Crumpled MXene nanosheets for sensing of ascorbic acid in food, biological fluids, and erythrocytes in-vitro microenvironment
<p dir="ltr">In this work, a simple and facile method was developed to achieve controlled oxidation and enhance the surface area of MXene nanosheets and their utilization in the efficient sensing of ascorbic acid (AA or vitamin C). After etching of MAX phase to MXene via the MILD tec...
محفوظ في:
| المؤلف الرئيسي: | |
|---|---|
| مؤلفون آخرون: | , , |
| منشور في: |
2023
|
| الموضوعات: | |
| الوسوم: |
إضافة وسم
لا توجد وسوم, كن أول من يضع وسما على هذه التسجيلة!
|
| _version_ | 1864513557783117824 |
|---|---|
| author | Shabi Abbas Zaidi (4234354) |
| author2 | Hanan Sheikh (16810743) Muna Al-Mahasna (16810746) Fathiya Elsin (16810749) |
| author2_role | author author author |
| author_facet | Shabi Abbas Zaidi (4234354) Hanan Sheikh (16810743) Muna Al-Mahasna (16810746) Fathiya Elsin (16810749) |
| author_role | author |
| dc.creator.none.fl_str_mv | Shabi Abbas Zaidi (4234354) Hanan Sheikh (16810743) Muna Al-Mahasna (16810746) Fathiya Elsin (16810749) |
| dc.date.none.fl_str_mv | 2023-09-30T00:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1016/j.ijbiomac.2023.126024 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Crumpled_MXene_nanosheets_for_sensing_of_ascorbic_acid_in_food_biological_fluids_and_erythrocytes_in-vitro_microenvironment/24173181 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Biochemistry and cell biology Chemical sciences Medicinal and biomolecular chemistry MXene Nanosheets Partial oxidation Crumple Surface area Electrochemical sensor Ascorbic acid In vitro |
| dc.title.none.fl_str_mv | Crumpled MXene nanosheets for sensing of ascorbic acid in food, biological fluids, and erythrocytes in-vitro microenvironment |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">In this work, a simple and facile method was developed to achieve controlled oxidation and enhance the surface area of MXene nanosheets and their utilization in the efficient sensing of ascorbic acid (AA or vitamin C). After etching of MAX phase to MXene via the MILD technique, controlled flash oxidation was carried out in the open air environment for 1.5 h, followed by flocculation of oxidized MXene nanosheets by using H<sub>2</sub>SO<sub>4</sub>, consequently achieving crumpled MXene possessing anatase phase, porosity, and improved surface area as revealed and confirmed by SEM, TEM, Raman, and BET analysis results. The as-prepared crumpled MXene was coated over a glassy carbon electrode (GCE) and used to determine AA successfully via cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV) with a linear concentration range of 300 μM to 0.005 μM with a detection limit (LOD) of 2 nM (2.8 % RSD and S/N = 3). The developed electrochemical sensor was used to determine the AA in various actual samples such as juice, urine, serum, and erythrocytes spiked with AA with excellent recoveries in the 94–103 % range. The sensor also demonstrated excellent reproducibility (~1 % RSD for five repetitive assays) and a shelf life of nearly one month with a negligible decrease in response. Furthermore, it lost only 10 % of its response for the next ten days. It also showed satisfactory selectivity toward AA in the presence of other similar compounds, including uric acid (UA), dopamine (DA), and glucose.</p><h2>Other Information</h2><p dir="ltr">Published in: International Journal of Biological Macromolecules<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.ijbiomac.2023.126024" target="_blank">https://dx.doi.org/10.1016/j.ijbiomac.2023.126024</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_cfc44ecfb800eba2322b6d7a11d0f951 |
| identifier_str_mv | 10.1016/j.ijbiomac.2023.126024 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/24173181 |
