Airway surface liquid volume expansion induces rapid changes in amiloride-sensitive Na<sup>+</sup>transport across upper airway epithelium-Implications concerning the resolution of pulmonary edema
<p dir="ltr">During airway inflammation, airway surface liquid volume (ASLV) expansion may result from the movement of plasma proteins and excess liquid into the airway lumen due to extravasation and elevation of subepithelial hydrostatic pressure. We previously demonstrated that ele...
محفوظ في:
| المؤلف الرئيسي: | |
|---|---|
| مؤلفون آخرون: | , |
| منشور في: |
2015
|
| الموضوعات: | |
| الوسوم: |
إضافة وسم
لا توجد وسوم, كن أول من يضع وسما على هذه التسجيلة!
|
| _version_ | 1864513557236809728 |
|---|---|
| author | Fouad Azizi (11835843) |
| author2 | Abdelilah Arredouani (10914455) Ramzi M. Mohammad (14152737) |
| author2_role | author author |
| author_facet | Fouad Azizi (11835843) Abdelilah Arredouani (10914455) Ramzi M. Mohammad (14152737) |
| author_role | author |
| dc.creator.none.fl_str_mv | Fouad Azizi (11835843) Abdelilah Arredouani (10914455) Ramzi M. Mohammad (14152737) |
| dc.date.none.fl_str_mv | 2015-09-02T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.14814/phy2.12453 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Airway_surface_liquid_volume_expansion_induces_rapid_changes_in_amiloride-sensitive_Na_sup_sup_transport_across_upper_airway_epithelium-Implications_concerning_the_resolution_of_pulmonary_edema/27045052 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Clinical sciences Medical physiology Airway Inflammation Airway Surface Liquid Volume (ASLV) Hydrostatic Pressure Epithelial Permeability Sodium Transport Active Ion Transport Tracheal Epithelium |
| dc.title.none.fl_str_mv | Airway surface liquid volume expansion induces rapid changes in amiloride-sensitive Na<sup>+</sup>transport across upper airway epithelium-Implications concerning the resolution of pulmonary edema |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">During airway inflammation, airway surface liquid volume (ASLV) expansion may result from the movement of plasma proteins and excess liquid into the airway lumen due to extravasation and elevation of subepithelial hydrostatic pressure. We previously demonstrated that elevation of submucosal hydrostatic pressure increases airway epithelium permeability resulting in ASLV expansion by 500 μL cm<sup>−2</sup> h<sup>−1</sup>. Liquid reabsorption by healthy airway epithelium is regulated by active Na+ transport at a rate of 5 μL cm<sup>−2</sup> h<sup>−1</sup>. Thus, during inflammation the airway epithelium may be submerged by a large volume of luminal liquid. Here, we have investigated the mechanism by which ASLV expansion alters active epithelial Na+ transport, and we have characterized the time course of the change. We used primary cultures of tracheal airway epithelium maintained under air interface (basal ASLV, depth is 7 ± 0.5 μm). To mimic airway flooding, ASLV was expanded to a depth of 5 mm. On switching from basal to expanded ASLV conditions, short-circuit current (<i>I</i><sub><em>sc</em></sub>, a measure of total transepithelial active ion transport) declined by 90% with a half-time (t1/2) of 1 h. 24 h after the switch, there was no significant change in ATP concentration nor in the number of functional sodium pumps as revealed by [3H]-ouabain binding. However, amiloride-sensitive uptake of <sup>22</sup>Na+ was reduced by 70% upon ASLV expansion. This process is reversible since after returning cells back to air interface, <i>I</i><sub><em>sc</em></sub> recovered with a t1/2 of 5–10 h. These results may have important clinical implications concerning the development of Na+ channels activators and resolution of pulmonary edema.</p><h2>Other Information</h2><p dir="ltr">Published in: Physiological Reports<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.14814/phy2.12453" target="_blank">https://dx.doi.org/10.14814/phy2.12453</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_e1f243f436f1f9693df1222420d3d898 |
| identifier_str_mv | 10.14814/phy2.12453 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/27045052 |
