Scalable Generation of Mesenchymal Stem Cells and Adipocytes from Human Pluripotent Stem Cells
<p dir="ltr">Human pluripotent stem cells (hPSCs) can provide unlimited supply for mesenchymal stem cells (MSCs) and adipocytes that can be used for therapeutic applications. Here we developed a simple and highly efficient all-trans-retinoic acid (RA)-based method for generating an o...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , |
| منشور في: |
2020
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| الموضوعات: | |
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| _version_ | 1864513515782406144 |
|---|---|
| author | Manale Karam (3619412) |
| author2 | Ihab Younis (57824) Noor R. Elareer (18583060) Sara Nasser (318373) Essam M. Abdelalim (5768072) |
| author2_role | author author author author |
| author_facet | Manale Karam (3619412) Ihab Younis (57824) Noor R. Elareer (18583060) Sara Nasser (318373) Essam M. Abdelalim (5768072) |
| author_role | author |
| dc.creator.none.fl_str_mv | Manale Karam (3619412) Ihab Younis (57824) Noor R. Elareer (18583060) Sara Nasser (318373) Essam M. Abdelalim (5768072) |
| dc.date.none.fl_str_mv | 2020-03-13T09:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3390/cells9030710 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Scalable_Generation_of_Mesenchymal_Stem_Cells_and_Adipocytes_from_Human_Pluripotent_Stem_Cells/25858678 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Medical biochemistry and metabolomics retinoic acid hPSCs MSCs apoptosis proliferation RNA-Seq Adipogenic differentiation |
| dc.title.none.fl_str_mv | Scalable Generation of Mesenchymal Stem Cells and Adipocytes from Human Pluripotent Stem Cells |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Human pluripotent stem cells (hPSCs) can provide unlimited supply for mesenchymal stem cells (MSCs) and adipocytes that can be used for therapeutic applications. Here we developed a simple and highly efficient all-trans-retinoic acid (RA)-based method for generating an off-the-shelf and scalable number of human pluripotent stem cell (hPSC)-derived MSCs with enhanced adipogenic potential. We showed that short exposure of multiple hPSC lines (hESCs/hiPSCs) to 10 μM RA dramatically enhances embryoid body (EB) formation through regulation of genes activating signaling pathways associated with cell proliferation, survival and adhesion, among others. Disruption of cell adhesion induced the subsequent differentiation of the highly expanded RA-derived EB-forming cells into a pure population of multipotent MSCs (up to 1542-fold increase in comparison to RA-untreated counterparts). Interestingly, the RA-derived MSCs displayed enhanced differentiation potential into adipocytes. Thus, these findings present a novel RA-based approach for providing an unlimited source of MSCs and adipocytes that can be used for regenerative medicine, drug screening and disease modeling applications.</p><h2>Other Information</h2><p dir="ltr">Published in: Cells<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/cells9030710" target="_blank">https://dx.doi.org/10.3390/cells9030710</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_dfb5ea099aef19c45d768ccd6d22169b |
| identifier_str_mv | 10.3390/cells9030710 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25858678 |
| publishDate | 2020 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Scalable Generation of Mesenchymal Stem Cells and Adipocytes from Human Pluripotent Stem CellsManale Karam (3619412)Ihab Younis (57824)Noor R. Elareer (18583060)Sara Nasser (318373)Essam M. Abdelalim (5768072)Biological sciencesBiochemistry and cell biologyBiomedical and clinical sciencesMedical biochemistry and metabolomicsretinoic acidhPSCsMSCsapoptosisproliferationRNA-SeqAdipogenic differentiation<p dir="ltr">Human pluripotent stem cells (hPSCs) can provide unlimited supply for mesenchymal stem cells (MSCs) and adipocytes that can be used for therapeutic applications. Here we developed a simple and highly efficient all-trans-retinoic acid (RA)-based method for generating an off-the-shelf and scalable number of human pluripotent stem cell (hPSC)-derived MSCs with enhanced adipogenic potential. We showed that short exposure of multiple hPSC lines (hESCs/hiPSCs) to 10 μM RA dramatically enhances embryoid body (EB) formation through regulation of genes activating signaling pathways associated with cell proliferation, survival and adhesion, among others. Disruption of cell adhesion induced the subsequent differentiation of the highly expanded RA-derived EB-forming cells into a pure population of multipotent MSCs (up to 1542-fold increase in comparison to RA-untreated counterparts). Interestingly, the RA-derived MSCs displayed enhanced differentiation potential into adipocytes. Thus, these findings present a novel RA-based approach for providing an unlimited source of MSCs and adipocytes that can be used for regenerative medicine, drug screening and disease modeling applications.</p><h2>Other Information</h2><p dir="ltr">Published in: Cells<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/cells9030710" target="_blank">https://dx.doi.org/10.3390/cells9030710</a></p>2020-03-13T09:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3390/cells9030710https://figshare.com/articles/journal_contribution/Scalable_Generation_of_Mesenchymal_Stem_Cells_and_Adipocytes_from_Human_Pluripotent_Stem_Cells/25858678CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/258586782020-03-13T09:00:00Z |
| spellingShingle | Scalable Generation of Mesenchymal Stem Cells and Adipocytes from Human Pluripotent Stem Cells Manale Karam (3619412) Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Medical biochemistry and metabolomics retinoic acid hPSCs MSCs apoptosis proliferation RNA-Seq Adipogenic differentiation |
| status_str | publishedVersion |
| title | Scalable Generation of Mesenchymal Stem Cells and Adipocytes from Human Pluripotent Stem Cells |
| title_full | Scalable Generation of Mesenchymal Stem Cells and Adipocytes from Human Pluripotent Stem Cells |
| title_fullStr | Scalable Generation of Mesenchymal Stem Cells and Adipocytes from Human Pluripotent Stem Cells |
| title_full_unstemmed | Scalable Generation of Mesenchymal Stem Cells and Adipocytes from Human Pluripotent Stem Cells |
| title_short | Scalable Generation of Mesenchymal Stem Cells and Adipocytes from Human Pluripotent Stem Cells |
| title_sort | Scalable Generation of Mesenchymal Stem Cells and Adipocytes from Human Pluripotent Stem Cells |
| topic | Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Medical biochemistry and metabolomics retinoic acid hPSCs MSCs apoptosis proliferation RNA-Seq Adipogenic differentiation |