The Immunophenotyping Changes of Peripheral CD4+ T Lymphocytes and Inflammatory Markers of Class III Obesity Subjects After Laparoscopic Gastric Sleeve Surgery – A Follow-Up Study
<h3>Purpose </h3><p dir="ltr">Obesity is a chronic disorder characterized by a low-grade inflammatory state and immune cell irregularities. The study aimed to follow up on the changes in the peripheral CD4+ T lymphocytes and the pro-inflammatory cytokines; IL-6, TNF-alpha...
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| مؤلفون آخرون: | , , , |
| منشور في: |
2021
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| _version_ | 1864513511788380160 |
|---|---|
| author | Nasser M Rizk (18810328) |
| author2 | Amina Fadel (18810331) Wasaif AlShammari (18810334) Noura Younes (2029393) Moataz Bashah (14148867) |
| author2_role | author author author author |
| author_facet | Nasser M Rizk (18810328) Amina Fadel (18810331) Wasaif AlShammari (18810334) Noura Younes (2029393) Moataz Bashah (14148867) |
| author_role | author |
| dc.creator.none.fl_str_mv | Nasser M Rizk (18810328) Amina Fadel (18810331) Wasaif AlShammari (18810334) Noura Younes (2029393) Moataz Bashah (14148867) |
| dc.date.none.fl_str_mv | 2021-05-01T00:00:00Z |
| dc.identifier.none.fl_str_mv | 10.2147/jir.s282189 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/The_Immunophenotyping_Changes_of_Peripheral_CD4_T_Lymphocytes_and_Inflammatory_Markers_of_Class_III_Obesity_Subjects_After_Laparoscopic_Gastric_Sleeve_Surgery_A_Follow-Up_Study/26020930 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Clinical sciences Immunology class III obesity immunity peripheral CD4+ T-lymphocytes T-regulatory cells follow up laparoscope gastric sleeve surgery |
| dc.title.none.fl_str_mv | The Immunophenotyping Changes of Peripheral CD4+ T Lymphocytes and Inflammatory Markers of Class III Obesity Subjects After Laparoscopic Gastric Sleeve Surgery – A Follow-Up Study |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <h3>Purpose </h3><p dir="ltr">Obesity is a chronic disorder characterized by a low-grade inflammatory state and immune cell irregularities. The study aimed to follow up on the changes in the peripheral CD4+ T lymphocytes and the pro-inflammatory cytokines; IL-6, TNF-alpha, MCP-1, and IL-10 at baseline and 12 weeks post-surgical intervention by the laparoscopic gastric sleeve (LGS) in morbidly obese patients (class III obesity subjects). </p><h3>Materials and Methods </h3><p dir="ltr">A prospective longitudinal research included 24 class III obesity subjects with a BMI > 40 kg/m<sup>2</sup>. The subjects were enrolled from the Metabolic/Surgical Department at Hamad Medical Corporation (HMC)-Qatar. Fasting blood samples were collected at admission to LGS for weight loss and after 12 weeks of LGS. The immunophenotype of CD4+ T-cell populations; naïve (CD45RA<sup>+</sup>and CD27<sup>+</sup>), central memory T cells (CD45RO<sup>+</sup> and CD27<sup>+</sup>), and effector memory (CD45RO<sup>+</sup>and CD27<sup>−</sup>) and T-regulatory cell (CD4<sup>+</sup>CD25<sup>+</sup> FoxP3<sup>+</sup>) were identified using flow cytometry. Plasma pro-inflammatory cytokines and adipokines were evaluated. A control group of lean subjects was used to compare changes of T-regulatory and inflammatory biomarkers with postoperative changes in obese patients. </p><h3>Results </h3><p dir="ltr">The means (SD) of age and BMI of class III obesity subjects was 32.32 (8.36) years and 49.02 (6.28) kg/m<sup>2</sup>, respectively. LGS caused a significant reduction in BMI by 32%, p< 0.0001. LGS intervention significantly decreased CD4+ T-lymphocytes and effector memory (TEM) cells but increased T-regulatory (Treg), naïve, and central memory (TCM) cells, with all p values < 0.05. The increase of Treg cells postoperative is significantly lower compared to lean subjects, p < 0.05. A significant reduction of plasma IL-6, TNF-α, and MCP-1, but IL-10 significantly increased after LGS, with all p< 0.05. Adiponectin/leptin ratio improved after LGS by 2.9 folds, p< 0.0001. </p><h3>Conclusion </h3><p dir="ltr">Weight loss by LGS accomplished a substantial rise of Treg and decreased EM T-lymphocytes with a shift from pro-inflammatory to the anti-inflammatory pattern.</p><h2>Other Information</h2><p dir="ltr">Published in: Journal of Inflammation Research<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.2147/jir.s282189" target="_blank">https://dx.doi.org/10.2147/jir.s282189</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_c9014efe39521af2891419cbcba38fa4 |
| identifier_str_mv | 10.2147/jir.s282189 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/26020930 |
