Divergent wiring of repressive and active chromatin interactions between mouse embryonic and trophoblast lineages
<div><p>The establishment of the embryonic and trophoblast lineages is a developmental decision underpinned by dramatic differences in the epigenetic landscape of the two compartments. However, it remains unknown how epigenetic information and transcription factor networks map to the 3D...
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2018
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| _version_ | 1864513513530064896 |
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| author | Stefan Schoenfelder (115930) |
| author2 | Borbala Mifsud (3907267) Claire E. Senner (16450827) Christopher D. Todd (396550) Stephanie Chrysanthou (18629476) Elodie Darbo (3907261) Myriam Hemberger (93502) Miguel R. Branco (16450833) |
| author2_role | author author author author author author author |
| author_facet | Stefan Schoenfelder (115930) Borbala Mifsud (3907267) Claire E. Senner (16450827) Christopher D. Todd (396550) Stephanie Chrysanthou (18629476) Elodie Darbo (3907261) Myriam Hemberger (93502) Miguel R. Branco (16450833) |
| author_role | author |
| dc.creator.none.fl_str_mv | Stefan Schoenfelder (115930) Borbala Mifsud (3907267) Claire E. Senner (16450827) Christopher D. Todd (396550) Stephanie Chrysanthou (18629476) Elodie Darbo (3907261) Myriam Hemberger (93502) Miguel R. Branco (16450833) |
| dc.date.none.fl_str_mv | 2018-10-10T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1038/s41467-018-06666-4 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Divergent_wiring_of_repressive_and_active_chromatin_interactions_between_mouse_embryonic_and_trophoblast_lineages/25920661 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Genetics Embryonic development Trophoblast lineage Epigenetic landscape Chromatin conformation 3D genome arrangement |
| dc.title.none.fl_str_mv | Divergent wiring of repressive and active chromatin interactions between mouse embryonic and trophoblast lineages |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <div><p>The establishment of the embryonic and trophoblast lineages is a developmental decision underpinned by dramatic differences in the epigenetic landscape of the two compartments. However, it remains unknown how epigenetic information and transcription factor networks map to the 3D arrangement of the genome, which in turn may mediate transcriptional divergence between the two cell lineages. Here, we perform promoter capture Hi-C experiments in mouse trophoblast (TSC) and embryonic (ESC) stem cells to understand how chromatin conformation relates to cell-specific transcriptional programmes. We find that key TSC genes that are kept repressed in ESCs exhibit interactions between H3K27me3-marked regions in ESCs that depend on Polycomb repressive complex 1. Interactions that are prominent in TSCs are enriched for enhancer–gene contacts involving key TSC transcription factors, as well as TET1, which helps to maintain the expression of TSC-relevant genes. Our work shows that the first developmental cell fate decision results in distinct chromatin conformation patterns establishing lineage-specific contexts involving both repressive and active interactions.</p><p> </p></div><h2>Other Information</h2> <p> Published in: Nature Communications<br> License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1038/s41467-018-06666-4" target="_blank">https://dx.doi.org/10.1038/s41467-018-06666-4</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_cf39d8d476b45a49cbdf264b59e27594 |
| identifier_str_mv | 10.1038/s41467-018-06666-4 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25920661 |
| publishDate | 2018 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Divergent wiring of repressive and active chromatin interactions between mouse embryonic and trophoblast lineagesStefan Schoenfelder (115930)Borbala Mifsud (3907267)Claire E. Senner (16450827)Christopher D. Todd (396550)Stephanie Chrysanthou (18629476)Elodie Darbo (3907261)Myriam Hemberger (93502)Miguel R. Branco (16450833)Biological sciencesGeneticsEmbryonic developmentTrophoblast lineageEpigenetic landscapeChromatin conformation3D genome arrangement<div><p>The establishment of the embryonic and trophoblast lineages is a developmental decision underpinned by dramatic differences in the epigenetic landscape of the two compartments. However, it remains unknown how epigenetic information and transcription factor networks map to the 3D arrangement of the genome, which in turn may mediate transcriptional divergence between the two cell lineages. Here, we perform promoter capture Hi-C experiments in mouse trophoblast (TSC) and embryonic (ESC) stem cells to understand how chromatin conformation relates to cell-specific transcriptional programmes. We find that key TSC genes that are kept repressed in ESCs exhibit interactions between H3K27me3-marked regions in ESCs that depend on Polycomb repressive complex 1. Interactions that are prominent in TSCs are enriched for enhancer–gene contacts involving key TSC transcription factors, as well as TET1, which helps to maintain the expression of TSC-relevant genes. Our work shows that the first developmental cell fate decision results in distinct chromatin conformation patterns establishing lineage-specific contexts involving both repressive and active interactions.</p><p> </p></div><h2>Other Information</h2> <p> Published in: Nature Communications<br> License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1038/s41467-018-06666-4" target="_blank">https://dx.doi.org/10.1038/s41467-018-06666-4</a></p>2018-10-10T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1038/s41467-018-06666-4https://figshare.com/articles/journal_contribution/Divergent_wiring_of_repressive_and_active_chromatin_interactions_between_mouse_embryonic_and_trophoblast_lineages/25920661CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/259206612018-10-10T03:00:00Z |
| spellingShingle | Divergent wiring of repressive and active chromatin interactions between mouse embryonic and trophoblast lineages Stefan Schoenfelder (115930) Biological sciences Genetics Embryonic development Trophoblast lineage Epigenetic landscape Chromatin conformation 3D genome arrangement |
| status_str | publishedVersion |
| title | Divergent wiring of repressive and active chromatin interactions between mouse embryonic and trophoblast lineages |
| title_full | Divergent wiring of repressive and active chromatin interactions between mouse embryonic and trophoblast lineages |
| title_fullStr | Divergent wiring of repressive and active chromatin interactions between mouse embryonic and trophoblast lineages |
| title_full_unstemmed | Divergent wiring of repressive and active chromatin interactions between mouse embryonic and trophoblast lineages |
| title_short | Divergent wiring of repressive and active chromatin interactions between mouse embryonic and trophoblast lineages |
| title_sort | Divergent wiring of repressive and active chromatin interactions between mouse embryonic and trophoblast lineages |
| topic | Biological sciences Genetics Embryonic development Trophoblast lineage Epigenetic landscape Chromatin conformation 3D genome arrangement |