Comparison of Serum Amyloid A Measurements in Equine Synovial Fluid With Routine Diagnostic Methods to Detect Synovial Infection in a Clinical Environment
<p dir="ltr">Synovial fluid analysis is utilized to diagnose septic synovitis. However, not all cases are clearly and rapidly discernible with the diagnostic tools available in the laboratory. Serum amyloid A (SAA), an acute phase protein, has been shown to be elevated in synovial fl...
محفوظ في:
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| مؤلفون آخرون: | , , , , , |
| منشور في: |
2019
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| الموضوعات: | |
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إضافة وسم
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| _version_ | 1864513523079446528 |
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| author | John David Stack (18131692) |
| author2 | Matthieu Cousty (18131695) Emma Steele (18131698) Ian Handel (330129) Antoine Lechartier (7325921) Tatiana Vinardell (530336) Florent David (7932980) |
| author2_role | author author author author author author |
| author_facet | John David Stack (18131692) Matthieu Cousty (18131695) Emma Steele (18131698) Ian Handel (330129) Antoine Lechartier (7325921) Tatiana Vinardell (530336) Florent David (7932980) |
| author_role | author |
| dc.creator.none.fl_str_mv | John David Stack (18131692) Matthieu Cousty (18131695) Emma Steele (18131698) Ian Handel (330129) Antoine Lechartier (7325921) Tatiana Vinardell (530336) Florent David (7932980) |
| dc.date.none.fl_str_mv | 2019-10-01T00:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3389/fvets.2019.00325 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Comparison_of_Serum_Amyloid_A_Measurements_in_Equine_Synovial_Fluid_With_Routine_Diagnostic_Methods_to_Detect_Synovial_Infection_in_a_Clinical_Environment/25376431 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Agricultural, veterinary and food sciences Veterinary sciences horse septic synovitis serum amyloid A specificity sensitivity infection arthritis |
| dc.title.none.fl_str_mv | Comparison of Serum Amyloid A Measurements in Equine Synovial Fluid With Routine Diagnostic Methods to Detect Synovial Infection in a Clinical Environment |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Synovial fluid analysis is utilized to diagnose septic synovitis. However, not all cases are clearly and rapidly discernible with the diagnostic tools available in the laboratory. Serum amyloid A (SAA), an acute phase protein, has been shown to be elevated in synovial fluid from inflamed synovial structures. The goal of this study is to describe the correlation between two diagnostic tests measuring equine SAA levels in septic and non-septic synovial structures and to understand the correlation between an elevated SAA result and synovial sepsis. Prospective estimation of sensitivity (Se) and specificity (Sp) of two tests, handheld and ELISA, measuring SAA in synovial fluid was completed in 62 horses presented with injured synovial structures. The comparison was made to a reference diagnosis based on white cell count, percentage of neutrophils, intracellular bacteria and bacterial culture on synovial fluid. Handheld test levels were classified as: 4 lines visible—SAA level negative; 3 lines visible—SAA level mild; 2 lines visible—SAA level moderate; and 1 line visible—SAA level severe and compared to the numerical value obtained with ELISA test. The ELISA SAA test had an area under the curve of 0.88 (0.78–0.98). An ELISA cut-off of 23.95 μg/mL maximized Se and Sp. This cutoff gave a Se of 0.93 (0.66–1.00) and Sp of 0.77 (0.63–0.88). The handheld test was highly correlated with the ELISA SAA test (Spearman rank correlation 0.96) and at a cutoff of moderate or higher for positive results gave identical Se and Sp. Se and Sp of synovial fluid SAA are very reliable when clinical signs of synovitis are present for >6 h. This test, in conjunction with traditional methods, can assist practitioners to rapidly diagnose and expedite appropriate intervention of synovial sepsis.</p><h2>Other Information</h2><p dir="ltr">Published in: Frontiers in Veterinary Science<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3389/fvets.2019.00325" target="_blank">https://dx.doi.org/10.3389/fvets.2019.00325</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_d150a174dbe761b5630dbd70f1df2319 |
| identifier_str_mv | 10.3389/fvets.2019.00325 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25376431 |
