<i>Asterarcys quadricellulare</i> (Chlorophyceae) protects H9c2 cardiomyoblasts from H<sub>2</sub>O<sub>2</sub>-induced oxidative stress
<p dir="ltr">Oxidative stress has recently been identified as an important mediator of cardiovascular diseases. The need to find efficient antioxidant molecules is essential in the disease’s prevention. Therefore, the present study aimed to evaluate the potential of microalgae bioact...
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2022
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| author | Imen Saadaoui (14159025) |
| author2 | Touria Bounnit (16936899) Fatima Mraiche (715733) Jensa M. Joseph (21623756) Maroua Cherif (14153388) Hareb Al-Jabri (17092903) |
| author2_role | author author author author author |
| author_facet | Imen Saadaoui (14159025) Touria Bounnit (16936899) Fatima Mraiche (715733) Jensa M. Joseph (21623756) Maroua Cherif (14153388) Hareb Al-Jabri (17092903) |
| author_role | author |
| dc.creator.none.fl_str_mv | Imen Saadaoui (14159025) Touria Bounnit (16936899) Fatima Mraiche (715733) Jensa M. Joseph (21623756) Maroua Cherif (14153388) Hareb Al-Jabri (17092903) |
| dc.date.none.fl_str_mv | 2022-12-30T09:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1007/s11010-022-04626-7 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/_i_Asterarcys_quadricellulare_i_Chlorophyceae_protects_H9c2_cardiomyoblasts_from_H_sub_2_sub_O_sub_2_sub_-induced_oxidative_stress/29435963 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Cardiovascular medicine and haematology Pharmacology and pharmaceutical sciences Antioxidant Asterarcys quadricellulare Cardiovascular diseases Caspase-3 High value products Reactive Oxygen Species |
| dc.title.none.fl_str_mv | <i>Asterarcys quadricellulare</i> (Chlorophyceae) protects H9c2 cardiomyoblasts from H<sub>2</sub>O<sub>2</sub>-induced oxidative stress |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Oxidative stress has recently been identified as an important mediator of cardiovascular diseases. The need to find efficient antioxidant molecules is essential in the disease’s prevention. Therefore, the present study aimed to evaluate the potential of microalgae bioactive in protecting H9c2 cardiomyoblasts from H<sub>2</sub>O<sub>2</sub>-induced oxidative stress. Four microalgal species were investigated for their antioxidant capacity. A qualitative assessment of oxidative stress in H9c2 cardiomyoblasts stained with DCFH-DA, treated with the highly active microalgae extracts, was performed. The protein expression of total caspase-3 was also examined to investigate whether the extract protects H9c2 cardimyoblasts from H2O2-induced apoptosis. High antioxidant activity was observed for the hexanoic extracts after 10 days of cultivation. <i>Asterarcys quadricellulare</i> exhibited the highest antioxidant capacity of 110.59 ± 1.75 mg TE g<sup>−1</sup> dry weight and was tested against H9c2 cardiomyoblasts, which were initially subjected to H<sub>2</sub>O<sub>2</sub>-induced oxidative stress. This hexanoic extract protected against H<sub>2</sub>O<sub>2</sub> induced oxidative stress with a similar scavenging capacity as N-Acetylcysteine. Furthermore, total caspase-3 was increased following treatment with the hexanoic extract, suggesting that <i>A. quadricellulare</i> also had anti-apoptotic properties. The outcome of our study highlighted the possible use of the local <i>A. quadricellulare</i> strain QUCCCM10 as a natural, safe, and efficient antioxidant to prevent cardiovascular diseases.</p><h2>Other Information</h2><p dir="ltr">Published in: Molecular and Cellular Biochemistry<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1007/s11010-022-04626-7" target="_blank">https://dx.doi.org/10.1007/s11010-022-04626-7</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_d195d73465b57555ea244d5f9b273ac3 |
| identifier_str_mv | 10.1007/s11010-022-04626-7 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/29435963 |
