Rapid generation of purified human RPE from pluripotent stem cells using 2D cultures and lipoprotein uptake-based sorting

Rapid generation of purified human RPE from pluripotent stem cells using 2D cultures and lipoprotein uptake-based sorting

<h3>Background</h3><p dir="ltr">Despite increasing demand, current protocols for human pluripotent stem cell (hPSC)-derived retinal pigment epithelium (RPE) remain time, labor, and cost intensive. Additionally, absence of robust methods for selective RPE purification and...

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Bibliographic Details
Main Author: Fabio Michelet (8396283) (author)
Other Authors: Aishwarya Balasankar (8396286) (author), Nickolas Teo (8396289) (author), Lawrence W. Stanton (6707191) (author), Shweta Singhal (8396292) (author)
Published: 2020
Subjects:
Biological sciences
Biochemistry and cell biology
Biomedical and clinical sciences
Medical biotechnology
Human pluripotent stem cells (hPSC)
Retinal pigment epithelium (RPE)
RPE morphology
VEGF expression
Transepithelial electrical resistance (TEER)
Fluorescence-activated cell sorting (FACS)
Cell replacement therapy
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