Investigation and application of Bacillus pumilus QBP344-3 in the control of Aspergillus carbonarius and ochratoxin A contamination
<p dir="ltr">This study was designed to investigate the antifungal activity of <i>Bacillus pumilus</i> 344-3 against toxigenic fungi. <i>In vitro</i> co-incubation assay revealed that <i>A. carbonarius</i> AC82, <i>A. niger</i> AN8 and...
محفوظ في:
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| مؤلفون آخرون: | , , , , |
| منشور في: |
2021
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إضافة وسم
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| _version_ | 1864513555068354560 |
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| author | Nayla Salah Higazy (17092927) |
| author2 | Aya Ehab Saleh (17092930) Zahoor Ul Hassan (9759642) Roda Al Thani (17092933) Quirico Migheli (381438) Samir Jaoua (11624805) |
| author2_role | author author author author author |
| author_facet | Nayla Salah Higazy (17092927) Aya Ehab Saleh (17092930) Zahoor Ul Hassan (9759642) Roda Al Thani (17092933) Quirico Migheli (381438) Samir Jaoua (11624805) |
| author_role | author |
| dc.creator.none.fl_str_mv | Nayla Salah Higazy (17092927) Aya Ehab Saleh (17092930) Zahoor Ul Hassan (9759642) Roda Al Thani (17092933) Quirico Migheli (381438) Samir Jaoua (11624805) |
| dc.date.none.fl_str_mv | 2021-01-01T00:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1016/j.foodcont.2020.107464 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Investigation_and_application_of_Bacillus_pumilus_QBP344-3_in_the_control_of_Aspergillus_carbonarius_and_ochratoxin_A_contamination/24242500 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Agricultural, veterinary and food sciences Food sciences Environmental sciences Environmental biotechnology Bacillus pumilus Antifungal activity Mycotoxins A. carbonarius Maize |
| dc.title.none.fl_str_mv | Investigation and application of Bacillus pumilus QBP344-3 in the control of Aspergillus carbonarius and ochratoxin A contamination |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">This study was designed to investigate the antifungal activity of <i>Bacillus pumilus</i> 344-3 against toxigenic fungi. <i>In vitro</i> co-incubation assay revealed that <i>A. carbonarius</i> AC82, <i>A. niger</i> AN8 and <i>P. digitatum</i> PD43 are most sensitive fungi to bacterial antifungal compounds with zone of inhibition of 29.2 mm, 27.7 mm and 27.1 mm, respectively. The addition of <i>Bacillus pumilus</i> 344-3 culture supernatant at low concentration in the fungal growth medium stimulated <i>A. carbonarius</i> biomass, but inhibited ochratoxin A (OTA) synthesis significantly (<i>p</i> ≤ 0.05). Conidial germination of <i>A. carbonarius</i> was not affected in a medium containing 10% and 20% of the bacterial culture supernatant, while it was completely inhibited in 100% bacterial extract. Storage of bacterial culture supernatant at temperature ranging from −20 °C to 100 °C for 1 h, didn't affect its antifungal potential. <i>In vivo</i> application of bacterial extract on the maize kernels, showed 95% protection against <i>A. carbonarius</i> infection. Application of <i>B. pumilus</i> 344-3 culture supernatant on the surface of maize kernels provided 99% reduction in OTA production potential of <i>A. carbonarius</i> AC82. Because of its strong activities against the growth of <i>A. carbonarius</i> AC82 and OTA-synthesis, <i>B. pumilus</i> 344-3 can be considered as a very promising biocontrol agent.</p><h2>Other Information</h2><p dir="ltr">Published in: Food Control<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.foodcont.2020.107464" target="_blank">https://dx.doi.org/10.1016/j.foodcont.2020.107464</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_bcf2e17ec025f61ac72c930ef0605d5a |
| identifier_str_mv | 10.1016/j.foodcont.2020.107464 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/24242500 |
