Interactions of ultrashort laser pulses with hemoglobin: Photophysical aspects and potential applications
<p>Hemoglobin (Hb), a life-sustaining and highly abundant erythrocyte protein, is not readily fluorescent. A few studies have already reported Two-Photon Excited Fluorescence (TPEF) of Hb, however, the mechanisms through which Hb becomes fluorescent upon interaction with ultrashort laser pulse...
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | , , , , , , , , |
| Published: |
2023
|
| Subjects: | |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1864513562888634368 |
|---|---|
| author | Mihajlo D. Radmilović (16538659) |
| author2 | Ivana T. Drvenica (16538660) Mihailo D. Rabasović (16538661) Vesna Lj. Ilić (16538664) Danica Pavlović (16538667) Sho Oasa (7244462) Vladana Vukojević (165297) Mina Perić (8859218) Stanko N. Nikolić (16538668) Aleksandar J. Krmpot (16538671) |
| author2_role | author author author author author author author author author |
| author_facet | Mihajlo D. Radmilović (16538659) Ivana T. Drvenica (16538660) Mihailo D. Rabasović (16538661) Vesna Lj. Ilić (16538664) Danica Pavlović (16538667) Sho Oasa (7244462) Vladana Vukojević (165297) Mina Perić (8859218) Stanko N. Nikolić (16538668) Aleksandar J. Krmpot (16538671) |
| author_role | author |
| dc.creator.none.fl_str_mv | Mihajlo D. Radmilović (16538659) Ivana T. Drvenica (16538660) Mihailo D. Rabasović (16538661) Vesna Lj. Ilić (16538664) Danica Pavlović (16538667) Sho Oasa (7244462) Vladana Vukojević (165297) Mina Perić (8859218) Stanko N. Nikolić (16538668) Aleksandar J. Krmpot (16538671) |
| dc.date.none.fl_str_mv | 2023-07-31T00:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1016/j.ijbiomac.2023.125312 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Interactions_of_ultrashort_laser_pulses_with_hemoglobin_Photophysical_aspects_and_potential_applications/23674215 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Cardiovascular medicine and haematology Erythrocytes Two-photon excitation fluorescence Hemoglobin photoproduct Femtosecond laser Protoporphyrin IX |
| dc.title.none.fl_str_mv | Interactions of ultrashort laser pulses with hemoglobin: Photophysical aspects and potential applications |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p>Hemoglobin (Hb), a life-sustaining and highly abundant erythrocyte protein, is not readily fluorescent. A few studies have already reported Two-Photon Excited Fluorescence (TPEF) of Hb, however, the mechanisms through which Hb becomes fluorescent upon interaction with ultrashort laser pulses are not completely understood. Here, we characterized photophysically this interaction on Hb thin film and erythrocytes using fluorescence spectroscopy upon single-photon/two-photon absorption, and UV-VIS single-photon absorption spectroscopy. A gradual increase of the fluorescence intensity, ending up with saturation, is observed upon prolonged exposure of Hb thin layer and erythrocytes to ultrashort laser pulses at 730 nm. When compared to protoporphyrin IX (PpIX) and oxidized Hb by H<sub>2</sub>O<sub>2</sub>, TPEF spectra from a thin Hb film and erythrocytes showed good mutual agreement, broad peaking at 550 nm, supporting hemoglobin undergoes degradation and that same fluorescent specie(s) originating from the heme moiety are generated. The uniform square shaped patterns of the fluorescent photoproduct exhibited the same level of the fluorescence intensity even after 12 weeks from the formation, indicating high photoproduct stability. We finally demonstrated the full potential of the formed Hb photoproduct with TPEF scanning microscopy towards spatiotemporally controlled micropatterning in HTF and single human erythrocyte labelling and tracking in the whole blood. </p> <h2>Other Information</h2> <p>Published in: International Journal of Biological Macromolecules<br> License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br> See article on publisher's website: <a href="http://dx.doi.org/10.1016/j.ijbiomac.2023.125312" target="_blank">http://dx.doi.org/10.1016/j.ijbiomac.2023.125312 </a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_c541522e2b228040cb35e9b568cab0f2 |
