Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells
<p>The human pluripotent stem cell (hPSC) differentiation has allowed for the generation of in vitro models to study human diseases in a dish. This protocol describes the generation of keratinocyte-like cells from hPSCs in chemically defined media. Treating hPSCs with retinoic acid and BMP4 in...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | |
| منشور في: |
2022
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| الموضوعات: | |
| الوسوم: |
إضافة وسم
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| _version_ | 1864513519259484160 |
|---|---|
| author | Gowher Ali (14152593) |
| author2 | Essam M. Abdelalim (5768072) |
| author2_role | author |
| author_facet | Gowher Ali (14152593) Essam M. Abdelalim (5768072) |
| author_role | author |
| dc.creator.none.fl_str_mv | Gowher Ali (14152593) Essam M. Abdelalim (5768072) |
| dc.date.none.fl_str_mv | 2022-09-16T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1016/j.xpro.2022.101613 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Directed_differentiation_of_human_pluripotent_stem_cells_into_epidermal_keratinocyte-like_cells/25539613 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Biochemistry and cell biology Genetics Biomedical and clinical sciences Immunology Neurosciences In vitro models Human pluripotent stem cells (hPSCs) Retinoic acid Skin-related diseases Cell culture Cell therapy |
| dc.title.none.fl_str_mv | Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p>The human pluripotent stem cell (hPSC) differentiation has allowed for the generation of in vitro models to study human diseases in a dish. This protocol describes the generation of keratinocyte-like cells from hPSCs in chemically defined media. Treating hPSCs with retinoic acid and BMP4 induced the generation of keratinocyte progenitors, which further differentiated into mature keratinocytes in the presence of calcium. The keratinocytes generated with this protocol could be used to study keratinocyte biology, drug screening, and skin-related diseases. For complete details on the use and execution of this protocol, please refer to Ali et al. (2020).</p><h2>Other Information</h2> <p> Published in: STAR Protocols<br> License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.xpro.2022.101613" target="_blank">https://dx.doi.org/10.1016/j.xpro.2022.101613</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_d14115dc60531323ddd61dd85d758990 |
| identifier_str_mv | 10.1016/j.xpro.2022.101613 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25539613 |
| publishDate | 2022 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cellsGowher Ali (14152593)Essam M. Abdelalim (5768072)Biological sciencesBiochemistry and cell biologyGeneticsBiomedical and clinical sciencesImmunologyNeurosciencesIn vitro modelsHuman pluripotent stem cells (hPSCs)Retinoic acidSkin-related diseasesCell cultureCell therapy<p>The human pluripotent stem cell (hPSC) differentiation has allowed for the generation of in vitro models to study human diseases in a dish. This protocol describes the generation of keratinocyte-like cells from hPSCs in chemically defined media. Treating hPSCs with retinoic acid and BMP4 induced the generation of keratinocyte progenitors, which further differentiated into mature keratinocytes in the presence of calcium. The keratinocytes generated with this protocol could be used to study keratinocyte biology, drug screening, and skin-related diseases. For complete details on the use and execution of this protocol, please refer to Ali et al. (2020).</p><h2>Other Information</h2> <p> Published in: STAR Protocols<br> License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.xpro.2022.101613" target="_blank">https://dx.doi.org/10.1016/j.xpro.2022.101613</a></p>2022-09-16T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1016/j.xpro.2022.101613https://figshare.com/articles/journal_contribution/Directed_differentiation_of_human_pluripotent_stem_cells_into_epidermal_keratinocyte-like_cells/25539613CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/255396132022-09-16T03:00:00Z |
| spellingShingle | Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells Gowher Ali (14152593) Biological sciences Biochemistry and cell biology Genetics Biomedical and clinical sciences Immunology Neurosciences In vitro models Human pluripotent stem cells (hPSCs) Retinoic acid Skin-related diseases Cell culture Cell therapy |
| status_str | publishedVersion |
| title | Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells |
| title_full | Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells |
| title_fullStr | Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells |
| title_full_unstemmed | Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells |
| title_short | Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells |
| title_sort | Directed differentiation of human pluripotent stem cells into epidermal keratinocyte-like cells |
| topic | Biological sciences Biochemistry and cell biology Genetics Biomedical and clinical sciences Immunology Neurosciences In vitro models Human pluripotent stem cells (hPSCs) Retinoic acid Skin-related diseases Cell culture Cell therapy |