Anti-angiogenic effect of nano-formulated water soluble kaempferol and combretastatin in an in vivo chick chorioallantoic membrane model and HUVEC cells
<p>The present study evaluated the efficacy of nano-formulated water-soluble kaempferol and combretastatin alone and combined against the native kaempferol and combretastatin on angiogenesis. The solvent evaporation method was used to synthesize the nano-formulated water-soluble kaempferol and...
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2023
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| _version_ | 1864513530414235648 |
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| author | Gowtham Kumar Subbaraj (17807615) |
| author2 | Tariq Masoodi (8117702) Santhosh Kumar Yasam (17807618) Kirubhanand Chandrashekar (17807621) Langeswaran Kulanthaivel (17807624) Noor Ahmad Shaik (7889981) Sheema Hashem (14150961) Ammira S. Alshabeeb Akil (17807627) Ajaz A. Bhat (12984701) |
| author2_role | author author author author author author author author |
| author_facet | Gowtham Kumar Subbaraj (17807615) Tariq Masoodi (8117702) Santhosh Kumar Yasam (17807618) Kirubhanand Chandrashekar (17807621) Langeswaran Kulanthaivel (17807624) Noor Ahmad Shaik (7889981) Sheema Hashem (14150961) Ammira S. Alshabeeb Akil (17807627) Ajaz A. Bhat (12984701) |
| author_role | author |
| dc.creator.none.fl_str_mv | Gowtham Kumar Subbaraj (17807615) Tariq Masoodi (8117702) Santhosh Kumar Yasam (17807618) Kirubhanand Chandrashekar (17807621) Langeswaran Kulanthaivel (17807624) Noor Ahmad Shaik (7889981) Sheema Hashem (14150961) Ammira S. Alshabeeb Akil (17807627) Ajaz A. Bhat (12984701) |
| dc.date.none.fl_str_mv | 2023-07-01T00:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1016/j.biopha.2023.114820 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Anti-angiogenic_effect_of_nano-formulated_water_soluble_kaempferol_and_combretastatin_in_an_in_vivo_chick_chorioallantoic_membrane_model_and_HUVEC_cells/25018265 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Cardiovascular medicine and haematology Oncology and carcinogenesis Pharmacology and pharmaceutical sciences Angiogenesis Chorioallantoic membrane Nano-formulation Kaempferol Combretastatin |
| dc.title.none.fl_str_mv | Anti-angiogenic effect of nano-formulated water soluble kaempferol and combretastatin in an in vivo chick chorioallantoic membrane model and HUVEC cells |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p>The present study evaluated the efficacy of nano-formulated water-soluble kaempferol and combretastatin alone and combined against the native kaempferol and combretastatin on angiogenesis. The solvent evaporation method was used to synthesize the nano-formulated water-soluble kaempferol and combretastatin and characterized using various analyses such as dynamic light scattering (DLS) and Fourier-transform infrared (FT-IR) spectroscopy.The anti-angiogenic activity of native, nano-formulated water-soluble kaempferol and combretastatin was investigated by cell viability on HUVEC and A498 cell lines, while chick chorioallantoic membrane (CAM) assay was utilized to assess morphometric and histopathological changes, and mRNA expressions of VEGF-A and FGF2 using qRT-PCR. MTT assay results revealed that the combination of nano-formulated water-soluble kaempferol and combretastatin significantly reduced the cell viability compared to control, individual treatments of native, nano-formulated water-soluble kaempferol, and combretastatin. Morphometric analysis of CAM showed that treatment with nano-formulated water-soluble kaempferol and combretastatin caused a substantial decrease in density, vessel network, branch points, and nets of CAM blood vessels. The histopathological results of CAM showed the irregular shape of blood vessels at the thin stratum of chronic endoderm, and blood capillaries were diminished compared to the control. In addition, the mRNA expression levels of VEGF-A and FGF2 were significantly decreased compared with native forms. Therefore, the findings of this study indicate that nano-formulated water-soluble combretastatin and kaempferol suppress angiogenesis by preventing the activation of endothelial cells and suppressing factors of angiogenesis. Moreover, a combination of nano-formulated water-soluble kaempferol and combretastatin worked much better than individual treatments.</p><h2>Other Information</h2> <p> Published in: Biomedicine & Pharmacotherapy<br> License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.biopha.2023.114820" target="_blank">https://dx.doi.org/10.1016/j.biopha.2023.114820</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_e754c018f1dc07fbfcf3e692bbf202a1 |
