Size-resolved ambient bioaerosols concentration, antibiotic resistance, and community composition during autumn and winter seasons in Qatar
<p dir="ltr">This study investigates the size distribution, microbial composition, and antibiotic resistance (ABR) of airborne bioaerosols at a suburban location in Doha, Qatar between October 2021 and January 2022. Samples were collected using an Andersen six-stage viable cascade im...
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2023
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| _version_ | 1864513549542359040 |
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| author | Bilal Sajjad (17017749) |
| author2 | Kashif Rasool (2542492) Azhar Siddique (1770661) Khadeeja Abdul Jabbar (17017752) Shimaa S. El-Malaha (17017755) Muhammad Umar Sohail (11995001) Fares Almomani (12585685) M. Rami Alfarra (1343493) |
| author2_role | author author author author author author author |
| author_facet | Bilal Sajjad (17017749) Kashif Rasool (2542492) Azhar Siddique (1770661) Khadeeja Abdul Jabbar (17017752) Shimaa S. El-Malaha (17017755) Muhammad Umar Sohail (11995001) Fares Almomani (12585685) M. Rami Alfarra (1343493) |
| author_role | author |
| dc.creator.none.fl_str_mv | Bilal Sajjad (17017749) Kashif Rasool (2542492) Azhar Siddique (1770661) Khadeeja Abdul Jabbar (17017752) Shimaa S. El-Malaha (17017755) Muhammad Umar Sohail (11995001) Fares Almomani (12585685) M. Rami Alfarra (1343493) |
| dc.date.none.fl_str_mv | 2023-11-01T00:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1016/j.envpol.2023.122401 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Size-resolved_ambient_bioaerosols_concentration_antibiotic_resistance_and_community_composition_during_autumn_and_winter_seasons_in_Qatar/24173169 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Pharmacology and pharmaceutical sciences Earth sciences Atmospheric sciences Engineering Environmental engineering Bioaerosols Particulate matter Particle size distribution DNA sequencing Antibiotic |
| dc.title.none.fl_str_mv | Size-resolved ambient bioaerosols concentration, antibiotic resistance, and community composition during autumn and winter seasons in Qatar |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">This study investigates the size distribution, microbial composition, and antibiotic resistance (ABR) of airborne bioaerosols at a suburban location in Doha, Qatar between October 2021 and January 2022. Samples were collected using an Andersen six-stage viable cascade impactor and a liquid impinger. Findings showed that the mean bacteria concentration (464 CFU/m<sup>3</sup>) was significantly higher than that of fungi (242 CFU/m<sup>3</sup>) during the study period. Both bacteria and fungi were most abundant in the aerodynamic size fractions of 1.10–2.21 μm, with peak concentrations observed in the mornings and lowest concentrations in the afternoons across all size fractions. A total of 24 different culturable species were identified, with the most abundant ones being <i>Pasteurella pneumotropica</i> (9.71%), <i>Pantoea</i> spp. 1 (8.73%), and <i>Proteus penneri</i> (7.77%) <i>spp</i>. At the phylum level, the bacterial community configurations during the autumn and winter seasons were nearly identical as revealed by molecular genomics, with <i>Proteobacteria</i> being the most predominant, followed by <i>Firmicutes, Bacteroidetes, Acidobacteriota</i>, and <i>Planctomycetota</i>. However, there was a significant variation in dominant genera between autumn and winter. The most abundant genera included <i>Sphingomonas, Paraburkholderia, Comamonas, Bacillus</i>, and <i>Lysinibacillus</i>. Several bacterial genera identified in this study have important public health and ecological implications, including the risk of respiratory tract infections. Furthermore, the study found that ABR was highest in December, with bioaerosols exhibiting resistance to at least 5 out of 10 antibiotics, and 100% resistance to Metronidazole in all samples. Metagenomics analysis revealed the presence of various airborne bacteria that were not detected through culture-dependent methods. This study provides valuable insights into the airborne microbial composition, temporal variability and ABR in the Arabian Gulf region..</p><h2>Other Information</h2><p dir="ltr">Published in: Environmental Pollution<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.envpol.2023.122401" target="_blank">https://dx.doi.org/10.1016/j.envpol.2023.122401</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_d00c6f7c7ad61d0ab0a592e9be1a572c |
| identifier_str_mv | 10.1016/j.envpol.2023.122401 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/24173169 |
