used to differentiate between endotoxins with principal components analysis. In addition, all the LPS samples here are measured at a concentration of 10 nmole/mL, which corresponds to their relevant pathophysiological concentrations in clinical infections. This study demonstrates that LPSs can be used as biomarkers for the highly sensitive detection of bacteria using SERS-based methods.</p><h2>Other Information</h2><p dir="ltr">Published in: Biosensors<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/bios11070234" target="_blank">https://dx.doi.org/10.3390/bios11070234</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_bab64fc12ebc7cb4ec1bb8420ebbade0 |
| identifier_str_mv | 10.3390/bios11070234 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/26114368 |
| publishDate | 2021 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Highly Sensitive Detection and Differentiation of Endotoxins Derived from Bacterial Pathogens by Surface-Enhanced Raman ScatteringXiaomeng Wu (250762)Yiping Zhao (250264)Susu M. Zughaier (14151987)Biomedical and clinical sciencesMedical microbiologyChemical sciencesAnalytical chemistryMedicinal and biomolecular chemistryEngineeringNanotechnologySERSLPSbacteriaendotoxinlipid Asilver nanorods<p dir="ltr">Bacterial endotoxins, as major components of Gram-negative bacterial outer membrane leaflets and a well-characterized TLR4-MD-2 ligand, are lipopolysaccharides (LPSs) that are constantly shed from bacteria during growth and infection. For the first time, we report that unique surface-enhanced Raman scattering (SERS) spectra of enteric LPSs from E. coli, S. typhimurium, S. minnesota, V. cholerae, Rhizobium species R. CE3, and R. NGR, as well as Neisseria meningitidis endotoxin structures, LPSs, lipid A, and KDO2-lipid A can be obtained. The characteristic peaks of the SERS spectra reveal that most of the tested LPS structures are from lipids and saccharides, i.e., the major components of LPSs, and these spectra can be successfully used to differentiate between endotoxins with principal components analysis. In addition, all the LPS samples here are measured at a concentration of 10 nmole/mL, which corresponds to their relevant pathophysiological concentrations in clinical infections. This study demonstrates that LPSs can be used as biomarkers for the highly sensitive detection of bacteria using SERS-based methods.</p><h2>Other Information</h2><p dir="ltr">Published in: Biosensors<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/bios11070234" target="_blank">https://dx.doi.org/10.3390/bios11070234</a></p>2021-07-21T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3390/bios11070234https://figshare.com/articles/journal_contribution/Highly_Sensitive_Detection_and_Differentiation_of_Endotoxins_Derived_from_Bacterial_Pathogens_by_Surface-Enhanced_Raman_Scattering/26114368CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/261143682021-07-21T03:00:00Z |
| spellingShingle | Highly Sensitive Detection and Differentiation of Endotoxins Derived from Bacterial Pathogens by Surface-Enhanced Raman Scattering Xiaomeng Wu (250762) Biomedical and clinical sciences Medical microbiology Chemical sciences Analytical chemistry Medicinal and biomolecular chemistry Engineering Nanotechnology SERS LPS bacteria endotoxin lipid A silver nanorods |
| status_str | publishedVersion |
| title | Highly Sensitive Detection and Differentiation of Endotoxins Derived from Bacterial Pathogens by Surface-Enhanced Raman Scattering |
| title_full | Highly Sensitive Detection and Differentiation of Endotoxins Derived from Bacterial Pathogens by Surface-Enhanced Raman Scattering |
| title_fullStr | Highly Sensitive Detection and Differentiation of Endotoxins Derived from Bacterial Pathogens by Surface-Enhanced Raman Scattering |
| title_full_unstemmed | Highly Sensitive Detection and Differentiation of Endotoxins Derived from Bacterial Pathogens by Surface-Enhanced Raman Scattering |
| title_short | Highly Sensitive Detection and Differentiation of Endotoxins Derived from Bacterial Pathogens by Surface-Enhanced Raman Scattering |
| title_sort | Highly Sensitive Detection and Differentiation of Endotoxins Derived from Bacterial Pathogens by Surface-Enhanced Raman Scattering |
| topic | Biomedical and clinical sciences Medical microbiology Chemical sciences Analytical chemistry Medicinal and biomolecular chemistry Engineering Nanotechnology SERS LPS bacteria endotoxin lipid A silver nanorods |