Investigation and application of Bacillus pumilus QBP344-3 in the control of Aspergillus carbonarius and ochratoxin A contamination
<p dir="ltr">This study was designed to investigate the antifungal activity of <i>Bacillus pumilus</i> 344-3 against toxigenic fungi. <i>In vitro</i> co-incubation assay revealed that <i>A. carbonarius</i> AC82, <i>A. niger</i> AN8 and...
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | , , , , |
| Published: |
2021
|
| Subjects: | |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | <p dir="ltr">This study was designed to investigate the antifungal activity of <i>Bacillus pumilus</i> 344-3 against toxigenic fungi. <i>In vitro</i> co-incubation assay revealed that <i>A. carbonarius</i> AC82, <i>A. niger</i> AN8 and <i>P. digitatum</i> PD43 are most sensitive fungi to bacterial antifungal compounds with zone of inhibition of 29.2 mm, 27.7 mm and 27.1 mm, respectively. The addition of <i>Bacillus pumilus</i> 344-3 culture supernatant at low concentration in the fungal growth medium stimulated <i>A. carbonarius</i> biomass, but inhibited ochratoxin A (OTA) synthesis significantly (<i>p</i> ≤ 0.05). Conidial germination of <i>A. carbonarius</i> was not affected in a medium containing 10% and 20% of the bacterial culture supernatant, while it was completely inhibited in 100% bacterial extract. Storage of bacterial culture supernatant at temperature ranging from −20 °C to 100 °C for 1 h, didn't affect its antifungal potential. <i>In vivo</i> application of bacterial extract on the maize kernels, showed 95% protection against <i>A. carbonarius</i> infection. Application of <i>B. pumilus</i> 344-3 culture supernatant on the surface of maize kernels provided 99% reduction in OTA production potential of <i>A. carbonarius</i> AC82. Because of its strong activities against the growth of <i>A. carbonarius</i> AC82 and OTA-synthesis, <i>B. pumilus</i> 344-3 can be considered as a very promising biocontrol agent.</p><h2>Other Information</h2><p dir="ltr">Published in: Food Control<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.foodcont.2020.107464" target="_blank">https://dx.doi.org/10.1016/j.foodcont.2020.107464</a></p> |
|---|