Correction to: Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1

<p dir="ltr">Correction to: Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1 <a href=" https://doi.org/10.1186/s13287-017-0759-z" rel="noreferrer" target="_blank">https://doi.org/10....

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Main Author: Bushra Memon (4792767) (author)
Other Authors: Manale Karam (3619412) (author), Sara Al-Khawaga (4792761) (author), Essam M. Abdelalim (5768072) (author)
Published: 2018
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_version_ 1864513513513287680
author Bushra Memon (4792767)
author2 Manale Karam (3619412)
Sara Al-Khawaga (4792761)
Essam M. Abdelalim (5768072)
author2_role author
author
author
author_facet Bushra Memon (4792767)
Manale Karam (3619412)
Sara Al-Khawaga (4792761)
Essam M. Abdelalim (5768072)
author_role author
dc.creator.none.fl_str_mv Bushra Memon (4792767)
Manale Karam (3619412)
Sara Al-Khawaga (4792761)
Essam M. Abdelalim (5768072)
dc.date.none.fl_str_mv 2018-10-01T00:00:00Z
dc.identifier.none.fl_str_mv 10.1186/s13287-018-1002-2
dc.relation.none.fl_str_mv https://figshare.com/articles/journal_contribution/Correction_to_Enhanced_differentiation_of_human_pluripotent_stem_cells_into_pancreatic_progenitors_co-expressing_PDX1_and_NKX6_1/25919404
dc.rights.none.fl_str_mv CC BY 4.0
info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Biological sciences
Biochemistry and cell biology
Biomedical and clinical sciences
Medical biotechnology
hPSCs
Beta cells
Diabetes
Differentiation
Transcription factors
Pancreatic epithelium
dc.title.none.fl_str_mv Correction to: Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1
dc.type.none.fl_str_mv Text
Journal contribution
info:eu-repo/semantics/publishedVersion
text
contribution to journal
description <p dir="ltr">Correction to: Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1 <a href=" https://doi.org/10.1186/s13287-017-0759-z" rel="noreferrer" target="_blank">https://doi.org/10.1186/s13287-017-0759-z</a>, published online 23 January 2018.</p><p><br></p><ul><li>The original article contains a number of small errors which the authors would like to clarify:</li></ul><ol><li>1)In Fig. 1a of the original article, FGF10 concentration should be 50 ng/ml – as correctly stated in the Methods section – instead of 100 μg/ml.</li><li>2)Also in Fig. 1a of the original article, Activin A and EGF concentrations should both be 100 ng/ml – as correctly stated in the Methods section – instead of 100 μg/ml.</li><li>3)In the Methods section under the sub-heading “<b>Differentiation of human pluripotent stem cells into pancreatic progenitor</b>”, the term, ‘50 ng/ml hFGF10’ should be omitted from the following sentence:</li></ol><blockquote><p dir="ltr">“<i>At the end of stage 3, media were changed to DMEM supplemented with 1% vol/vol B27, 0.25 mM vitamin C, 50 ng/ml hFGF10, 50 ng/ml hNOGGIN, 100 ng/ml hEGF, and 10 mM nicotinamide (Sigma, USA) for 4 days of stage 4 treatment for each protocol (Fig. 1a)</i>.”</p></blockquote><p dir="ltr">FGF10 was only added during stages 2 & 3 of differentiation as correctly depicted in Fig. 1a.</p><p dir="ltr">The corrected version of Fig. 1a can be viewed ahead.</p><p dir="ltr">The original article has been corrected.</p><h2>Other Information</h2><p dir="ltr">Published in: Stem Cell Research & Therapy<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1186/s13287-018-1002-2" target="_blank">https://dx.doi.org/10.1186/s13287-018-1002-2</a></p>
eu_rights_str_mv openAccess
id Manara2_d0ecda9e4d45e946179ffe2edde7c277
identifier_str_mv 10.1186/s13287-018-1002-2
network_acronym_str Manara2
network_name_str Manara2
oai_identifier_str oai:figshare.com:article/25919404
publishDate 2018
repository.mail.fl_str_mv
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rights_invalid_str_mv CC BY 4.0
spelling Correction to: Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1Bushra Memon (4792767)Manale Karam (3619412)Sara Al-Khawaga (4792761)Essam M. Abdelalim (5768072)Biological sciencesBiochemistry and cell biologyBiomedical and clinical sciencesMedical biotechnologyhPSCsBeta cellsDiabetesDifferentiationTranscription factorsPancreatic epithelium<p dir="ltr">Correction to: Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1 <a href=" https://doi.org/10.1186/s13287-017-0759-z" rel="noreferrer" target="_blank">https://doi.org/10.1186/s13287-017-0759-z</a>, published online 23 January 2018.</p><p><br></p><ul><li>The original article contains a number of small errors which the authors would like to clarify:</li></ul><ol><li>1)In Fig. 1a of the original article, FGF10 concentration should be 50 ng/ml – as correctly stated in the Methods section – instead of 100 μg/ml.</li><li>2)Also in Fig. 1a of the original article, Activin A and EGF concentrations should both be 100 ng/ml – as correctly stated in the Methods section – instead of 100 μg/ml.</li><li>3)In the Methods section under the sub-heading “<b>Differentiation of human pluripotent stem cells into pancreatic progenitor</b>”, the term, ‘50 ng/ml hFGF10’ should be omitted from the following sentence:</li></ol><blockquote><p dir="ltr">“<i>At the end of stage 3, media were changed to DMEM supplemented with 1% vol/vol B27, 0.25 mM vitamin C, 50 ng/ml hFGF10, 50 ng/ml hNOGGIN, 100 ng/ml hEGF, and 10 mM nicotinamide (Sigma, USA) for 4 days of stage 4 treatment for each protocol (Fig. 1a)</i>.”</p></blockquote><p dir="ltr">FGF10 was only added during stages 2 & 3 of differentiation as correctly depicted in Fig. 1a.</p><p dir="ltr">The corrected version of Fig. 1a can be viewed ahead.</p><p dir="ltr">The original article has been corrected.</p><h2>Other Information</h2><p dir="ltr">Published in: Stem Cell Research & Therapy<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1186/s13287-018-1002-2" target="_blank">https://dx.doi.org/10.1186/s13287-018-1002-2</a></p>2018-10-01T00:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1186/s13287-018-1002-2https://figshare.com/articles/journal_contribution/Correction_to_Enhanced_differentiation_of_human_pluripotent_stem_cells_into_pancreatic_progenitors_co-expressing_PDX1_and_NKX6_1/25919404CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/259194042018-10-01T00:00:00Z
spellingShingle Correction to: Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1
Bushra Memon (4792767)
Biological sciences
Biochemistry and cell biology
Biomedical and clinical sciences
Medical biotechnology
hPSCs
Beta cells
Diabetes
Differentiation
Transcription factors
Pancreatic epithelium
status_str publishedVersion
title Correction to: Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1
title_full Correction to: Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1
title_fullStr Correction to: Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1
title_full_unstemmed Correction to: Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1
title_short Correction to: Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1
title_sort Correction to: Enhanced differentiation of human pluripotent stem cells into pancreatic progenitors co-expressing PDX1 and NKX6.1
topic Biological sciences
Biochemistry and cell biology
Biomedical and clinical sciences
Medical biotechnology
hPSCs
Beta cells
Diabetes
Differentiation
Transcription factors
Pancreatic epithelium