STAT3 promotes IFNγ/TNFα‐induced muscle wasting in an NF‐κB‐dependent and IL‐6‐independent manner

<p dir="ltr">Cachexia is a debilitating syndrome characterized by involuntary muscle wasting that is triggered at the late stage of many cancers. While the multifactorial nature of this syndrome and the implication of cytokines such as IL‐6, IFNγ, and TNFα is well established, we sti...

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Main Author: Jennifer F Ma (18629359) (author)
Other Authors: Brenda J Sanchez (18696940) (author), Derek T Hall (18629356) (author), Anne‐Marie K Tremblay (19691713) (author), Sergio Di Marco (387749) (author), Imed‐Eddine Gallouzi (18629386) (author)
Published: 2017
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_version_ 1864513557170749440
author Jennifer F Ma (18629359)
author2 Brenda J Sanchez (18696940)
Derek T Hall (18629356)
Anne‐Marie K Tremblay (19691713)
Sergio Di Marco (387749)
Imed‐Eddine Gallouzi (18629386)
author2_role author
author
author
author
author
author_facet Jennifer F Ma (18629359)
Brenda J Sanchez (18696940)
Derek T Hall (18629356)
Anne‐Marie K Tremblay (19691713)
Sergio Di Marco (387749)
Imed‐Eddine Gallouzi (18629386)
author_role author
dc.creator.none.fl_str_mv Jennifer F Ma (18629359)
Brenda J Sanchez (18696940)
Derek T Hall (18629356)
Anne‐Marie K Tremblay (19691713)
Sergio Di Marco (387749)
Imed‐Eddine Gallouzi (18629386)
dc.date.none.fl_str_mv 2017-03-06T03:00:00Z
dc.identifier.none.fl_str_mv 10.15252/emmm.201607052
dc.relation.none.fl_str_mv https://figshare.com/articles/journal_contribution/STAT__3_promotes__IFN____TNF__induced_muscle_wasting_in_an__NF__B_dependent_and__IL__6_independent_manner/27050752
dc.rights.none.fl_str_mv CC BY 4.0
info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Biomedical and clinical sciences
Immunology
Oncology and carcinogenesis
inflammation
iNOS
muscle wasting
NF-jB
STAT3
dc.title.none.fl_str_mv STAT3 promotes IFNγ/TNFα‐induced muscle wasting in an NF‐κB‐dependent and IL‐6‐independent manner
dc.type.none.fl_str_mv Text
Journal contribution
info:eu-repo/semantics/publishedVersion
text
contribution to journal
description <p dir="ltr">Cachexia is a debilitating syndrome characterized by involuntary muscle wasting that is triggered at the late stage of many cancers. While the multifactorial nature of this syndrome and the implication of cytokines such as IL‐6, IFNγ, and TNFα is well established, we still do not know how various effector pathways collaborate together to trigger muscle atrophy. Here, we show that IFNγ/TNFα promotes the phosphorylation of STAT3 on Y705 residue in the cytoplasm of muscle fibers by activating JAK kinases. Unexpectedly, this effect occurs both <i>in vitro</i> and <i>in vivo</i> independently of IL‐6, which is considered as one of the main triggers of STAT3‐mediated muscle wasting. pY‐STAT3 forms a complex with NF‐κB that is rapidly imported to the nucleus where it is recruited to the promoter of the <i>iN</i><i>os</i> gene to activate the iNOS/NO pathway, a well‐known downstream effector of IFNγ/TNFα‐induced muscle loss. Together, these findings show that STAT3 and NF‐κB respond to the same upstream signal and cooperate to promote the expression of pro‐cachectic genes, the identification of which could provide effective targets to combat this deadly syndrome.</p><h2>Other Information</h2><p dir="ltr">Published in: EMBO Molecular Medicine<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.15252/emmm.201607052" target="_blank">https://dx.doi.org/10.15252/emmm.201607052</a></p>
