Holocranohistochemistry enables the visualization of α-synuclein expression in the murine olfactory system and discovery of its systemic anti-microbial effects

<p dir="ltr">Braak and Del Tredici have proposed that typical Parkinson disease (PD) has its origins in the olfactory bulb and gastrointestinal tract. However, the role of the olfactory system has insufficiently been explored in the pathogeneses of PD and Alzheimer disease (AD) in la...

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Main Author: Julianna J. Tomlinson (15257035) (author)
Other Authors: Bojan Shutinoski (15257026) (author), Li Dong (37011) (author), Fanyi Meng (507361) (author), Dina Elleithy (19725451) (author), Nathalie A. Lengacher (15257011) (author), Angela P. Nguyen (14252939) (author), Greg O. Cron (9936684) (author), Qiubo Jiang (15257020) (author), Erik D. Roberson (7607492) (author), Robert L. Nussbaum (11287728) (author), Nour K. Majbour (8809316) (author), Omar M. El-Agnaf (3172641) (author), Steffany A. Bennett (19725454) (author), Diane C. Lagace (11973309) (author), John M. Woulfe (19725457) (author), Subash Sad (183253) (author), Earl G. Brown (19725460) (author), Michael G. Schlossmacher (3172692) (author)
Published: 2017
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author Julianna J. Tomlinson (15257035)
author2 Bojan Shutinoski (15257026)
Li Dong (37011)
Fanyi Meng (507361)
Dina Elleithy (19725451)
Nathalie A. Lengacher (15257011)
Angela P. Nguyen (14252939)
Greg O. Cron (9936684)
Qiubo Jiang (15257020)
Erik D. Roberson (7607492)
Robert L. Nussbaum (11287728)
Nour K. Majbour (8809316)
Omar M. El-Agnaf (3172641)
Steffany A. Bennett (19725454)
Diane C. Lagace (11973309)
John M. Woulfe (19725457)
Subash Sad (183253)
Earl G. Brown (19725460)
Michael G. Schlossmacher (3172692)
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author_facet Julianna J. Tomlinson (15257035)
Bojan Shutinoski (15257026)
Li Dong (37011)
Fanyi Meng (507361)
Dina Elleithy (19725451)
Nathalie A. Lengacher (15257011)
Angela P. Nguyen (14252939)
Greg O. Cron (9936684)
Qiubo Jiang (15257020)
Erik D. Roberson (7607492)
Robert L. Nussbaum (11287728)
Nour K. Majbour (8809316)
Omar M. El-Agnaf (3172641)
Steffany A. Bennett (19725454)
Diane C. Lagace (11973309)
John M. Woulfe (19725457)
Subash Sad (183253)
Earl G. Brown (19725460)
Michael G. Schlossmacher (3172692)
author_role author
dc.creator.none.fl_str_mv Julianna J. Tomlinson (15257035)
Bojan Shutinoski (15257026)
Li Dong (37011)
Fanyi Meng (507361)
Dina Elleithy (19725451)
Nathalie A. Lengacher (15257011)
Angela P. Nguyen (14252939)
Greg O. Cron (9936684)
Qiubo Jiang (15257020)
Erik D. Roberson (7607492)
Robert L. Nussbaum (11287728)
Nour K. Majbour (8809316)
Omar M. El-Agnaf (3172641)
Steffany A. Bennett (19725454)
Diane C. Lagace (11973309)
John M. Woulfe (19725457)
Subash Sad (183253)
Earl G. Brown (19725460)
Michael G. Schlossmacher (3172692)
dc.date.none.fl_str_mv 2017-05-05T03:00:00Z
dc.identifier.none.fl_str_mv 10.1007/s00702-017-1726-7
dc.relation.none.fl_str_mv https://figshare.com/articles/journal_contribution/Holocranohistochemistry_enables_the_visualization_of_-synuclein_expression_in_the_murine_olfactory_system_and_discovery_of_its_systemic_anti-microbial_effects/27088042
dc.rights.none.fl_str_mv CC BY 4.0
info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Biomedical and clinical sciences
Neurosciences
Histology
Parkinson disease
Alzheimer disease
Synucleinopathy
Neuropathology
SNCA/α-synuclein
MAPT/tau
APP/Aβ
Genome
Susceptibility
Exposome
Inoculation
Infection
dc.title.none.fl_str_mv Holocranohistochemistry enables the visualization of α-synuclein expression in the murine olfactory system and discovery of its systemic anti-microbial effects
dc.type.none.fl_str_mv Text
Journal contribution
info:eu-repo/semantics/publishedVersion
text
contribution to journal
description <p dir="ltr">Braak and Del Tredici have proposed that typical Parkinson disease (PD) has its origins in the olfactory bulb and gastrointestinal tract. However, the role of the olfactory system has insufficiently been explored in the pathogeneses of PD and Alzheimer disease (AD) in laboratory models. Here, we demonstrate applications of a new method to process mouse heads for microscopy by sectioning, mounting, and staining whole skulls (‘holocranohistochemistry’). This technique permits the visualization of the olfactory system from the nasal cavity to mitral cells and dopamine-producing interneurons of glomeruli in the olfactory bulb. We applied this method to two specific goals: first, to visualize PD- and AD-linked</p><h2>Other Information</h2><p dir="ltr">Published in: Journal of Neural Transmission<br>License: <a href="http://creativecommons.org/licenses/by/4.0" target="_blank">http://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1007/s00702-017-1726-7" target="_blank">https://dx.doi.org/10.1007/s00702-017-1726-7</a></p>
