Analysis of Resistance to Antimicrobials and Presence of Virulence/Stress Response Genes in Campylobacter Isolates from Patients with Severe Diarrhoea
<p dir="ltr"><i>Campylobacter </i>infections are a major cause of diarrhoea world-wide and two of the antimicrobials used for their control (erythromycin and ciprofloxacin) have been losing efficacy in recent years. In a sample of 174 genotyped isolates from the stools of...
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2015
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| _version_ | 1864513511379435520 |
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| author | Haitham Ghunaim (703817) |
| author2 | Jerzy M. Behnke (7571882) Idil Aigha (703818) Aarti Sharma (38717) Sanjay H. Doiphode (14150817) Anand Deshmukh (703820) Marawan M. Abu-Madi (18806905) |
| author2_role | author author author author author author |
| author_facet | Haitham Ghunaim (703817) Jerzy M. Behnke (7571882) Idil Aigha (703818) Aarti Sharma (38717) Sanjay H. Doiphode (14150817) Anand Deshmukh (703820) Marawan M. Abu-Madi (18806905) |
| author_role | author |
| dc.creator.none.fl_str_mv | Haitham Ghunaim (703817) Jerzy M. Behnke (7571882) Idil Aigha (703818) Aarti Sharma (38717) Sanjay H. Doiphode (14150817) Anand Deshmukh (703820) Marawan M. Abu-Madi (18806905) |
| dc.date.none.fl_str_mv | 2015-03-17T06:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pone.0119268 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Analysis_of_Resistance_to_Antimicrobials_and_Presence_of_Virulence_Stress_Response_Genes_in_Campylobacter_Isolates_from_Patients_with_Severe_Diarrhoea/26018275 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Medical microbiology Antimicrobial resistance Campylobacter Virulence factors Erythromycin Diarrhea Antimicrobials Bacterial pathogens Drug interactions |
| dc.title.none.fl_str_mv | Analysis of Resistance to Antimicrobials and Presence of Virulence/Stress Response Genes in Campylobacter Isolates from Patients with Severe Diarrhoea |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr"><i>Campylobacter </i>infections are a major cause of diarrhoea world-wide and two of the antimicrobials used for their control (erythromycin and ciprofloxacin) have been losing efficacy in recent years. In a sample of 174 genotyped isolates from the stools of patients with severe diarrhoea in Qatar, collected between 2005 and 2012, 63.2% showed resistance to ciprofloxacin, 8.6% to erythromycin, 0.57% to chloramphenicol and all were sensitive to gentamycin. While 33.9% of isolates were sensitive to all four antimicrobials, 59.8% were resistant to at least one, 6.3% were resistant to two and none showed resistance to three antimicrobials. There was no host sex- or age-dependence among isolates resistant to ciprofloxacin and erythromycin and no significant variation was found with the region of origin of the patients. All isolates were screened for the presence of 3 virulence factors (<i>ciaB, cadF</i> and <i>cdtB</i>) and two stress-response factors (<i>htrB</i> and <i>clpP</i>), all of which were present in more than 50% of the isolates. Host sex-, age- and region of origin-dependent variations in prevalence were found for some of these factors. Data analysis for the combination of virulence factors and their effect on antimicrobial resistance indicated that the prevalence of resistance to both erythromycin and ciprofloxacin was higher in isolates harbouring <i>ciaB </i>but not <i>clpP</i>. Prevalence of resistance to ciprofloxacin was similar in clpP positive and negative isolates also possessing htrB, while for <i>htrB</i>-negative isolates prevalence was higher in the absence of <i>clpP</i>. These results are discussed and their implications are highlighted.</p><h2>Other Information</h2><p dir="ltr">Published in: PLOS ONE<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1371/journal.pone.0119268" target="_blank">https://dx.doi.org/10.1371/journal.pone.0119268</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_d17212697586978fc458bb1c45ba1cc9 |
| identifier_str_mv | 10.1371/journal.pone.0119268 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/26018275 |