| publishDate | 2023 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Crumpled MXene nanosheets for sensing of ascorbic acid in food, biological fluids, and erythrocytes in-vitro microenvironmentShabi Abbas Zaidi (4234354)Hanan Sheikh (16810743)Muna Al-Mahasna (16810746)Fathiya Elsin (16810749)Biological sciencesBiochemistry and cell biologyChemical sciencesMedicinal and biomolecular chemistryMXeneNanosheetsPartial oxidationCrumpleSurface areaElectrochemical sensorAscorbic acidIn vitro<p dir="ltr">In this work, a simple and facile method was developed to achieve controlled oxidation and enhance the surface area of MXene nanosheets and their utilization in the efficient sensing of ascorbic acid (AA or vitamin C). After etching of MAX phase to MXene via the MILD technique, controlled flash oxidation was carried out in the open air environment for 1.5 h, followed by flocculation of oxidized MXene nanosheets by using H<sub>2</sub>SO<sub>4</sub>, consequently achieving crumpled MXene possessing anatase phase, porosity, and improved surface area as revealed and confirmed by SEM, TEM, Raman, and BET analysis results. The as-prepared crumpled MXene was coated over a glassy carbon electrode (GCE) and used to determine AA successfully via cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV) with a linear concentration range of 300 μM to 0.005 μM with a detection limit (LOD) of 2 nM (2.8 % RSD and S/N = 3). The developed electrochemical sensor was used to determine the AA in various actual samples such as juice, urine, serum, and erythrocytes spiked with AA with excellent recoveries in the 94–103 % range. The sensor also demonstrated excellent reproducibility (~1 % RSD for five repetitive assays) and a shelf life of nearly one month with a negligible decrease in response. Furthermore, it lost only 10 % of its response for the next ten days. It also showed satisfactory selectivity toward AA in the presence of other similar compounds, including uric acid (UA), dopamine (DA), and glucose.</p><h2>Other Information</h2><p dir="ltr">Published in: International Journal of Biological Macromolecules<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.ijbiomac.2023.126024" target="_blank">https://dx.doi.org/10.1016/j.ijbiomac.2023.126024</a></p>2023-09-30T00:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1016/j.ijbiomac.2023.126024https://figshare.com/articles/journal_contribution/Crumpled_MXene_nanosheets_for_sensing_of_ascorbic_acid_in_food_biological_fluids_and_erythrocytes_in-vitro_microenvironment/24173181CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/241731812023-09-30T00:00:00Z |
| spellingShingle | Crumpled MXene nanosheets for sensing of ascorbic acid in food, biological fluids, and erythrocytes in-vitro microenvironment Shabi Abbas Zaidi (4234354) Biological sciences Biochemistry and cell biology Chemical sciences Medicinal and biomolecular chemistry MXene Nanosheets Partial oxidation Crumple Surface area Electrochemical sensor Ascorbic acid In vitro |
| status_str | publishedVersion |
| title | Crumpled MXene nanosheets for sensing of ascorbic acid in food, biological fluids, and erythrocytes in-vitro microenvironment |
| title_full | Crumpled MXene nanosheets for sensing of ascorbic acid in food, biological fluids, and erythrocytes in-vitro microenvironment |
| title_fullStr | Crumpled MXene nanosheets for sensing of ascorbic acid in food, biological fluids, and erythrocytes in-vitro microenvironment |
| title_full_unstemmed | Crumpled MXene nanosheets for sensing of ascorbic acid in food, biological fluids, and erythrocytes in-vitro microenvironment |
| title_short | Crumpled MXene nanosheets for sensing of ascorbic acid in food, biological fluids, and erythrocytes in-vitro microenvironment |
| title_sort | Crumpled MXene nanosheets for sensing of ascorbic acid in food, biological fluids, and erythrocytes in-vitro microenvironment |
| topic | Biological sciences Biochemistry and cell biology Chemical sciences Medicinal and biomolecular chemistry MXene Nanosheets Partial oxidation Crumple Surface area Electrochemical sensor Ascorbic acid In vitro |