N-Lactoyl amino acids: insights from metabolite genome-wide association studies and phenome-wide association analysis

<p dir="ltr">N-lactoyl-amino acids (Lac-AA) are emerging as important metabolites with diverse physiological roles. This study integrates metabolomics and genomics to investigate the genetic determinants and clinical relevance of three Lac-AA: N-Lactoyl phenylalanine (Lac-Phe), N-Lac...

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Main Author: Asma A Elashi (18464133) (author)
Other Authors: Aleem Razzaq (14779189) (author), Najeha Anwardeen (11924495) (author), Khaled Naja (17886740) (author), Mashael Alshafai (14778607) (author), Ilhame Diboun (3522413) (author), Omar Albagha (8977856) (author), Mohamed A Elrayess (11843435) (author)
Published: 2025
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author Asma A Elashi (18464133)
author2 Aleem Razzaq (14779189)
Najeha Anwardeen (11924495)
Khaled Naja (17886740)
Mashael Alshafai (14778607)
Ilhame Diboun (3522413)
Omar Albagha (8977856)
Mohamed A Elrayess (11843435)
author2_role author
author
author
author
author
author
author
author_facet Asma A Elashi (18464133)
Aleem Razzaq (14779189)
Najeha Anwardeen (11924495)
Khaled Naja (17886740)
Mashael Alshafai (14778607)
Ilhame Diboun (3522413)
Omar Albagha (8977856)
Mohamed A Elrayess (11843435)
author_role author
dc.creator.none.fl_str_mv Asma A Elashi (18464133)
Aleem Razzaq (14779189)
Najeha Anwardeen (11924495)
Khaled Naja (17886740)
Mashael Alshafai (14778607)
Ilhame Diboun (3522413)
Omar Albagha (8977856)
Mohamed A Elrayess (11843435)
dc.date.none.fl_str_mv 2025-09-28T09:00:00Z
dc.identifier.none.fl_str_mv 10.1093/hmg/ddaf152
dc.relation.none.fl_str_mv https://figshare.com/articles/journal_contribution/N-Lactoyl_amino_acids_insights_from_metabolite_genome-wide_association_studies_and_phenome-wide_association_analysis/31168186
dc.rights.none.fl_str_mv CC BY 4.0
info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Biological sciences
Genetics
Biomedical and clinical sciences
Immunology
Medical biochemistry and metabolomics
N-Lactoyl amino acids
mGWAS
PheWAS
Metabolomics
dc.title.none.fl_str_mv N-Lactoyl amino acids: insights from metabolite genome-wide association studies and phenome-wide association analysis
dc.type.none.fl_str_mv Text
Journal contribution
info:eu-repo/semantics/publishedVersion
text
contribution to journal
description <p dir="ltr">N-lactoyl-amino acids (Lac-AA) are emerging as important metabolites with diverse physiological roles. This study integrates metabolomics and genomics to investigate the genetic determinants and clinical relevance of three Lac-AA: N-Lactoyl phenylalanine (Lac-Phe), N-Lactoyl tyrosine (Lac-Tyr), and N-Lactoyl valine (Lac-Tyr). We conducted a metabolome-wide association study (mGWAS) on 2811 participants followed by a phenome-wide association study (PheWAS) and pathway enrichment analysis. Our mGWAS revealed modest genetic contributions to Lac-AA levels, with genome-wide significant loci identified for Lac-Tyr and Lac-Val, but not for Lac-Phe. PheWAS analysis linked these genetic variants to key clinical traits, including white blood cell count, platelet count, and glucose levels. Pathway enrichment highlighted the involvement of Lac-AA in immune-metabolic crosstalk, particularly in inflammation and energy metabolism. These findings suggest that Lac-AA levels are primarily influenced by dynamic metabolic or inflammatory states rather than fixed genetic factors. Our results underscore the potential of Lac-AA as metabolic sensors and biomarkers at the intersection of cellular energy states and systemic inflammation, opening new avenues for research in metabolic and inflammatory disorders.</p><h2 dir="ltr">Other Information</h2><p dir="ltr">Published in: Human Molecular Genetics<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1093/hmg/ddaf152" target="_blank">https://dx.doi.org/10.1093/hmg/ddaf152</a></p>
eu_rights_str_mv openAccess