| publishDate | 2021 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | The Immunophenotyping Changes of Peripheral CD4+ T Lymphocytes and Inflammatory Markers of Class III Obesity Subjects After Laparoscopic Gastric Sleeve Surgery – A Follow-Up StudyNasser M Rizk (18810328)Amina Fadel (18810331)Wasaif AlShammari (18810334)Noura Younes (2029393)Moataz Bashah (14148867)Biomedical and clinical sciencesClinical sciencesImmunologyclass III obesityimmunityperipheral CD4+ T-lymphocytesT-regulatory cellsfollow uplaparoscope gastric sleeve surgery<h3>Purpose </h3><p dir="ltr">Obesity is a chronic disorder characterized by a low-grade inflammatory state and immune cell irregularities. The study aimed to follow up on the changes in the peripheral CD4+ T lymphocytes and the pro-inflammatory cytokines; IL-6, TNF-alpha, MCP-1, and IL-10 at baseline and 12 weeks post-surgical intervention by the laparoscopic gastric sleeve (LGS) in morbidly obese patients (class III obesity subjects). </p><h3>Materials and Methods </h3><p dir="ltr">A prospective longitudinal research included 24 class III obesity subjects with a BMI > 40 kg/m<sup>2</sup>. The subjects were enrolled from the Metabolic/Surgical Department at Hamad Medical Corporation (HMC)-Qatar. Fasting blood samples were collected at admission to LGS for weight loss and after 12 weeks of LGS. The immunophenotype of CD4+ T-cell populations; naïve (CD45RA<sup>+</sup>and CD27<sup>+</sup>), central memory T cells (CD45RO<sup>+</sup> and CD27<sup>+</sup>), and effector memory (CD45RO<sup>+</sup>and CD27<sup>−</sup>) and T-regulatory cell (CD4<sup>+</sup>CD25<sup>+</sup> FoxP3<sup>+</sup>) were identified using flow cytometry. Plasma pro-inflammatory cytokines and adipokines were evaluated. A control group of lean subjects was used to compare changes of T-regulatory and inflammatory biomarkers with postoperative changes in obese patients. </p><h3>Results </h3><p dir="ltr">The means (SD) of age and BMI of class III obesity subjects was 32.32 (8.36) years and 49.02 (6.28) kg/m<sup>2</sup>, respectively. LGS caused a significant reduction in BMI by 32%, p< 0.0001. LGS intervention significantly decreased CD4+ T-lymphocytes and effector memory (TEM) cells but increased T-regulatory (Treg), naïve, and central memory (TCM) cells, with all p values < 0.05. The increase of Treg cells postoperative is significantly lower compared to lean subjects, p < 0.05. A significant reduction of plasma IL-6, TNF-α, and MCP-1, but IL-10 significantly increased after LGS, with all p< 0.05. Adiponectin/leptin ratio improved after LGS by 2.9 folds, p< 0.0001. </p><h3>Conclusion </h3><p dir="ltr">Weight loss by LGS accomplished a substantial rise of Treg and decreased EM T-lymphocytes with a shift from pro-inflammatory to the anti-inflammatory pattern.</p><h2>Other Information</h2><p dir="ltr">Published in: Journal of Inflammation Research<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.2147/jir.s282189" target="_blank">https://dx.doi.org/10.2147/jir.s282189</a></p>2021-05-01T00:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.2147/jir.s282189https://figshare.com/articles/journal_contribution/The_Immunophenotyping_Changes_of_Peripheral_CD4_T_Lymphocytes_and_Inflammatory_Markers_of_Class_III_Obesity_Subjects_After_Laparoscopic_Gastric_Sleeve_Surgery_A_Follow-Up_Study/26020930CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/260209302021-05-01T00:00:00Z |
| spellingShingle | The Immunophenotyping Changes of Peripheral CD4+ T Lymphocytes and Inflammatory Markers of Class III Obesity Subjects After Laparoscopic Gastric Sleeve Surgery – A Follow-Up Study Nasser M Rizk (18810328) Biomedical and clinical sciences Clinical sciences Immunology class III obesity immunity peripheral CD4+ T-lymphocytes T-regulatory cells follow up laparoscope gastric sleeve surgery |
| status_str | publishedVersion |
| title | The Immunophenotyping Changes of Peripheral CD4+ T Lymphocytes and Inflammatory Markers of Class III Obesity Subjects After Laparoscopic Gastric Sleeve Surgery – A Follow-Up Study |
| title_full | The Immunophenotyping Changes of Peripheral CD4+ T Lymphocytes and Inflammatory Markers of Class III Obesity Subjects After Laparoscopic Gastric Sleeve Surgery – A Follow-Up Study |
| title_fullStr | The Immunophenotyping Changes of Peripheral CD4+ T Lymphocytes and Inflammatory Markers of Class III Obesity Subjects After Laparoscopic Gastric Sleeve Surgery – A Follow-Up Study |
| title_full_unstemmed | The Immunophenotyping Changes of Peripheral CD4+ T Lymphocytes and Inflammatory Markers of Class III Obesity Subjects After Laparoscopic Gastric Sleeve Surgery – A Follow-Up Study |
| title_short | The Immunophenotyping Changes of Peripheral CD4+ T Lymphocytes and Inflammatory Markers of Class III Obesity Subjects After Laparoscopic Gastric Sleeve Surgery – A Follow-Up Study |
| title_sort | The Immunophenotyping Changes of Peripheral CD4+ T Lymphocytes and Inflammatory Markers of Class III Obesity Subjects After Laparoscopic Gastric Sleeve Surgery – A Follow-Up Study |
| topic | Biomedical and clinical sciences Clinical sciences Immunology class III obesity immunity peripheral CD4+ T-lymphocytes T-regulatory cells follow up laparoscope gastric sleeve surgery |