Untargeted Metabolomics Profiling Reveals Perturbations in Arginine-NO Metabolism in Middle Eastern Patients with Coronary Heart Disease
<p dir="ltr">Coronary heart disease (CHD) is a major cause of death in Middle Eastern (ME) populations, with current studies of the metabolic fingerprints of CHD lacking in diversity. Identification of specific biomarkers to uncover potential mechanisms for developing predictive mode...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , , , , , , , |
| منشور في: |
2022
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إضافة وسم
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| _version_ | 1864513519229075456 |
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| author | Ehsan Ullah (2698921) |
| author2 | Ayman El-Menyar (440103) Khalid Kunji (828224) Reem Elsousy (18372435) Haira R. B. Mokhtar (18372438) Eiman Ahmad (18372441) Maryam Al-Nesf (5712296) Alka Beotra (14780368) Mohammed Al-Maadheed (12204886) Vidya Mohamed-Ali (12204895) Mohamad Saad (214545) Jassim Al Suwaidi (284932) |
| author2_role | author author author author author author author author author author author |
| author_facet | Ehsan Ullah (2698921) Ayman El-Menyar (440103) Khalid Kunji (828224) Reem Elsousy (18372435) Haira R. B. Mokhtar (18372438) Eiman Ahmad (18372441) Maryam Al-Nesf (5712296) Alka Beotra (14780368) Mohammed Al-Maadheed (12204886) Vidya Mohamed-Ali (12204895) Mohamad Saad (214545) Jassim Al Suwaidi (284932) |
| author_role | author |
| dc.creator.none.fl_str_mv | Ehsan Ullah (2698921) Ayman El-Menyar (440103) Khalid Kunji (828224) Reem Elsousy (18372435) Haira R. B. Mokhtar (18372438) Eiman Ahmad (18372441) Maryam Al-Nesf (5712296) Alka Beotra (14780368) Mohammed Al-Maadheed (12204886) Vidya Mohamed-Ali (12204895) Mohamad Saad (214545) Jassim Al Suwaidi (284932) |
| dc.date.none.fl_str_mv | 2022-06-03T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3390/metabo12060517 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Untargeted_Metabolomics_Profiling_Reveals_Perturbations_in_Arginine-NO_Metabolism_in_Middle_Eastern_Patients_with_Coronary_Heart_Disease/25609449 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Cardiovascular medicine and haematology Medical biochemistry and metabolomics metabolomics coronary heart disease arginine metabolism metabolite risk score Middle East |
| dc.title.none.fl_str_mv | Untargeted Metabolomics Profiling Reveals Perturbations in Arginine-NO Metabolism in Middle Eastern Patients with Coronary Heart Disease |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Coronary heart disease (CHD) is a major cause of death in Middle Eastern (ME) populations, with current studies of the metabolic fingerprints of CHD lacking in diversity. Identification of specific biomarkers to uncover potential mechanisms for developing predictive models and targeted therapies for CHD is urgently needed for the least-studied ME populations. A case-control study was carried out in a cohort of 1001 CHD patients and 2999 controls. Untargeted metabolomics was used, generating 1159 metabolites. Univariate and pathway enrichment analyses were performed to understand functional changes in CHD. A metabolite risk score (MRS) was developed to assess the predictive performance of CHD using multivariate analysis and machine learning. A total of 511 metabolites were significantly different between the CHD patients and the controls (FDR p < 0.05). The enriched pathways (FDR p < 10−300) included D-arginine and D-ornithine metabolism, glycolysis, oxidation and degradation of branched chain fatty acids, and sphingolipid metabolism. MRS showed good discriminative power between the CHD cases and the controls (AUC = 0.99). In this first study in the Middle East, known and novel circulating metabolites and metabolic pathways associated with CHD were identified. A small panel of metabolites can efficiently discriminate CHD cases and controls and therefore can be used as a diagnostic/predictive tool.</p><h2>Other Information</h2><p dir="ltr">Published in: Metabolites<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/metabo12060517" target="_blank">https://dx.doi.org/10.3390/metabo12060517</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_e2bf70c7d1de32bae714347840da8512 |
