In vitro Interleukin-7 treatment partially rescues MAIT cell dysfunction caused by SARS-CoV-2 infection
<div><p>MAIT cells have been shown to be activated upon several viral infections in a TCR-independent manner by responding to inflammatory cytokines secreted by antigen-presenting cells. Recently, a few studies have shown a similar activation of MAIT cells in response to severe acute res...
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2021
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| _version_ | 1864513515354587136 |
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| author | Satanay Hubrack (194364) |
| author2 | Maryam Ali Al-Nesf (13040248) Nourhen Agrebi (14151222) Christophe Raynaud (1405585) Mohammed Abu Khattab (18589408) Merlin Thomas (6252059) Tayseer Ibrahim (18589411) Salma Taha (18589414) Said Dermime (79420) Maysaloun Merhi (4246147) Michal Kulinski (3520070) Martin Steinhoff (5340194) Patrick Tang (239534) Bernice Lo (3441317) |
| author2_role | author author author author author author author author author author author author author |
| author_facet | Satanay Hubrack (194364) Maryam Ali Al-Nesf (13040248) Nourhen Agrebi (14151222) Christophe Raynaud (1405585) Mohammed Abu Khattab (18589408) Merlin Thomas (6252059) Tayseer Ibrahim (18589411) Salma Taha (18589414) Said Dermime (79420) Maysaloun Merhi (4246147) Michal Kulinski (3520070) Martin Steinhoff (5340194) Patrick Tang (239534) Bernice Lo (3441317) |
| author_role | author |
| dc.creator.none.fl_str_mv | Satanay Hubrack (194364) Maryam Ali Al-Nesf (13040248) Nourhen Agrebi (14151222) Christophe Raynaud (1405585) Mohammed Abu Khattab (18589408) Merlin Thomas (6252059) Tayseer Ibrahim (18589411) Salma Taha (18589414) Said Dermime (79420) Maysaloun Merhi (4246147) Michal Kulinski (3520070) Martin Steinhoff (5340194) Patrick Tang (239534) Bernice Lo (3441317) |
| dc.date.none.fl_str_mv | 2021-07-08T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1038/s41598-021-93536-7 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/In_vitro_Interleukin-7_treatment_partially_rescues_MAIT_cell_dysfunction_caused_by_SARS-CoV-2_infection/25867657 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Microbiology Biomedical and clinical sciences Immunology Phenotype In vitro stimulation Antiviral function Decline Hospitalized donors Healthy donors TNF-alpha Perforin |
| dc.title.none.fl_str_mv | In vitro Interleukin-7 treatment partially rescues MAIT cell dysfunction caused by SARS-CoV-2 infection |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <div><p>MAIT cells have been shown to be activated upon several viral infections in a TCR-independent manner by responding to inflammatory cytokines secreted by antigen-presenting cells. Recently, a few studies have shown a similar activation of MAIT cells in response to severe acute respiratory coronavirus 2 (SARS-CoV-2) infection. In this study, we investigate the effect of SARS-CoV-2 infection on the frequency and phenotype of MAIT cells by flow cytometry, and we test in vitro stimulation conditions on the capacity to enhance or rescue the antiviral function of MAIT cells from patients with coronavirus disease 2019 (COVID-19). Our study, in agreement with recently published studies, confirmed the decline in MAIT cell frequency of hospitalized donors in comparison to healthy donors. MAIT cells of COVID-19 patients also had lower expression levels of TNF-alpha, perforin and granzyme B upon stimulation with IL-12 + IL-18. 24 h’ incubation with IL-7 successfully restored perforin expression levels in COVID-19 patients. Combined, our findings support the growing evidence that SARS-CoV-2 is dysregulating MAIT cells and that IL-7 treatment might improve their function, rendering them more effective in protecting the body against the virus.</p><p> </p></div><h2>Other Information</h2> <p> Published in: Scientific Reports<br> License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1038/s41598-021-93536-7" target="_blank">https://dx.doi.org/10.1038/s41598-021-93536-7</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_bad4446b8b9f3ce77e7fd382cb71ae6a |
