Chondrocyte De-Differentiation: Biophysical Cues to Nuclear Alterations
<div><p>Autologous chondrocyte implantation (ACI) is a cell therapy to repair cartilage defects. In ACI a biopsy is taken from a non-load bearing area of the knee and expanded in-vitro. The expansion process provides the benefit of generating a large number of cells required for implanta...
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2022
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| _version_ | 1864513519252144128 |
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| author | Noor A. Al-Maslamani (17316925) |
| author2 | Rachel Oldershaw (18306601) Simon Tew (18306604) Jude Curran (18306607) Pieter D’Hooghe (3530984) Kazuhiro Yamamoto (115929) Henning F. Horn (14152938) |
| author2_role | author author author author author author |
| author_facet | Noor A. Al-Maslamani (17316925) Rachel Oldershaw (18306601) Simon Tew (18306604) Jude Curran (18306607) Pieter D’Hooghe (3530984) Kazuhiro Yamamoto (115929) Henning F. Horn (14152938) |
| author_role | author |
| dc.creator.none.fl_str_mv | Noor A. Al-Maslamani (17316925) Rachel Oldershaw (18306601) Simon Tew (18306604) Jude Curran (18306607) Pieter D’Hooghe (3530984) Kazuhiro Yamamoto (115929) Henning F. Horn (14152938) |
| dc.date.none.fl_str_mv | 2022-12-12T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3390/cells11244011 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Chondrocyte_De-Differentiation_Biophysical_Cues_to_Nuclear_Alterations/25539643 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Cardiovascular medicine and haematology autologous chondrocyte implantation de-differentiation re-differentiation chondrocyte mechanobiology biophysics two-dimensional culture (2D) three-dimensional culture (3D) |
| dc.title.none.fl_str_mv | Chondrocyte De-Differentiation: Biophysical Cues to Nuclear Alterations |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <div><p>Autologous chondrocyte implantation (ACI) is a cell therapy to repair cartilage defects. In ACI a biopsy is taken from a non-load bearing area of the knee and expanded in-vitro. The expansion process provides the benefit of generating a large number of cells required for implantation; however, during the expansion these cells de-differentiate and lose their chondrocyte phenotype. In this review we focus on examining the de-differentiation phenotype from a mechanobiology and biophysical perspective, highlighting some of the nuclear mechanics and chromatin changes in chondrocytes seen during the expansion process and how this relates to the gene expression profile. We propose that manipulating chondrocyte nuclear architecture and chromatin organization will highlight mechanisms that will help to preserve the chondrocyte phenotype.</p><p> </p></div><h2>Other Information</h2> <p> Published in: Cells<br> License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/cells11244011" target="_blank">https://dx.doi.org/10.3390/cells11244011</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_d4e55619ee25ac07fd1ef6aab7dd70b3 |
| identifier_str_mv | 10.3390/cells11244011 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25539643 |
| publishDate | 2022 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Chondrocyte De-Differentiation: Biophysical Cues to Nuclear AlterationsNoor A. Al-Maslamani (17316925)Rachel Oldershaw (18306601)Simon Tew (18306604)Jude Curran (18306607)Pieter D’Hooghe (3530984)Kazuhiro Yamamoto (115929)Henning F. Horn (14152938)Biomedical and clinical sciencesCardiovascular medicine and haematologyautologous chondrocyte implantationde-differentiationre-differentiationchondrocytemechanobiologybiophysicstwo-dimensional culture (2D)three-dimensional culture (3D)<div><p>Autologous chondrocyte implantation (ACI) is a cell therapy to repair cartilage defects. In ACI a biopsy is taken from a non-load bearing area of the knee and expanded in-vitro. The expansion process provides the benefit of generating a large number of cells required for implantation; however, during the expansion these cells de-differentiate and lose their chondrocyte phenotype. In this review we focus on examining the de-differentiation phenotype from a mechanobiology and biophysical perspective, highlighting some of the nuclear mechanics and chromatin changes in chondrocytes seen during the expansion process and how this relates to the gene expression profile. We propose that manipulating chondrocyte nuclear architecture and chromatin organization will highlight mechanisms that will help to preserve the chondrocyte phenotype.</p><p> </p></div><h2>Other Information</h2> <p> Published in: Cells<br> License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/cells11244011" target="_blank">https://dx.doi.org/10.3390/cells11244011</a></p>2022-12-12T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3390/cells11244011https://figshare.com/articles/journal_contribution/Chondrocyte_De-Differentiation_Biophysical_Cues_to_Nuclear_Alterations/25539643CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/255396432022-12-12T03:00:00Z |
| spellingShingle | Chondrocyte De-Differentiation: Biophysical Cues to Nuclear Alterations Noor A. Al-Maslamani (17316925) Biomedical and clinical sciences Cardiovascular medicine and haematology autologous chondrocyte implantation de-differentiation re-differentiation chondrocyte mechanobiology biophysics two-dimensional culture (2D) three-dimensional culture (3D) |
| status_str | publishedVersion |
| title | Chondrocyte De-Differentiation: Biophysical Cues to Nuclear Alterations |
| title_full | Chondrocyte De-Differentiation: Biophysical Cues to Nuclear Alterations |
| title_fullStr | Chondrocyte De-Differentiation: Biophysical Cues to Nuclear Alterations |
| title_full_unstemmed | Chondrocyte De-Differentiation: Biophysical Cues to Nuclear Alterations |
| title_short | Chondrocyte De-Differentiation: Biophysical Cues to Nuclear Alterations |
| title_sort | Chondrocyte De-Differentiation: Biophysical Cues to Nuclear Alterations |
| topic | Biomedical and clinical sciences Cardiovascular medicine and haematology autologous chondrocyte implantation de-differentiation re-differentiation chondrocyte mechanobiology biophysics two-dimensional culture (2D) three-dimensional culture (3D) |