Interleukin-35 and Thymoquinone nanoparticle-based intervention for liver protection against paracetamol-induced liver injury in rats
<p>Paracetamol (PAR) is a commonly used antipyretic and analgesic agent, but its excessive usage can induce liver damage and major health consequences. Interleukin-35 (IL-35) is utilized to treat immunological disorders, intestinal illness, arthritis, allergic disease, hepatitis, and cancer. T...
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| مؤلفون آخرون: | , , , , , |
| منشور في: |
2023
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| _version_ | 1864513535786090496 |
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| author | Maisa Siddiq Abduh (16381125) |
| author2 | Sultan Ayesh Mohammed Saghir (17541381) Naif Ahmed Al-Gabri (17541384) Ahmad Faheem Ahmeda (17541387) Mouaadh Abdelkarim (17541390) Saleh Mohammad Aldaqal (17541393) Mohammed Abdullah Alshawsh (9760803) |
| author2_role | author author author author author author |
| author_facet | Maisa Siddiq Abduh (16381125) Sultan Ayesh Mohammed Saghir (17541381) Naif Ahmed Al-Gabri (17541384) Ahmad Faheem Ahmeda (17541387) Mouaadh Abdelkarim (17541390) Saleh Mohammad Aldaqal (17541393) Mohammed Abdullah Alshawsh (9760803) |
| author_role | author |
| dc.creator.none.fl_str_mv | Maisa Siddiq Abduh (16381125) Sultan Ayesh Mohammed Saghir (17541381) Naif Ahmed Al-Gabri (17541384) Ahmad Faheem Ahmeda (17541387) Mouaadh Abdelkarim (17541390) Saleh Mohammad Aldaqal (17541393) Mohammed Abdullah Alshawsh (9760803) |
| dc.date.none.fl_str_mv | 2023-10-01T12:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1016/j.sjbs.2023.103806 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Interleukin-35_and_Thymoquinone_nanoparticle-based_intervention_for_liver_protection_against_paracetamol-induced_liver_injury_in_rats/24717183 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Pharmacology and pharmaceutical sciences Interleukin-35 Thymoquinone Liver injury Hepatotoxicity Paracetamol |
| dc.title.none.fl_str_mv | Interleukin-35 and Thymoquinone nanoparticle-based intervention for liver protection against paracetamol-induced liver injury in rats |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p>Paracetamol (PAR) is a commonly used antipyretic and analgesic agent, but its excessive usage can induce liver damage and major health consequences. Interleukin-35 (IL-35) is utilized to treat immunological disorders, intestinal illness, arthritis, allergic disease, hepatitis, and cancer. Thymoquinone (THYO) is also effective against a wide range of disorders. Consequently, this study sought out to explore the ameliorative effects of IL-35 and THYO against PAR-induced hepatotoxicity in rats. Sixty male rats were separated into six groups (10 rats/group): I control (0.5 mL NaCl, 0.9%/rat via oral gavage); II (IL-35), and III (TYHO) received intraperitoneal (i.p) injection of IL-35 (200 ng/kg) or THYO (0.5 mg/kg), respectively. Group IV (PAR) received 600 mg/kg of PAR orally; V (PAR + IL-35) and VI (PAR + TYHO); rats received 600 mg/kg of PAR orally and i.p injection of IL-35 (200 ng/kg) or THYO (0.5 mg/kg), respectively. Administration of IL-35 or THYO markedly mitigated the increasing in the levels of liver parameters triggered by PAR and noticeable enhancement of antioxidant and immunological markers were observed. Additionally, IL-35 or THYO decreased TNF-α, NF-κB, IL-10, IL-6 and IFN-γ in contrast to the PAR control group. Moreover, levels of Capase-3, and cytochrome C were significantly reduced by THYO or IL35, while, levels of Bcl-2 were markedly increased. Furthermore, significant downregulation of IL1-β, TNF-α, TGF-β, and Caspas-3 genes, as well as significant upregulation of Bcl-2 and IL-10 expression were detected. In conclusion, IL-35 and THYO insulated liver from PAR toxicity by mitigating oxidative stress, tissue damage, inflammation, and apoptosis.</p><h2>Other Information</h2> <p> Published in: Saudi Journal of Biological Sciences<br> License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.sjbs.2023.103806" target="_blank">https://dx.doi.org/10.1016/j.sjbs.2023.103806</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_df75ef4f50d9c00aea1bea66e41ab311 |
| identifier_str_mv | 10.1016/j.sjbs.2023.103806 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/24717183 |