| publishDate | 2015 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Airway surface liquid volume expansion induces rapid changes in amiloride-sensitive Na<sup>+</sup>transport across upper airway epithelium-Implications concerning the resolution of pulmonary edemaFouad Azizi (11835843)Abdelilah Arredouani (10914455)Ramzi M. Mohammad (14152737)Biomedical and clinical sciencesClinical sciencesMedical physiologyAirway InflammationAirway Surface Liquid Volume (ASLV)Hydrostatic PressureEpithelial PermeabilitySodium TransportActive Ion TransportTracheal Epithelium<p dir="ltr">During airway inflammation, airway surface liquid volume (ASLV) expansion may result from the movement of plasma proteins and excess liquid into the airway lumen due to extravasation and elevation of subepithelial hydrostatic pressure. We previously demonstrated that elevation of submucosal hydrostatic pressure increases airway epithelium permeability resulting in ASLV expansion by 500 μL cm<sup>−2</sup> h<sup>−1</sup>. Liquid reabsorption by healthy airway epithelium is regulated by active Na+ transport at a rate of 5 μL cm<sup>−2</sup> h<sup>−1</sup>. Thus, during inflammation the airway epithelium may be submerged by a large volume of luminal liquid. Here, we have investigated the mechanism by which ASLV expansion alters active epithelial Na+ transport, and we have characterized the time course of the change. We used primary cultures of tracheal airway epithelium maintained under air interface (basal ASLV, depth is 7 ± 0.5 μm). To mimic airway flooding, ASLV was expanded to a depth of 5 mm. On switching from basal to expanded ASLV conditions, short-circuit current (<i>I</i><sub><em>sc</em></sub>, a measure of total transepithelial active ion transport) declined by 90% with a half-time (t1/2) of 1 h. 24 h after the switch, there was no significant change in ATP concentration nor in the number of functional sodium pumps as revealed by [3H]-ouabain binding. However, amiloride-sensitive uptake of <sup>22</sup>Na+ was reduced by 70% upon ASLV expansion. This process is reversible since after returning cells back to air interface, <i>I</i><sub><em>sc</em></sub> recovered with a t1/2 of 5–10 h. These results may have important clinical implications concerning the development of Na+ channels activators and resolution of pulmonary edema.</p><h2>Other Information</h2><p dir="ltr">Published in: Physiological Reports<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.14814/phy2.12453" target="_blank">https://dx.doi.org/10.14814/phy2.12453</a></p>2015-09-02T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.14814/phy2.12453https://figshare.com/articles/journal_contribution/Airway_surface_liquid_volume_expansion_induces_rapid_changes_in_amiloride-sensitive_Na_sup_sup_transport_across_upper_airway_epithelium-Implications_concerning_the_resolution_of_pulmonary_edema/27045052CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/270450522015-09-02T03:00:00Z |
| spellingShingle | Airway surface liquid volume expansion induces rapid changes in amiloride-sensitive Na<sup>+</sup>transport across upper airway epithelium-Implications concerning the resolution of pulmonary edema Fouad Azizi (11835843) Biomedical and clinical sciences Clinical sciences Medical physiology Airway Inflammation Airway Surface Liquid Volume (ASLV) Hydrostatic Pressure Epithelial Permeability Sodium Transport Active Ion Transport Tracheal Epithelium |
| status_str | publishedVersion |
| title | Airway surface liquid volume expansion induces rapid changes in amiloride-sensitive Na<sup>+</sup>transport across upper airway epithelium-Implications concerning the resolution of pulmonary edema |
| title_full | Airway surface liquid volume expansion induces rapid changes in amiloride-sensitive Na<sup>+</sup>transport across upper airway epithelium-Implications concerning the resolution of pulmonary edema |
| title_fullStr | Airway surface liquid volume expansion induces rapid changes in amiloride-sensitive Na<sup>+</sup>transport across upper airway epithelium-Implications concerning the resolution of pulmonary edema |
| title_full_unstemmed | Airway surface liquid volume expansion induces rapid changes in amiloride-sensitive Na<sup>+</sup>transport across upper airway epithelium-Implications concerning the resolution of pulmonary edema |
| title_short | Airway surface liquid volume expansion induces rapid changes in amiloride-sensitive Na<sup>+</sup>transport across upper airway epithelium-Implications concerning the resolution of pulmonary edema |
| title_sort | Airway surface liquid volume expansion induces rapid changes in amiloride-sensitive Na<sup>+</sup>transport across upper airway epithelium-Implications concerning the resolution of pulmonary edema |
| topic | Biomedical and clinical sciences Clinical sciences Medical physiology Airway Inflammation Airway Surface Liquid Volume (ASLV) Hydrostatic Pressure Epithelial Permeability Sodium Transport Active Ion Transport Tracheal Epithelium |