eu_rights_str_mv openAccess
id Manara2_d6aac7eff8efd398520e9f8e41630985
identifier_str_mv 10.15252/emmm.201607052
network_acronym_str Manara2
network_name_str Manara2
oai_identifier_str oai:figshare.com:article/27050752
publishDate 2017
repository.mail.fl_str_mv
repository.name.fl_str_mv
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rights_invalid_str_mv CC BY 4.0
spelling STAT3 promotes IFNγ/TNFα‐induced muscle wasting in an NF‐κB‐dependent and IL‐6‐independent mannerJennifer F Ma (18629359)Brenda J Sanchez (18696940)Derek T Hall (18629356)Anne‐Marie K Tremblay (19691713)Sergio Di Marco (387749)Imed‐Eddine Gallouzi (18629386)Biomedical and clinical sciencesImmunologyOncology and carcinogenesisinflammationiNOSmuscle wastingNF-jBSTAT3<p dir="ltr">Cachexia is a debilitating syndrome characterized by involuntary muscle wasting that is triggered at the late stage of many cancers. While the multifactorial nature of this syndrome and the implication of cytokines such as IL‐6, IFNγ, and TNFα is well established, we still do not know how various effector pathways collaborate together to trigger muscle atrophy. Here, we show that IFNγ/TNFα promotes the phosphorylation of STAT3 on Y705 residue in the cytoplasm of muscle fibers by activating JAK kinases. Unexpectedly, this effect occurs both <i>in vitro</i> and <i>in vivo</i> independently of IL‐6, which is considered as one of the main triggers of STAT3‐mediated muscle wasting. pY‐STAT3 forms a complex with NF‐κB that is rapidly imported to the nucleus where it is recruited to the promoter of the <i>iN</i><i>os</i> gene to activate the iNOS/NO pathway, a well‐known downstream effector of IFNγ/TNFα‐induced muscle loss. Together, these findings show that STAT3 and NF‐κB respond to the same upstream signal and cooperate to promote the expression of pro‐cachectic genes, the identification of which could provide effective targets to combat this deadly syndrome.</p><h2>Other Information</h2><p dir="ltr">Published in: EMBO Molecular Medicine<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.15252/emmm.201607052" target="_blank">https://dx.doi.org/10.15252/emmm.201607052</a></p>2017-03-06T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.15252/emmm.201607052https://figshare.com/articles/journal_contribution/STAT__3_promotes__IFN____TNF__induced_muscle_wasting_in_an__NF__B_dependent_and__IL__6_independent_manner/27050752CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/270507522017-03-06T03:00:00Z
spellingShingle STAT3 promotes IFNγ/TNFα‐induced muscle wasting in an NF‐κB‐dependent and IL‐6‐independent manner
Jennifer F Ma (18629359)
Biomedical and clinical sciences
Immunology
Oncology and carcinogenesis
inflammation
iNOS
muscle wasting
NF-jB
STAT3
status_str publishedVersion
title STAT3 promotes IFNγ/TNFα‐induced muscle wasting in an NF‐κB‐dependent and IL‐6‐independent manner
title_full STAT3 promotes IFNγ/TNFα‐induced muscle wasting in an NF‐κB‐dependent and IL‐6‐independent manner
title_fullStr STAT3 promotes IFNγ/TNFα‐induced muscle wasting in an NF‐κB‐dependent and IL‐6‐independent manner
title_full_unstemmed STAT3 promotes IFNγ/TNFα‐induced muscle wasting in an NF‐κB‐dependent and IL‐6‐independent manner
title_short STAT3 promotes IFNγ/TNFα‐induced muscle wasting in an NF‐κB‐dependent and IL‐6‐independent manner
title_sort STAT3 promotes IFNγ/TNFα‐induced muscle wasting in an NF‐κB‐dependent and IL‐6‐independent manner
topic Biomedical and clinical sciences
Immunology
Oncology and carcinogenesis
inflammation
iNOS
muscle wasting
NF-jB
STAT3