| publishDate | 2019 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Comparison of Serum Amyloid A Measurements in Equine Synovial Fluid With Routine Diagnostic Methods to Detect Synovial Infection in a Clinical EnvironmentJohn David Stack (18131692)Matthieu Cousty (18131695)Emma Steele (18131698)Ian Handel (330129)Antoine Lechartier (7325921)Tatiana Vinardell (530336)Florent David (7932980)Agricultural, veterinary and food sciencesVeterinary scienceshorsesepticsynovitisserum amyloid Aspecificitysensitivityinfectionarthritis<p dir="ltr">Synovial fluid analysis is utilized to diagnose septic synovitis. However, not all cases are clearly and rapidly discernible with the diagnostic tools available in the laboratory. Serum amyloid A (SAA), an acute phase protein, has been shown to be elevated in synovial fluid from inflamed synovial structures. The goal of this study is to describe the correlation between two diagnostic tests measuring equine SAA levels in septic and non-septic synovial structures and to understand the correlation between an elevated SAA result and synovial sepsis. Prospective estimation of sensitivity (Se) and specificity (Sp) of two tests, handheld and ELISA, measuring SAA in synovial fluid was completed in 62 horses presented with injured synovial structures. The comparison was made to a reference diagnosis based on white cell count, percentage of neutrophils, intracellular bacteria and bacterial culture on synovial fluid. Handheld test levels were classified as: 4 lines visible—SAA level negative; 3 lines visible—SAA level mild; 2 lines visible—SAA level moderate; and 1 line visible—SAA level severe and compared to the numerical value obtained with ELISA test. The ELISA SAA test had an area under the curve of 0.88 (0.78–0.98). An ELISA cut-off of 23.95 μg/mL maximized Se and Sp. This cutoff gave a Se of 0.93 (0.66–1.00) and Sp of 0.77 (0.63–0.88). The handheld test was highly correlated with the ELISA SAA test (Spearman rank correlation 0.96) and at a cutoff of moderate or higher for positive results gave identical Se and Sp. Se and Sp of synovial fluid SAA are very reliable when clinical signs of synovitis are present for >6 h. This test, in conjunction with traditional methods, can assist practitioners to rapidly diagnose and expedite appropriate intervention of synovial sepsis.</p><h2>Other Information</h2><p dir="ltr">Published in: Frontiers in Veterinary Science<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3389/fvets.2019.00325" target="_blank">https://dx.doi.org/10.3389/fvets.2019.00325</a></p>2019-10-01T00:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3389/fvets.2019.00325https://figshare.com/articles/journal_contribution/Comparison_of_Serum_Amyloid_A_Measurements_in_Equine_Synovial_Fluid_With_Routine_Diagnostic_Methods_to_Detect_Synovial_Infection_in_a_Clinical_Environment/25376431CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/253764312019-10-01T00:00:00Z |
| spellingShingle | Comparison of Serum Amyloid A Measurements in Equine Synovial Fluid With Routine Diagnostic Methods to Detect Synovial Infection in a Clinical Environment John David Stack (18131692) Agricultural, veterinary and food sciences Veterinary sciences horse septic synovitis serum amyloid A specificity sensitivity infection arthritis |
| status_str | publishedVersion |
| title | Comparison of Serum Amyloid A Measurements in Equine Synovial Fluid With Routine Diagnostic Methods to Detect Synovial Infection in a Clinical Environment |
| title_full | Comparison of Serum Amyloid A Measurements in Equine Synovial Fluid With Routine Diagnostic Methods to Detect Synovial Infection in a Clinical Environment |
| title_fullStr | Comparison of Serum Amyloid A Measurements in Equine Synovial Fluid With Routine Diagnostic Methods to Detect Synovial Infection in a Clinical Environment |
| title_full_unstemmed | Comparison of Serum Amyloid A Measurements in Equine Synovial Fluid With Routine Diagnostic Methods to Detect Synovial Infection in a Clinical Environment |
| title_short | Comparison of Serum Amyloid A Measurements in Equine Synovial Fluid With Routine Diagnostic Methods to Detect Synovial Infection in a Clinical Environment |
| title_sort | Comparison of Serum Amyloid A Measurements in Equine Synovial Fluid With Routine Diagnostic Methods to Detect Synovial Infection in a Clinical Environment |
| topic | Agricultural, veterinary and food sciences Veterinary sciences horse septic synovitis serum amyloid A specificity sensitivity infection arthritis |