| publishDate | 2015 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Analysis of Resistance to Antimicrobials and Presence of Virulence/Stress Response Genes in Campylobacter Isolates from Patients with Severe DiarrhoeaHaitham Ghunaim (703817)Jerzy M. Behnke (7571882)Idil Aigha (703818)Aarti Sharma (38717)Sanjay H. Doiphode (14150817)Anand Deshmukh (703820)Marawan M. Abu-Madi (18806905)Biomedical and clinical sciencesMedical microbiologyAntimicrobial resistanceCampylobacterVirulence factorsErythromycinDiarrheaAntimicrobialsBacterial pathogensDrug interactions<p dir="ltr"><i>Campylobacter </i>infections are a major cause of diarrhoea world-wide and two of the antimicrobials used for their control (erythromycin and ciprofloxacin) have been losing efficacy in recent years. In a sample of 174 genotyped isolates from the stools of patients with severe diarrhoea in Qatar, collected between 2005 and 2012, 63.2% showed resistance to ciprofloxacin, 8.6% to erythromycin, 0.57% to chloramphenicol and all were sensitive to gentamycin. While 33.9% of isolates were sensitive to all four antimicrobials, 59.8% were resistant to at least one, 6.3% were resistant to two and none showed resistance to three antimicrobials. There was no host sex- or age-dependence among isolates resistant to ciprofloxacin and erythromycin and no significant variation was found with the region of origin of the patients. All isolates were screened for the presence of 3 virulence factors (<i>ciaB, cadF</i> and <i>cdtB</i>) and two stress-response factors (<i>htrB</i> and <i>clpP</i>), all of which were present in more than 50% of the isolates. Host sex-, age- and region of origin-dependent variations in prevalence were found for some of these factors. Data analysis for the combination of virulence factors and their effect on antimicrobial resistance indicated that the prevalence of resistance to both erythromycin and ciprofloxacin was higher in isolates harbouring <i>ciaB </i>but not <i>clpP</i>. Prevalence of resistance to ciprofloxacin was similar in clpP positive and negative isolates also possessing htrB, while for <i>htrB</i>-negative isolates prevalence was higher in the absence of <i>clpP</i>. These results are discussed and their implications are highlighted.</p><h2>Other Information</h2><p dir="ltr">Published in: PLOS ONE<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1371/journal.pone.0119268" target="_blank">https://dx.doi.org/10.1371/journal.pone.0119268</a></p>2015-03-17T06:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1371/journal.pone.0119268https://figshare.com/articles/journal_contribution/Analysis_of_Resistance_to_Antimicrobials_and_Presence_of_Virulence_Stress_Response_Genes_in_Campylobacter_Isolates_from_Patients_with_Severe_Diarrhoea/26018275CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/260182752015-03-17T06:00:00Z |
| spellingShingle | Analysis of Resistance to Antimicrobials and Presence of Virulence/Stress Response Genes in Campylobacter Isolates from Patients with Severe Diarrhoea Haitham Ghunaim (703817) Biomedical and clinical sciences Medical microbiology Antimicrobial resistance Campylobacter Virulence factors Erythromycin Diarrhea Antimicrobials Bacterial pathogens Drug interactions |
| status_str | publishedVersion |
| title | Analysis of Resistance to Antimicrobials and Presence of Virulence/Stress Response Genes in Campylobacter Isolates from Patients with Severe Diarrhoea |
| title_full | Analysis of Resistance to Antimicrobials and Presence of Virulence/Stress Response Genes in Campylobacter Isolates from Patients with Severe Diarrhoea |
| title_fullStr | Analysis of Resistance to Antimicrobials and Presence of Virulence/Stress Response Genes in Campylobacter Isolates from Patients with Severe Diarrhoea |
| title_full_unstemmed | Analysis of Resistance to Antimicrobials and Presence of Virulence/Stress Response Genes in Campylobacter Isolates from Patients with Severe Diarrhoea |
| title_short | Analysis of Resistance to Antimicrobials and Presence of Virulence/Stress Response Genes in Campylobacter Isolates from Patients with Severe Diarrhoea |
| title_sort | Analysis of Resistance to Antimicrobials and Presence of Virulence/Stress Response Genes in Campylobacter Isolates from Patients with Severe Diarrhoea |
| topic | Biomedical and clinical sciences Medical microbiology Antimicrobial resistance Campylobacter Virulence factors Erythromycin Diarrhea Antimicrobials Bacterial pathogens Drug interactions |