Physicochemical characterization and cancer cell antiproliferative effect of silver-doped magnesia nanoparticles
<p>Silver-doped magnesia nanoparticles (Ag/MgO) were synthesized using the precipitation method and characterized by various techniques such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), thermal gravimetric analysis (TGA), Brunner-Emmett-Teller (BET) surface area...
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2023
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| _version_ | 1864513529365659648 |
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| author | Mohamed Qasim Al-Fahdawi (17844209) |
| author2 | Ahmed Faris Aldoghachi (16301714) Fatah H. Alhassan (17844212) Faris A.J. Al-Doghachi (17844215) Hussah Abdullah Alshwyeh (16621140) Abdullah Rasedee (6940799) Sulaiman Mohammed Alnasser (16713520) Mothanna Sadiq Al-Qubaisi (17844218) Wisam Nabeel Ibrahim (13899337) |
| author2_role | author author author author author author author author |
| author_facet | Mohamed Qasim Al-Fahdawi (17844209) Ahmed Faris Aldoghachi (16301714) Fatah H. Alhassan (17844212) Faris A.J. Al-Doghachi (17844215) Hussah Abdullah Alshwyeh (16621140) Abdullah Rasedee (6940799) Sulaiman Mohammed Alnasser (16713520) Mothanna Sadiq Al-Qubaisi (17844218) Wisam Nabeel Ibrahim (13899337) |
| author_role | author |
| dc.creator.none.fl_str_mv | Mohamed Qasim Al-Fahdawi (17844209) Ahmed Faris Aldoghachi (16301714) Fatah H. Alhassan (17844212) Faris A.J. Al-Doghachi (17844215) Hussah Abdullah Alshwyeh (16621140) Abdullah Rasedee (6940799) Sulaiman Mohammed Alnasser (16713520) Mothanna Sadiq Al-Qubaisi (17844218) Wisam Nabeel Ibrahim (13899337) |
| dc.date.none.fl_str_mv | 2023-04-17T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1016/j.heliyon.2023.e15560 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Physicochemical_characterization_and_cancer_cell_antiproliferative_effect_of_silver-doped_magnesia_nanoparticles/25059731 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Clinical sciences Pharmacology and pharmaceutical sciences Ag/MgO nanoparticles HT29 cell A549 cell Antiproliferative Apoptosis |
| dc.title.none.fl_str_mv | Physicochemical characterization and cancer cell antiproliferative effect of silver-doped magnesia nanoparticles |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p>Silver-doped magnesia nanoparticles (Ag/MgO) were synthesized using the precipitation method and characterized by various techniques such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), thermal gravimetric analysis (TGA), Brunner-Emmett-Teller (BET) surface area measurements, and dispersive X-ray spectroscopy (EDX). The morphology of Ag/MgO nanoparticles was determined by transmission and scanning electron microscopy, which revealed cuboidal shaped nanoparticles with sizes ranging from 31 to 68 nm and an average size of 43.5 ± 10.6 nm. The anticancer effects of Ag/MgO nanoparticles were evaluated on human colorectal (HT29) and lung adenocarcinoma (A549) cell lines, and their caspase-3, -8, and -9 activities, as well as Bcl-2, Bax, p53, cytochrome C protein expressions were estimated. Ag/MgO nanoparticles showed selective toxicity towards HT29 and A549 cells while remaining relatively innocuous towards the normal human colorectal, CCD-18Co, and lung, MRC-5 cells. The IC50 values of Ag/MgO nanoparticles on the HT29 and A549 cells were found to be 90.2 ± 2.6 and 85.0 ± 3.5 μg/mL, respectively. The Ag/MgO nanoparticles upregulated caspase-3 and -9 activities, downregulated Bcl-2, upregulated Bax and p53 protein expressions in the cancer cells. The morphology of the Ag/MgO nanoparticle treated HT29 and A549 cells was typical of apoptosis, with cell detachment, shrinkage, and membrane blebbing. The results suggest that Ag/MgO nanoparticles induce apoptosis in cancer cells and exhibit potential as a promising anticancer agent.</p><h2>Other Information</h2> <p> Published in: Heliyon<br> License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.heliyon.2023.e15560" target="_blank">https://dx.doi.org/10.1016/j.heliyon.2023.e15560</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_cf65bf456e3c16fc986f8144f2a2bfde |
