Atom Probe Tomographic Mapping Directly Reveals the Atomic Distribution of Phosphorus in Resin Embedded Ferritin
<p dir="ltr">Here we report the atomic-scale analysis of biological interfaces within the ferritin protein using atom probe tomography that is facilitated by an advanced specimen preparation approach. Embedding ferritin in an organic polymer resin lacking nitrogen provided chemical c...
محفوظ في:
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| مؤلفون آخرون: | , , , , , |
| منشور في: |
2016
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| _version_ | 1864513556532166656 |
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| author | Daniel E. Perea (1767190) |
| author2 | Jia Liu (41593) Jonah Bartrand (19730746) Quinten Dicken (19730749) S. Theva Thevuthasan (19730752) Nigel D. Browning (1234674) James E. Evans (7485) |
| author2_role | author author author author author author |
| author_facet | Daniel E. Perea (1767190) Jia Liu (41593) Jonah Bartrand (19730746) Quinten Dicken (19730749) S. Theva Thevuthasan (19730752) Nigel D. Browning (1234674) James E. Evans (7485) |
| author_role | author |
| dc.creator.none.fl_str_mv | Daniel E. Perea (1767190) Jia Liu (41593) Jonah Bartrand (19730746) Quinten Dicken (19730749) S. Theva Thevuthasan (19730752) Nigel D. Browning (1234674) James E. Evans (7485) |
| dc.date.none.fl_str_mv | 2016-02-29T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1038/srep22321 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Atom_Probe_Tomographic_Mapping_Directly_Reveals_the_Atomic_Distribution_of_Phosphorus_in_Resin_Embedded_Ferritin/27094567 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Biochemistry and cell biology Engineering Materials engineering Atomic-scale analysis Biological interfaces Ferritin protein Atom probe tomography Inorganic-organic interface Nanomaterials Health and energy applications |
| dc.title.none.fl_str_mv | Atom Probe Tomographic Mapping Directly Reveals the Atomic Distribution of Phosphorus in Resin Embedded Ferritin |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Here we report the atomic-scale analysis of biological interfaces within the ferritin protein using atom probe tomography that is facilitated by an advanced specimen preparation approach. Embedding ferritin in an organic polymer resin lacking nitrogen provided chemical contrast to visualise atomic distributions and distinguish the inorganic-organic interface of the ferrihydrite mineral core and protein shell, as well as the organic-organic interface between the ferritin protein shell and embedding resin. In addition, we definitively show the atomic-scale distribution of phosphorus as being at the surface of the ferrihydrite mineral with the distribution of sodium mapped within the protein shell environment with an enhanced distribution at the mineral/protein interface. The sample preparation method is robust and can be directly extended to further enhance the study of biological, organic and inorganic nanomaterials relevant to health, energy or the environment.</p><h2>Other Information</h2><p dir="ltr">Published in: Scientific Reports<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1038/srep22321" target="_blank">https://dx.doi.org/10.1038/srep22321</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_dfaaaca1465aa26e4ea22a230b192b95 |
| identifier_str_mv | 10.1038/srep22321 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/27094567 |
| publishDate | 2016 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Atom Probe Tomographic Mapping Directly Reveals the Atomic Distribution of Phosphorus in Resin Embedded FerritinDaniel E. Perea (1767190)Jia Liu (41593)Jonah Bartrand (19730746)Quinten Dicken (19730749)S. Theva Thevuthasan (19730752)Nigel D. Browning (1234674)James E. Evans (7485)Biological sciencesBiochemistry and cell biologyEngineeringMaterials engineeringAtomic-scale analysisBiological interfacesFerritin proteinAtom probe tomographyInorganic-organic interfaceNanomaterialsHealth and energy applications<p dir="ltr">Here we report the atomic-scale analysis of biological interfaces within the ferritin protein using atom probe tomography that is facilitated by an advanced specimen preparation approach. Embedding ferritin in an organic polymer resin lacking nitrogen provided chemical contrast to visualise atomic distributions and distinguish the inorganic-organic interface of the ferrihydrite mineral core and protein shell, as well as the organic-organic interface between the ferritin protein shell and embedding resin. In addition, we definitively show the atomic-scale distribution of phosphorus as being at the surface of the ferrihydrite mineral with the distribution of sodium mapped within the protein shell environment with an enhanced distribution at the mineral/protein interface. The sample preparation method is robust and can be directly extended to further enhance the study of biological, organic and inorganic nanomaterials relevant to health, energy or the environment.</p><h2>Other Information</h2><p dir="ltr">Published in: Scientific Reports<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1038/srep22321" target="_blank">https://dx.doi.org/10.1038/srep22321</a></p>2016-02-29T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1038/srep22321https://figshare.com/articles/journal_contribution/Atom_Probe_Tomographic_Mapping_Directly_Reveals_the_Atomic_Distribution_of_Phosphorus_in_Resin_Embedded_Ferritin/27094567CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/270945672016-02-29T03:00:00Z |
| spellingShingle | Atom Probe Tomographic Mapping Directly Reveals the Atomic Distribution of Phosphorus in Resin Embedded Ferritin Daniel E. Perea (1767190) Biological sciences Biochemistry and cell biology Engineering Materials engineering Atomic-scale analysis Biological interfaces Ferritin protein Atom probe tomography Inorganic-organic interface Nanomaterials Health and energy applications |
| status_str | publishedVersion |
| title | Atom Probe Tomographic Mapping Directly Reveals the Atomic Distribution of Phosphorus in Resin Embedded Ferritin |
| title_full | Atom Probe Tomographic Mapping Directly Reveals the Atomic Distribution of Phosphorus in Resin Embedded Ferritin |
| title_fullStr | Atom Probe Tomographic Mapping Directly Reveals the Atomic Distribution of Phosphorus in Resin Embedded Ferritin |
| title_full_unstemmed | Atom Probe Tomographic Mapping Directly Reveals the Atomic Distribution of Phosphorus in Resin Embedded Ferritin |
| title_short | Atom Probe Tomographic Mapping Directly Reveals the Atomic Distribution of Phosphorus in Resin Embedded Ferritin |
| title_sort | Atom Probe Tomographic Mapping Directly Reveals the Atomic Distribution of Phosphorus in Resin Embedded Ferritin |
| topic | Biological sciences Biochemistry and cell biology Engineering Materials engineering Atomic-scale analysis Biological interfaces Ferritin protein Atom probe tomography Inorganic-organic interface Nanomaterials Health and energy applications |