| identifier_str_mv | 10.3390/metabo12060517 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25609449 |
| publishDate | 2022 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Untargeted Metabolomics Profiling Reveals Perturbations in Arginine-NO Metabolism in Middle Eastern Patients with Coronary Heart DiseaseEhsan Ullah (2698921)Ayman El-Menyar (440103)Khalid Kunji (828224)Reem Elsousy (18372435)Haira R. B. Mokhtar (18372438)Eiman Ahmad (18372441)Maryam Al-Nesf (5712296)Alka Beotra (14780368)Mohammed Al-Maadheed (12204886)Vidya Mohamed-Ali (12204895)Mohamad Saad (214545)Jassim Al Suwaidi (284932)Biomedical and clinical sciencesCardiovascular medicine and haematologyMedical biochemistry and metabolomicsmetabolomicscoronary heart diseasearginine metabolismmetabolite risk scoreMiddle East<p dir="ltr">Coronary heart disease (CHD) is a major cause of death in Middle Eastern (ME) populations, with current studies of the metabolic fingerprints of CHD lacking in diversity. Identification of specific biomarkers to uncover potential mechanisms for developing predictive models and targeted therapies for CHD is urgently needed for the least-studied ME populations. A case-control study was carried out in a cohort of 1001 CHD patients and 2999 controls. Untargeted metabolomics was used, generating 1159 metabolites. Univariate and pathway enrichment analyses were performed to understand functional changes in CHD. A metabolite risk score (MRS) was developed to assess the predictive performance of CHD using multivariate analysis and machine learning. A total of 511 metabolites were significantly different between the CHD patients and the controls (FDR p < 0.05). The enriched pathways (FDR p < 10−300) included D-arginine and D-ornithine metabolism, glycolysis, oxidation and degradation of branched chain fatty acids, and sphingolipid metabolism. MRS showed good discriminative power between the CHD cases and the controls (AUC = 0.99). In this first study in the Middle East, known and novel circulating metabolites and metabolic pathways associated with CHD were identified. A small panel of metabolites can efficiently discriminate CHD cases and controls and therefore can be used as a diagnostic/predictive tool.</p><h2>Other Information</h2><p dir="ltr">Published in: Metabolites<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/metabo12060517" target="_blank">https://dx.doi.org/10.3390/metabo12060517</a></p>2022-06-03T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3390/metabo12060517https://figshare.com/articles/journal_contribution/Untargeted_Metabolomics_Profiling_Reveals_Perturbations_in_Arginine-NO_Metabolism_in_Middle_Eastern_Patients_with_Coronary_Heart_Disease/25609449CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/256094492022-06-03T03:00:00Z |
| spellingShingle | Untargeted Metabolomics Profiling Reveals Perturbations in Arginine-NO Metabolism in Middle Eastern Patients with Coronary Heart Disease Ehsan Ullah (2698921) Biomedical and clinical sciences Cardiovascular medicine and haematology Medical biochemistry and metabolomics metabolomics coronary heart disease arginine metabolism metabolite risk score Middle East |
| status_str | publishedVersion |
| title | Untargeted Metabolomics Profiling Reveals Perturbations in Arginine-NO Metabolism in Middle Eastern Patients with Coronary Heart Disease |
| title_full | Untargeted Metabolomics Profiling Reveals Perturbations in Arginine-NO Metabolism in Middle Eastern Patients with Coronary Heart Disease |
| title_fullStr | Untargeted Metabolomics Profiling Reveals Perturbations in Arginine-NO Metabolism in Middle Eastern Patients with Coronary Heart Disease |
| title_full_unstemmed | Untargeted Metabolomics Profiling Reveals Perturbations in Arginine-NO Metabolism in Middle Eastern Patients with Coronary Heart Disease |
| title_short | Untargeted Metabolomics Profiling Reveals Perturbations in Arginine-NO Metabolism in Middle Eastern Patients with Coronary Heart Disease |
| title_sort | Untargeted Metabolomics Profiling Reveals Perturbations in Arginine-NO Metabolism in Middle Eastern Patients with Coronary Heart Disease |
| topic | Biomedical and clinical sciences Cardiovascular medicine and haematology Medical biochemistry and metabolomics metabolomics coronary heart disease arginine metabolism metabolite risk score Middle East |