| identifier_str_mv | 10.1038/s41598-021-93536-7 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25867657 |
| publishDate | 2021 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | In vitro Interleukin-7 treatment partially rescues MAIT cell dysfunction caused by SARS-CoV-2 infectionSatanay Hubrack (194364)Maryam Ali Al-Nesf (13040248)Nourhen Agrebi (14151222)Christophe Raynaud (1405585)Mohammed Abu Khattab (18589408)Merlin Thomas (6252059)Tayseer Ibrahim (18589411)Salma Taha (18589414)Said Dermime (79420)Maysaloun Merhi (4246147)Michal Kulinski (3520070)Martin Steinhoff (5340194)Patrick Tang (239534)Bernice Lo (3441317)Biological sciencesMicrobiologyBiomedical and clinical sciencesImmunologyPhenotypeIn vitro stimulationAntiviral functionDeclineHospitalized donorsHealthy donorsTNF-alphaPerforin<div><p>MAIT cells have been shown to be activated upon several viral infections in a TCR-independent manner by responding to inflammatory cytokines secreted by antigen-presenting cells. Recently, a few studies have shown a similar activation of MAIT cells in response to severe acute respiratory coronavirus 2 (SARS-CoV-2) infection. In this study, we investigate the effect of SARS-CoV-2 infection on the frequency and phenotype of MAIT cells by flow cytometry, and we test in vitro stimulation conditions on the capacity to enhance or rescue the antiviral function of MAIT cells from patients with coronavirus disease 2019 (COVID-19). Our study, in agreement with recently published studies, confirmed the decline in MAIT cell frequency of hospitalized donors in comparison to healthy donors. MAIT cells of COVID-19 patients also had lower expression levels of TNF-alpha, perforin and granzyme B upon stimulation with IL-12 + IL-18. 24 h’ incubation with IL-7 successfully restored perforin expression levels in COVID-19 patients. Combined, our findings support the growing evidence that SARS-CoV-2 is dysregulating MAIT cells and that IL-7 treatment might improve their function, rendering them more effective in protecting the body against the virus.</p><p> </p></div><h2>Other Information</h2> <p> Published in: Scientific Reports<br> License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1038/s41598-021-93536-7" target="_blank">https://dx.doi.org/10.1038/s41598-021-93536-7</a></p>2021-07-08T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1038/s41598-021-93536-7https://figshare.com/articles/journal_contribution/In_vitro_Interleukin-7_treatment_partially_rescues_MAIT_cell_dysfunction_caused_by_SARS-CoV-2_infection/25867657CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/258676572021-07-08T03:00:00Z |
| spellingShingle | In vitro Interleukin-7 treatment partially rescues MAIT cell dysfunction caused by SARS-CoV-2 infection Satanay Hubrack (194364) Biological sciences Microbiology Biomedical and clinical sciences Immunology Phenotype In vitro stimulation Antiviral function Decline Hospitalized donors Healthy donors TNF-alpha Perforin |
| status_str | publishedVersion |
| title | In vitro Interleukin-7 treatment partially rescues MAIT cell dysfunction caused by SARS-CoV-2 infection |
| title_full | In vitro Interleukin-7 treatment partially rescues MAIT cell dysfunction caused by SARS-CoV-2 infection |
| title_fullStr | In vitro Interleukin-7 treatment partially rescues MAIT cell dysfunction caused by SARS-CoV-2 infection |
| title_full_unstemmed | In vitro Interleukin-7 treatment partially rescues MAIT cell dysfunction caused by SARS-CoV-2 infection |
| title_short | In vitro Interleukin-7 treatment partially rescues MAIT cell dysfunction caused by SARS-CoV-2 infection |
| title_sort | In vitro Interleukin-7 treatment partially rescues MAIT cell dysfunction caused by SARS-CoV-2 infection |
| topic | Biological sciences Microbiology Biomedical and clinical sciences Immunology Phenotype In vitro stimulation Antiviral function Decline Hospitalized donors Healthy donors TNF-alpha Perforin |