| publishDate | 2022 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | <i>Asterarcys quadricellulare</i> (Chlorophyceae) protects H9c2 cardiomyoblasts from H<sub>2</sub>O<sub>2</sub>-induced oxidative stressImen Saadaoui (14159025)Touria Bounnit (16936899)Fatima Mraiche (715733)Jensa M. Joseph (21623756)Maroua Cherif (14153388)Hareb Al-Jabri (17092903)Biomedical and clinical sciencesCardiovascular medicine and haematologyPharmacology and pharmaceutical sciencesAntioxidantAsterarcys quadricellulareCardiovascular diseasesCaspase-3High value productsReactive Oxygen Species<p dir="ltr">Oxidative stress has recently been identified as an important mediator of cardiovascular diseases. The need to find efficient antioxidant molecules is essential in the disease’s prevention. Therefore, the present study aimed to evaluate the potential of microalgae bioactive in protecting H9c2 cardiomyoblasts from H<sub>2</sub>O<sub>2</sub>-induced oxidative stress. Four microalgal species were investigated for their antioxidant capacity. A qualitative assessment of oxidative stress in H9c2 cardiomyoblasts stained with DCFH-DA, treated with the highly active microalgae extracts, was performed. The protein expression of total caspase-3 was also examined to investigate whether the extract protects H9c2 cardimyoblasts from H2O2-induced apoptosis. High antioxidant activity was observed for the hexanoic extracts after 10 days of cultivation. <i>Asterarcys quadricellulare</i> exhibited the highest antioxidant capacity of 110.59 ± 1.75 mg TE g<sup>−1</sup> dry weight and was tested against H9c2 cardiomyoblasts, which were initially subjected to H<sub>2</sub>O<sub>2</sub>-induced oxidative stress. This hexanoic extract protected against H<sub>2</sub>O<sub>2</sub> induced oxidative stress with a similar scavenging capacity as N-Acetylcysteine. Furthermore, total caspase-3 was increased following treatment with the hexanoic extract, suggesting that <i>A. quadricellulare</i> also had anti-apoptotic properties. The outcome of our study highlighted the possible use of the local <i>A. quadricellulare</i> strain QUCCCM10 as a natural, safe, and efficient antioxidant to prevent cardiovascular diseases.</p><h2>Other Information</h2><p dir="ltr">Published in: Molecular and Cellular Biochemistry<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1007/s11010-022-04626-7" target="_blank">https://dx.doi.org/10.1007/s11010-022-04626-7</a></p>2022-12-30T09:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1007/s11010-022-04626-7https://figshare.com/articles/journal_contribution/_i_Asterarcys_quadricellulare_i_Chlorophyceae_protects_H9c2_cardiomyoblasts_from_H_sub_2_sub_O_sub_2_sub_-induced_oxidative_stress/29435963CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/294359632022-12-30T09:00:00Z |
| spellingShingle | <i>Asterarcys quadricellulare</i> (Chlorophyceae) protects H9c2 cardiomyoblasts from H<sub>2</sub>O<sub>2</sub>-induced oxidative stress Imen Saadaoui (14159025) Biomedical and clinical sciences Cardiovascular medicine and haematology Pharmacology and pharmaceutical sciences Antioxidant Asterarcys quadricellulare Cardiovascular diseases Caspase-3 High value products Reactive Oxygen Species |
| status_str | publishedVersion |
| title | <i>Asterarcys quadricellulare</i> (Chlorophyceae) protects H9c2 cardiomyoblasts from H<sub>2</sub>O<sub>2</sub>-induced oxidative stress |
| title_full | <i>Asterarcys quadricellulare</i> (Chlorophyceae) protects H9c2 cardiomyoblasts from H<sub>2</sub>O<sub>2</sub>-induced oxidative stress |
| title_fullStr | <i>Asterarcys quadricellulare</i> (Chlorophyceae) protects H9c2 cardiomyoblasts from H<sub>2</sub>O<sub>2</sub>-induced oxidative stress |
| title_full_unstemmed | <i>Asterarcys quadricellulare</i> (Chlorophyceae) protects H9c2 cardiomyoblasts from H<sub>2</sub>O<sub>2</sub>-induced oxidative stress |
| title_short | <i>Asterarcys quadricellulare</i> (Chlorophyceae) protects H9c2 cardiomyoblasts from H<sub>2</sub>O<sub>2</sub>-induced oxidative stress |
| title_sort | <i>Asterarcys quadricellulare</i> (Chlorophyceae) protects H9c2 cardiomyoblasts from H<sub>2</sub>O<sub>2</sub>-induced oxidative stress |
| topic | Biomedical and clinical sciences Cardiovascular medicine and haematology Pharmacology and pharmaceutical sciences Antioxidant Asterarcys quadricellulare Cardiovascular diseases Caspase-3 High value products Reactive Oxygen Species |