| identifier_str_mv | 10.1016/j.heliyon.2023.e15560 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25059731 |
| publishDate | 2023 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Physicochemical characterization and cancer cell antiproliferative effect of silver-doped magnesia nanoparticlesMohamed Qasim Al-Fahdawi (17844209)Ahmed Faris Aldoghachi (16301714)Fatah H. Alhassan (17844212)Faris A.J. Al-Doghachi (17844215)Hussah Abdullah Alshwyeh (16621140)Abdullah Rasedee (6940799)Sulaiman Mohammed Alnasser (16713520)Mothanna Sadiq Al-Qubaisi (17844218)Wisam Nabeel Ibrahim (13899337)Biomedical and clinical sciencesClinical sciencesPharmacology and pharmaceutical sciencesAg/MgO nanoparticlesHT29 cellA549 cellAntiproliferativeApoptosis<p>Silver-doped magnesia nanoparticles (Ag/MgO) were synthesized using the precipitation method and characterized by various techniques such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), thermal gravimetric analysis (TGA), Brunner-Emmett-Teller (BET) surface area measurements, and dispersive X-ray spectroscopy (EDX). The morphology of Ag/MgO nanoparticles was determined by transmission and scanning electron microscopy, which revealed cuboidal shaped nanoparticles with sizes ranging from 31 to 68 nm and an average size of 43.5 ± 10.6 nm. The anticancer effects of Ag/MgO nanoparticles were evaluated on human colorectal (HT29) and lung adenocarcinoma (A549) cell lines, and their caspase-3, -8, and -9 activities, as well as Bcl-2, Bax, p53, cytochrome C protein expressions were estimated. Ag/MgO nanoparticles showed selective toxicity towards HT29 and A549 cells while remaining relatively innocuous towards the normal human colorectal, CCD-18Co, and lung, MRC-5 cells. The IC50 values of Ag/MgO nanoparticles on the HT29 and A549 cells were found to be 90.2 ± 2.6 and 85.0 ± 3.5 μg/mL, respectively. The Ag/MgO nanoparticles upregulated caspase-3 and -9 activities, downregulated Bcl-2, upregulated Bax and p53 protein expressions in the cancer cells. The morphology of the Ag/MgO nanoparticle treated HT29 and A549 cells was typical of apoptosis, with cell detachment, shrinkage, and membrane blebbing. The results suggest that Ag/MgO nanoparticles induce apoptosis in cancer cells and exhibit potential as a promising anticancer agent.</p><h2>Other Information</h2> <p> Published in: Heliyon<br> License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.heliyon.2023.e15560" target="_blank">https://dx.doi.org/10.1016/j.heliyon.2023.e15560</a></p>2023-04-17T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1016/j.heliyon.2023.e15560https://figshare.com/articles/journal_contribution/Physicochemical_characterization_and_cancer_cell_antiproliferative_effect_of_silver-doped_magnesia_nanoparticles/25059731CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/250597312023-04-17T03:00:00Z |
| spellingShingle | Physicochemical characterization and cancer cell antiproliferative effect of silver-doped magnesia nanoparticles Mohamed Qasim Al-Fahdawi (17844209) Biomedical and clinical sciences Clinical sciences Pharmacology and pharmaceutical sciences Ag/MgO nanoparticles HT29 cell A549 cell Antiproliferative Apoptosis |
| status_str | publishedVersion |
| title | Physicochemical characterization and cancer cell antiproliferative effect of silver-doped magnesia nanoparticles |
| title_full | Physicochemical characterization and cancer cell antiproliferative effect of silver-doped magnesia nanoparticles |
| title_fullStr | Physicochemical characterization and cancer cell antiproliferative effect of silver-doped magnesia nanoparticles |
| title_full_unstemmed | Physicochemical characterization and cancer cell antiproliferative effect of silver-doped magnesia nanoparticles |
| title_short | Physicochemical characterization and cancer cell antiproliferative effect of silver-doped magnesia nanoparticles |
| title_sort | Physicochemical characterization and cancer cell antiproliferative effect of silver-doped magnesia nanoparticles |
| topic | Biomedical and clinical sciences Clinical sciences Pharmacology and pharmaceutical sciences Ag/MgO nanoparticles HT29 cell A549 cell Antiproliferative Apoptosis |