| publishDate | 2023 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Interleukin-35 and Thymoquinone nanoparticle-based intervention for liver protection against paracetamol-induced liver injury in ratsMaisa Siddiq Abduh (16381125)Sultan Ayesh Mohammed Saghir (17541381)Naif Ahmed Al-Gabri (17541384)Ahmad Faheem Ahmeda (17541387)Mouaadh Abdelkarim (17541390)Saleh Mohammad Aldaqal (17541393)Mohammed Abdullah Alshawsh (9760803)Biomedical and clinical sciencesPharmacology and pharmaceutical sciencesInterleukin-35ThymoquinoneLiver injuryHepatotoxicityParacetamol<p>Paracetamol (PAR) is a commonly used antipyretic and analgesic agent, but its excessive usage can induce liver damage and major health consequences. Interleukin-35 (IL-35) is utilized to treat immunological disorders, intestinal illness, arthritis, allergic disease, hepatitis, and cancer. Thymoquinone (THYO) is also effective against a wide range of disorders. Consequently, this study sought out to explore the ameliorative effects of IL-35 and THYO against PAR-induced hepatotoxicity in rats. Sixty male rats were separated into six groups (10 rats/group): I control (0.5 mL NaCl, 0.9%/rat via oral gavage); II (IL-35), and III (TYHO) received intraperitoneal (i.p) injection of IL-35 (200 ng/kg) or THYO (0.5 mg/kg), respectively. Group IV (PAR) received 600 mg/kg of PAR orally; V (PAR + IL-35) and VI (PAR + TYHO); rats received 600 mg/kg of PAR orally and i.p injection of IL-35 (200 ng/kg) or THYO (0.5 mg/kg), respectively. Administration of IL-35 or THYO markedly mitigated the increasing in the levels of liver parameters triggered by PAR and noticeable enhancement of antioxidant and immunological markers were observed. Additionally, IL-35 or THYO decreased TNF-α, NF-κB, IL-10, IL-6 and IFN-γ in contrast to the PAR control group. Moreover, levels of Capase-3, and cytochrome C were significantly reduced by THYO or IL35, while, levels of Bcl-2 were markedly increased. Furthermore, significant downregulation of IL1-β, TNF-α, TGF-β, and Caspas-3 genes, as well as significant upregulation of Bcl-2 and IL-10 expression were detected. In conclusion, IL-35 and THYO insulated liver from PAR toxicity by mitigating oxidative stress, tissue damage, inflammation, and apoptosis.</p><h2>Other Information</h2> <p> Published in: Saudi Journal of Biological Sciences<br> License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.sjbs.2023.103806" target="_blank">https://dx.doi.org/10.1016/j.sjbs.2023.103806</a></p>2023-10-01T12:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1016/j.sjbs.2023.103806https://figshare.com/articles/journal_contribution/Interleukin-35_and_Thymoquinone_nanoparticle-based_intervention_for_liver_protection_against_paracetamol-induced_liver_injury_in_rats/24717183CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/247171832023-10-01T12:00:00Z |
| spellingShingle | Interleukin-35 and Thymoquinone nanoparticle-based intervention for liver protection against paracetamol-induced liver injury in rats Maisa Siddiq Abduh (16381125) Biomedical and clinical sciences Pharmacology and pharmaceutical sciences Interleukin-35 Thymoquinone Liver injury Hepatotoxicity Paracetamol |
| status_str | publishedVersion |
| title | Interleukin-35 and Thymoquinone nanoparticle-based intervention for liver protection against paracetamol-induced liver injury in rats |
| title_full | Interleukin-35 and Thymoquinone nanoparticle-based intervention for liver protection against paracetamol-induced liver injury in rats |
| title_fullStr | Interleukin-35 and Thymoquinone nanoparticle-based intervention for liver protection against paracetamol-induced liver injury in rats |
| title_full_unstemmed | Interleukin-35 and Thymoquinone nanoparticle-based intervention for liver protection against paracetamol-induced liver injury in rats |
| title_short | Interleukin-35 and Thymoquinone nanoparticle-based intervention for liver protection against paracetamol-induced liver injury in rats |
| title_sort | Interleukin-35 and Thymoquinone nanoparticle-based intervention for liver protection against paracetamol-induced liver injury in rats |
| topic | Biomedical and clinical sciences Pharmacology and pharmaceutical sciences Interleukin-35 Thymoquinone Liver injury Hepatotoxicity Paracetamol |