| publishDate | 2021 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Investigation and application of Bacillus pumilus QBP344-3 in the control of Aspergillus carbonarius and ochratoxin A contaminationNayla Salah Higazy (17092927)Aya Ehab Saleh (17092930)Zahoor Ul Hassan (9759642)Roda Al Thani (17092933)Quirico Migheli (381438)Samir Jaoua (11624805)Agricultural, veterinary and food sciencesFood sciencesEnvironmental sciencesEnvironmental biotechnologyBacillus pumilusAntifungal activityMycotoxinsA. carbonariusMaize<p dir="ltr">This study was designed to investigate the antifungal activity of <i>Bacillus pumilus</i> 344-3 against toxigenic fungi. <i>In vitro</i> co-incubation assay revealed that <i>A. carbonarius</i> AC82, <i>A. niger</i> AN8 and <i>P. digitatum</i> PD43 are most sensitive fungi to bacterial antifungal compounds with zone of inhibition of 29.2 mm, 27.7 mm and 27.1 mm, respectively. The addition of <i>Bacillus pumilus</i> 344-3 culture supernatant at low concentration in the fungal growth medium stimulated <i>A. carbonarius</i> biomass, but inhibited ochratoxin A (OTA) synthesis significantly (<i>p</i> ≤ 0.05). Conidial germination of <i>A. carbonarius</i> was not affected in a medium containing 10% and 20% of the bacterial culture supernatant, while it was completely inhibited in 100% bacterial extract. Storage of bacterial culture supernatant at temperature ranging from −20 °C to 100 °C for 1 h, didn't affect its antifungal potential. <i>In vivo</i> application of bacterial extract on the maize kernels, showed 95% protection against <i>A. carbonarius</i> infection. Application of <i>B. pumilus</i> 344-3 culture supernatant on the surface of maize kernels provided 99% reduction in OTA production potential of <i>A. carbonarius</i> AC82. Because of its strong activities against the growth of <i>A. carbonarius</i> AC82 and OTA-synthesis, <i>B. pumilus</i> 344-3 can be considered as a very promising biocontrol agent.</p><h2>Other Information</h2><p dir="ltr">Published in: Food Control<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.foodcont.2020.107464" target="_blank">https://dx.doi.org/10.1016/j.foodcont.2020.107464</a></p>2021-01-01T00:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1016/j.foodcont.2020.107464https://figshare.com/articles/journal_contribution/Investigation_and_application_of_Bacillus_pumilus_QBP344-3_in_the_control_of_Aspergillus_carbonarius_and_ochratoxin_A_contamination/24242500CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/242425002021-01-01T00:00:00Z |
| spellingShingle | Investigation and application of Bacillus pumilus QBP344-3 in the control of Aspergillus carbonarius and ochratoxin A contamination Nayla Salah Higazy (17092927) Agricultural, veterinary and food sciences Food sciences Environmental sciences Environmental biotechnology Bacillus pumilus Antifungal activity Mycotoxins A. carbonarius Maize |
| status_str | publishedVersion |
| title | Investigation and application of Bacillus pumilus QBP344-3 in the control of Aspergillus carbonarius and ochratoxin A contamination |
| title_full | Investigation and application of Bacillus pumilus QBP344-3 in the control of Aspergillus carbonarius and ochratoxin A contamination |
| title_fullStr | Investigation and application of Bacillus pumilus QBP344-3 in the control of Aspergillus carbonarius and ochratoxin A contamination |
| title_full_unstemmed | Investigation and application of Bacillus pumilus QBP344-3 in the control of Aspergillus carbonarius and ochratoxin A contamination |
| title_short | Investigation and application of Bacillus pumilus QBP344-3 in the control of Aspergillus carbonarius and ochratoxin A contamination |
| title_sort | Investigation and application of Bacillus pumilus QBP344-3 in the control of Aspergillus carbonarius and ochratoxin A contamination |
| topic | Agricultural, veterinary and food sciences Food sciences Environmental sciences Environmental biotechnology Bacillus pumilus Antifungal activity Mycotoxins A. carbonarius Maize |