| publishDate | 2023 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Size-resolved ambient bioaerosols concentration, antibiotic resistance, and community composition during autumn and winter seasons in QatarBilal Sajjad (17017749)Kashif Rasool (2542492)Azhar Siddique (1770661)Khadeeja Abdul Jabbar (17017752)Shimaa S. El-Malaha (17017755)Muhammad Umar Sohail (11995001)Fares Almomani (12585685)M. Rami Alfarra (1343493)Biomedical and clinical sciencesPharmacology and pharmaceutical sciencesEarth sciencesAtmospheric sciencesEngineeringEnvironmental engineeringBioaerosolsParticulate matterParticle size distributionDNA sequencingAntibiotic<p dir="ltr">This study investigates the size distribution, microbial composition, and antibiotic resistance (ABR) of airborne bioaerosols at a suburban location in Doha, Qatar between October 2021 and January 2022. Samples were collected using an Andersen six-stage viable cascade impactor and a liquid impinger. Findings showed that the mean bacteria concentration (464 CFU/m<sup>3</sup>) was significantly higher than that of fungi (242 CFU/m<sup>3</sup>) during the study period. Both bacteria and fungi were most abundant in the aerodynamic size fractions of 1.10–2.21 μm, with peak concentrations observed in the mornings and lowest concentrations in the afternoons across all size fractions. A total of 24 different culturable species were identified, with the most abundant ones being <i>Pasteurella pneumotropica</i> (9.71%), <i>Pantoea</i> spp. 1 (8.73%), and <i>Proteus penneri</i> (7.77%) <i>spp</i>. At the phylum level, the bacterial community configurations during the autumn and winter seasons were nearly identical as revealed by molecular genomics, with <i>Proteobacteria</i> being the most predominant, followed by <i>Firmicutes, Bacteroidetes, Acidobacteriota</i>, and <i>Planctomycetota</i>. However, there was a significant variation in dominant genera between autumn and winter. The most abundant genera included <i>Sphingomonas, Paraburkholderia, Comamonas, Bacillus</i>, and <i>Lysinibacillus</i>. Several bacterial genera identified in this study have important public health and ecological implications, including the risk of respiratory tract infections. Furthermore, the study found that ABR was highest in December, with bioaerosols exhibiting resistance to at least 5 out of 10 antibiotics, and 100% resistance to Metronidazole in all samples. Metagenomics analysis revealed the presence of various airborne bacteria that were not detected through culture-dependent methods. This study provides valuable insights into the airborne microbial composition, temporal variability and ABR in the Arabian Gulf region..</p><h2>Other Information</h2><p dir="ltr">Published in: Environmental Pollution<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.envpol.2023.122401" target="_blank">https://dx.doi.org/10.1016/j.envpol.2023.122401</a></p>2023-11-01T00:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1016/j.envpol.2023.122401https://figshare.com/articles/journal_contribution/Size-resolved_ambient_bioaerosols_concentration_antibiotic_resistance_and_community_composition_during_autumn_and_winter_seasons_in_Qatar/24173169CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/241731692023-11-01T00:00:00Z |
| spellingShingle | Size-resolved ambient bioaerosols concentration, antibiotic resistance, and community composition during autumn and winter seasons in Qatar Bilal Sajjad (17017749) Biomedical and clinical sciences Pharmacology and pharmaceutical sciences Earth sciences Atmospheric sciences Engineering Environmental engineering Bioaerosols Particulate matter Particle size distribution DNA sequencing Antibiotic |
| status_str | publishedVersion |
| title | Size-resolved ambient bioaerosols concentration, antibiotic resistance, and community composition during autumn and winter seasons in Qatar |
| title_full | Size-resolved ambient bioaerosols concentration, antibiotic resistance, and community composition during autumn and winter seasons in Qatar |
| title_fullStr | Size-resolved ambient bioaerosols concentration, antibiotic resistance, and community composition during autumn and winter seasons in Qatar |
| title_full_unstemmed | Size-resolved ambient bioaerosols concentration, antibiotic resistance, and community composition during autumn and winter seasons in Qatar |
| title_short | Size-resolved ambient bioaerosols concentration, antibiotic resistance, and community composition during autumn and winter seasons in Qatar |
| title_sort | Size-resolved ambient bioaerosols concentration, antibiotic resistance, and community composition during autumn and winter seasons in Qatar |
| topic | Biomedical and clinical sciences Pharmacology and pharmaceutical sciences Earth sciences Atmospheric sciences Engineering Environmental engineering Bioaerosols Particulate matter Particle size distribution DNA sequencing Antibiotic |