eu_rights_str_mv openAccess
id Manara2_e12493487ce3812041e06957a48b4e21
identifier_str_mv 10.1007/s00702-017-1726-7
network_acronym_str Manara2
network_name_str Manara2
oai_identifier_str oai:figshare.com:article/27088042
publishDate 2017
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spelling Holocranohistochemistry enables the visualization of α-synuclein expression in the murine olfactory system and discovery of its systemic anti-microbial effectsJulianna J. Tomlinson (15257035)Bojan Shutinoski (15257026)Li Dong (37011)Fanyi Meng (507361)Dina Elleithy (19725451)Nathalie A. Lengacher (15257011)Angela P. Nguyen (14252939)Greg O. Cron (9936684)Qiubo Jiang (15257020)Erik D. Roberson (7607492)Robert L. Nussbaum (11287728)Nour K. Majbour (8809316)Omar M. El-Agnaf (3172641)Steffany A. Bennett (19725454)Diane C. Lagace (11973309)John M. Woulfe (19725457)Subash Sad (183253)Earl G. Brown (19725460)Michael G. Schlossmacher (3172692)Biomedical and clinical sciencesNeurosciencesHistologyParkinson diseaseAlzheimer diseaseSynucleinopathyNeuropathologySNCA/α-synucleinMAPT/tauAPP/AβGenomeSusceptibilityExposomeInoculationInfection<p dir="ltr">Braak and Del Tredici have proposed that typical Parkinson disease (PD) has its origins in the olfactory bulb and gastrointestinal tract. However, the role of the olfactory system has insufficiently been explored in the pathogeneses of PD and Alzheimer disease (AD) in laboratory models. Here, we demonstrate applications of a new method to process mouse heads for microscopy by sectioning, mounting, and staining whole skulls (‘holocranohistochemistry’). This technique permits the visualization of the olfactory system from the nasal cavity to mitral cells and dopamine-producing interneurons of glomeruli in the olfactory bulb. We applied this method to two specific goals: first, to visualize PD- and AD-linked</p><h2>Other Information</h2><p dir="ltr">Published in: Journal of Neural Transmission<br>License: <a href="http://creativecommons.org/licenses/by/4.0" target="_blank">http://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1007/s00702-017-1726-7" target="_blank">https://dx.doi.org/10.1007/s00702-017-1726-7</a></p>2017-05-05T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1007/s00702-017-1726-7https://figshare.com/articles/journal_contribution/Holocranohistochemistry_enables_the_visualization_of_-synuclein_expression_in_the_murine_olfactory_system_and_discovery_of_its_systemic_anti-microbial_effects/27088042CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/270880422017-05-05T03:00:00Z
spellingShingle Holocranohistochemistry enables the visualization of α-synuclein expression in the murine olfactory system and discovery of its systemic anti-microbial effects
Julianna J. Tomlinson (15257035)
Biomedical and clinical sciences
Neurosciences
Histology
Parkinson disease
Alzheimer disease
Synucleinopathy
Neuropathology
SNCA/α-synuclein
MAPT/tau
APP/Aβ
Genome
Susceptibility
Exposome
Inoculation
Infection
status_str publishedVersion
title Holocranohistochemistry enables the visualization of α-synuclein expression in the murine olfactory system and discovery of its systemic anti-microbial effects
title_full Holocranohistochemistry enables the visualization of α-synuclein expression in the murine olfactory system and discovery of its systemic anti-microbial effects
title_fullStr Holocranohistochemistry enables the visualization of α-synuclein expression in the murine olfactory system and discovery of its systemic anti-microbial effects
title_full_unstemmed Holocranohistochemistry enables the visualization of α-synuclein expression in the murine olfactory system and discovery of its systemic anti-microbial effects
title_short Holocranohistochemistry enables the visualization of α-synuclein expression in the murine olfactory system and discovery of its systemic anti-microbial effects
title_sort Holocranohistochemistry enables the visualization of α-synuclein expression in the murine olfactory system and discovery of its systemic anti-microbial effects
topic Biomedical and clinical sciences
Neurosciences
Histology
Parkinson disease
Alzheimer disease
Synucleinopathy
Neuropathology
SNCA/α-synuclein
MAPT/tau
APP/Aβ
Genome
Susceptibility
Exposome
Inoculation
Infection