Identification of potential transcription factors that enhance human iPSC generation
<div><p>Although many factors have been identified and used to enhance the iPSC reprogramming process, its efficiency remains quite low. In addition, reprogramming efficacy has been evidenced to be affected by disease mutations that are present in patient samples. In this study, using RN...
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| مؤلفون آخرون: | , , , , , , , |
| منشور في: |
2020
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| _version_ | 1864513512965931008 |
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| author | Nuha T. Swaidan (18595219) |
| author2 | Salam Salloum-Asfar (656363) Freshteh Palangi (4278685) Khaoula Errafii (10914446) Nada H. Soliman (18595222) Ahmed T. Aboughalia (18595225) Abdul Haseeb S. Wali (18595228) Sara A. Abdulla (13902015) Mohamed M. Emara (9913215) |
| author2_role | author author author author author author author author |
| author_facet | Nuha T. Swaidan (18595219) Salam Salloum-Asfar (656363) Freshteh Palangi (4278685) Khaoula Errafii (10914446) Nada H. Soliman (18595222) Ahmed T. Aboughalia (18595225) Abdul Haseeb S. Wali (18595228) Sara A. Abdulla (13902015) Mohamed M. Emara (9913215) |
| author_role | author |
| dc.creator.none.fl_str_mv | Nuha T. Swaidan (18595219) Salam Salloum-Asfar (656363) Freshteh Palangi (4278685) Khaoula Errafii (10914446) Nada H. Soliman (18595222) Ahmed T. Aboughalia (18595225) Abdul Haseeb S. Wali (18595228) Sara A. Abdulla (13902015) Mohamed M. Emara (9913215) |
| dc.date.none.fl_str_mv | 2020-12-15T06:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1038/s41598-020-78932-9 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Identification_of_potential_transcription_factors_that_enhance_human_iPSC_generation/25953916 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Genetics Biomedical and clinical sciences Medical biotechnology Neurosciences Molecular biology Stem cells Somatic cell reprogramming Pluripotency genes Parkinson’s disease |
| dc.title.none.fl_str_mv | Identification of potential transcription factors that enhance human iPSC generation |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <div><p>Although many factors have been identified and used to enhance the iPSC reprogramming process, its efficiency remains quite low. In addition, reprogramming efficacy has been evidenced to be affected by disease mutations that are present in patient samples. In this study, using RNA-seq platform we have identified and validated the differential gene expression of five transcription factors (TFs) (GBX2, NANOGP8, SP8, PEG3, and ZIC1) that were associated with a remarkable increase in the number of iPSC colonies generated from a patient with Parkinson's disease. We have applied different bioinformatics tools (Gene ontology, protein–protein interaction, and signaling pathways analyses) to investigate the possible roles of these TFs in pluripotency and developmental process. Interestingly, GBX2, NANOGP8, SP8, PEG3, and ZIC1 were found to play a role in maintaining pluripotency, regulating self-renewal stages, and interacting with other factors that are involved in pluripotency regulation including OCT4, SOX2, NANOG, and KLF4. Therefore, the TFs identified in this study could be used as additional transcription factors that enhance reprogramming efficiency to boost iPSC generation technology.</p><p> </p></div><h2>Other Information</h2> <p> Published in: Scientific Reports<br> License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1038/s41598-020-78932-9" target="_blank">https://dx.doi.org/10.1038/s41598-020-78932-9</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_e5627b647b8489fd865b4c8c9d8f56f2 |
| identifier_str_mv | 10.1038/s41598-020-78932-9 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25953916 |
| publishDate | 2020 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Identification of potential transcription factors that enhance human iPSC generationNuha T. Swaidan (18595219)Salam Salloum-Asfar (656363)Freshteh Palangi (4278685)Khaoula Errafii (10914446)Nada H. Soliman (18595222)Ahmed T. Aboughalia (18595225)Abdul Haseeb S. Wali (18595228)Sara A. Abdulla (13902015)Mohamed M. Emara (9913215)Biological sciencesGeneticsBiomedical and clinical sciencesMedical biotechnologyNeurosciencesMolecular biologyStem cellsSomatic cell reprogrammingPluripotency genesParkinson’s disease<div><p>Although many factors have been identified and used to enhance the iPSC reprogramming process, its efficiency remains quite low. In addition, reprogramming efficacy has been evidenced to be affected by disease mutations that are present in patient samples. In this study, using RNA-seq platform we have identified and validated the differential gene expression of five transcription factors (TFs) (GBX2, NANOGP8, SP8, PEG3, and ZIC1) that were associated with a remarkable increase in the number of iPSC colonies generated from a patient with Parkinson's disease. We have applied different bioinformatics tools (Gene ontology, protein–protein interaction, and signaling pathways analyses) to investigate the possible roles of these TFs in pluripotency and developmental process. Interestingly, GBX2, NANOGP8, SP8, PEG3, and ZIC1 were found to play a role in maintaining pluripotency, regulating self-renewal stages, and interacting with other factors that are involved in pluripotency regulation including OCT4, SOX2, NANOG, and KLF4. Therefore, the TFs identified in this study could be used as additional transcription factors that enhance reprogramming efficiency to boost iPSC generation technology.</p><p> </p></div><h2>Other Information</h2> <p> Published in: Scientific Reports<br> License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1038/s41598-020-78932-9" target="_blank">https://dx.doi.org/10.1038/s41598-020-78932-9</a></p>2020-12-15T06:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1038/s41598-020-78932-9https://figshare.com/articles/journal_contribution/Identification_of_potential_transcription_factors_that_enhance_human_iPSC_generation/25953916CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/259539162020-12-15T06:00:00Z |
| spellingShingle | Identification of potential transcription factors that enhance human iPSC generation Nuha T. Swaidan (18595219) Biological sciences Genetics Biomedical and clinical sciences Medical biotechnology Neurosciences Molecular biology Stem cells Somatic cell reprogramming Pluripotency genes Parkinson’s disease |
| status_str | publishedVersion |
| title | Identification of potential transcription factors that enhance human iPSC generation |
| title_full | Identification of potential transcription factors that enhance human iPSC generation |
| title_fullStr | Identification of potential transcription factors that enhance human iPSC generation |
| title_full_unstemmed | Identification of potential transcription factors that enhance human iPSC generation |
| title_short | Identification of potential transcription factors that enhance human iPSC generation |
| title_sort | Identification of potential transcription factors that enhance human iPSC generation |
| topic | Biological sciences Genetics Biomedical and clinical sciences Medical biotechnology Neurosciences Molecular biology Stem cells Somatic cell reprogramming Pluripotency genes Parkinson’s disease |