| identifier_str_mv | 10.1016/j.ijbiomac.2023.125312 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/23674215 |
| publishDate | 2023 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Interactions of ultrashort laser pulses with hemoglobin: Photophysical aspects and potential applicationsMihajlo D. Radmilović (16538659)Ivana T. Drvenica (16538660)Mihailo D. Rabasović (16538661)Vesna Lj. Ilić (16538664)Danica Pavlović (16538667)Sho Oasa (7244462)Vladana Vukojević (165297)Mina Perić (8859218)Stanko N. Nikolić (16538668)Aleksandar J. Krmpot (16538671)Biological sciencesBiochemistry and cell biologyBiomedical and clinical sciencesCardiovascular medicine and haematologyErythrocytesTwo-photon excitation fluorescenceHemoglobin photoproductFemtosecond laserProtoporphyrin IX<p>Hemoglobin (Hb), a life-sustaining and highly abundant erythrocyte protein, is not readily fluorescent. A few studies have already reported Two-Photon Excited Fluorescence (TPEF) of Hb, however, the mechanisms through which Hb becomes fluorescent upon interaction with ultrashort laser pulses are not completely understood. Here, we characterized photophysically this interaction on Hb thin film and erythrocytes using fluorescence spectroscopy upon single-photon/two-photon absorption, and UV-VIS single-photon absorption spectroscopy. A gradual increase of the fluorescence intensity, ending up with saturation, is observed upon prolonged exposure of Hb thin layer and erythrocytes to ultrashort laser pulses at 730 nm. When compared to protoporphyrin IX (PpIX) and oxidized Hb by H<sub>2</sub>O<sub>2</sub>, TPEF spectra from a thin Hb film and erythrocytes showed good mutual agreement, broad peaking at 550 nm, supporting hemoglobin undergoes degradation and that same fluorescent specie(s) originating from the heme moiety are generated. The uniform square shaped patterns of the fluorescent photoproduct exhibited the same level of the fluorescence intensity even after 12 weeks from the formation, indicating high photoproduct stability. We finally demonstrated the full potential of the formed Hb photoproduct with TPEF scanning microscopy towards spatiotemporally controlled micropatterning in HTF and single human erythrocyte labelling and tracking in the whole blood. </p> <h2>Other Information</h2> <p>Published in: International Journal of Biological Macromolecules<br> License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br> See article on publisher's website: <a href="http://dx.doi.org/10.1016/j.ijbiomac.2023.125312" target="_blank">http://dx.doi.org/10.1016/j.ijbiomac.2023.125312 </a></p>2023-07-31T00:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1016/j.ijbiomac.2023.125312https://figshare.com/articles/journal_contribution/Interactions_of_ultrashort_laser_pulses_with_hemoglobin_Photophysical_aspects_and_potential_applications/23674215CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/236742152023-07-31T00:00:00Z |
| spellingShingle | Interactions of ultrashort laser pulses with hemoglobin: Photophysical aspects and potential applications Mihajlo D. Radmilović (16538659) Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Cardiovascular medicine and haematology Erythrocytes Two-photon excitation fluorescence Hemoglobin photoproduct Femtosecond laser Protoporphyrin IX |
| status_str | publishedVersion |
| title | Interactions of ultrashort laser pulses with hemoglobin: Photophysical aspects and potential applications |
| title_full | Interactions of ultrashort laser pulses with hemoglobin: Photophysical aspects and potential applications |
| title_fullStr | Interactions of ultrashort laser pulses with hemoglobin: Photophysical aspects and potential applications |
| title_full_unstemmed | Interactions of ultrashort laser pulses with hemoglobin: Photophysical aspects and potential applications |
| title_short | Interactions of ultrashort laser pulses with hemoglobin: Photophysical aspects and potential applications |
| title_sort | Interactions of ultrashort laser pulses with hemoglobin: Photophysical aspects and potential applications |
| topic | Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Cardiovascular medicine and haematology Erythrocytes Two-photon excitation fluorescence Hemoglobin photoproduct Femtosecond laser Protoporphyrin IX |