id Manara2_d08ee2329171286d75a6382a89ee6b88
identifier_str_mv 10.1093/hmg/ddaf152
network_acronym_str Manara2
network_name_str Manara2
oai_identifier_str oai:figshare.com:article/31168186
publishDate 2025
repository.mail.fl_str_mv
repository.name.fl_str_mv
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rights_invalid_str_mv CC BY 4.0
spelling N-Lactoyl amino acids: insights from metabolite genome-wide association studies and phenome-wide association analysisAsma A Elashi (18464133)Aleem Razzaq (14779189)Najeha Anwardeen (11924495)Khaled Naja (17886740)Mashael Alshafai (14778607)Ilhame Diboun (3522413)Omar Albagha (8977856)Mohamed A Elrayess (11843435)Biological sciencesGeneticsBiomedical and clinical sciencesImmunologyMedical biochemistry and metabolomicsN-Lactoyl amino acidsmGWASPheWASMetabolomics<p dir="ltr">N-lactoyl-amino acids (Lac-AA) are emerging as important metabolites with diverse physiological roles. This study integrates metabolomics and genomics to investigate the genetic determinants and clinical relevance of three Lac-AA: N-Lactoyl phenylalanine (Lac-Phe), N-Lactoyl tyrosine (Lac-Tyr), and N-Lactoyl valine (Lac-Tyr). We conducted a metabolome-wide association study (mGWAS) on 2811 participants followed by a phenome-wide association study (PheWAS) and pathway enrichment analysis. Our mGWAS revealed modest genetic contributions to Lac-AA levels, with genome-wide significant loci identified for Lac-Tyr and Lac-Val, but not for Lac-Phe. PheWAS analysis linked these genetic variants to key clinical traits, including white blood cell count, platelet count, and glucose levels. Pathway enrichment highlighted the involvement of Lac-AA in immune-metabolic crosstalk, particularly in inflammation and energy metabolism. These findings suggest that Lac-AA levels are primarily influenced by dynamic metabolic or inflammatory states rather than fixed genetic factors. Our results underscore the potential of Lac-AA as metabolic sensors and biomarkers at the intersection of cellular energy states and systemic inflammation, opening new avenues for research in metabolic and inflammatory disorders.</p><h2 dir="ltr">Other Information</h2><p dir="ltr">Published in: Human Molecular Genetics<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1093/hmg/ddaf152" target="_blank">https://dx.doi.org/10.1093/hmg/ddaf152</a></p>2025-09-28T09:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1093/hmg/ddaf152https://figshare.com/articles/journal_contribution/N-Lactoyl_amino_acids_insights_from_metabolite_genome-wide_association_studies_and_phenome-wide_association_analysis/31168186CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/311681862025-09-28T09:00:00Z
spellingShingle N-Lactoyl amino acids: insights from metabolite genome-wide association studies and phenome-wide association analysis
Asma A Elashi (18464133)
Biological sciences
Genetics
Biomedical and clinical sciences
Immunology
Medical biochemistry and metabolomics
N-Lactoyl amino acids
mGWAS
PheWAS
Metabolomics
status_str publishedVersion
title N-Lactoyl amino acids: insights from metabolite genome-wide association studies and phenome-wide association analysis
title_full N-Lactoyl amino acids: insights from metabolite genome-wide association studies and phenome-wide association analysis
title_fullStr N-Lactoyl amino acids: insights from metabolite genome-wide association studies and phenome-wide association analysis
title_full_unstemmed N-Lactoyl amino acids: insights from metabolite genome-wide association studies and phenome-wide association analysis
title_short N-Lactoyl amino acids: insights from metabolite genome-wide association studies and phenome-wide association analysis
title_sort N-Lactoyl amino acids: insights from metabolite genome-wide association studies and phenome-wide association analysis
topic Biological sciences
Genetics
Biomedical and clinical sciences
Immunology
Medical biochemistry and metabolomics
N-Lactoyl amino acids
mGWAS
PheWAS
Metabolomics