| identifier_str_mv | 10.1016/j.biopha.2023.114820 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25018265 |
| publishDate | 2023 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Anti-angiogenic effect of nano-formulated water soluble kaempferol and combretastatin in an in vivo chick chorioallantoic membrane model and HUVEC cellsGowtham Kumar Subbaraj (17807615)Tariq Masoodi (8117702)Santhosh Kumar Yasam (17807618)Kirubhanand Chandrashekar (17807621)Langeswaran Kulanthaivel (17807624)Noor Ahmad Shaik (7889981)Sheema Hashem (14150961)Ammira S. Alshabeeb Akil (17807627)Ajaz A. Bhat (12984701)Biological sciencesBiochemistry and cell biologyBiomedical and clinical sciencesCardiovascular medicine and haematologyOncology and carcinogenesisPharmacology and pharmaceutical sciencesAngiogenesisChorioallantoic membraneNano-formulationKaempferolCombretastatin<p>The present study evaluated the efficacy of nano-formulated water-soluble kaempferol and combretastatin alone and combined against the native kaempferol and combretastatin on angiogenesis. The solvent evaporation method was used to synthesize the nano-formulated water-soluble kaempferol and combretastatin and characterized using various analyses such as dynamic light scattering (DLS) and Fourier-transform infrared (FT-IR) spectroscopy.The anti-angiogenic activity of native, nano-formulated water-soluble kaempferol and combretastatin was investigated by cell viability on HUVEC and A498 cell lines, while chick chorioallantoic membrane (CAM) assay was utilized to assess morphometric and histopathological changes, and mRNA expressions of VEGF-A and FGF2 using qRT-PCR. MTT assay results revealed that the combination of nano-formulated water-soluble kaempferol and combretastatin significantly reduced the cell viability compared to control, individual treatments of native, nano-formulated water-soluble kaempferol, and combretastatin. Morphometric analysis of CAM showed that treatment with nano-formulated water-soluble kaempferol and combretastatin caused a substantial decrease in density, vessel network, branch points, and nets of CAM blood vessels. The histopathological results of CAM showed the irregular shape of blood vessels at the thin stratum of chronic endoderm, and blood capillaries were diminished compared to the control. In addition, the mRNA expression levels of VEGF-A and FGF2 were significantly decreased compared with native forms. Therefore, the findings of this study indicate that nano-formulated water-soluble combretastatin and kaempferol suppress angiogenesis by preventing the activation of endothelial cells and suppressing factors of angiogenesis. Moreover, a combination of nano-formulated water-soluble kaempferol and combretastatin worked much better than individual treatments.</p><h2>Other Information</h2> <p> Published in: Biomedicine & Pharmacotherapy<br> License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.biopha.2023.114820" target="_blank">https://dx.doi.org/10.1016/j.biopha.2023.114820</a></p>2023-07-01T00:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1016/j.biopha.2023.114820https://figshare.com/articles/journal_contribution/Anti-angiogenic_effect_of_nano-formulated_water_soluble_kaempferol_and_combretastatin_in_an_in_vivo_chick_chorioallantoic_membrane_model_and_HUVEC_cells/25018265CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/250182652023-07-01T00:00:00Z |
| spellingShingle | Anti-angiogenic effect of nano-formulated water soluble kaempferol and combretastatin in an in vivo chick chorioallantoic membrane model and HUVEC cells Gowtham Kumar Subbaraj (17807615) Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Cardiovascular medicine and haematology Oncology and carcinogenesis Pharmacology and pharmaceutical sciences Angiogenesis Chorioallantoic membrane Nano-formulation Kaempferol Combretastatin |
| status_str | publishedVersion |
| title | Anti-angiogenic effect of nano-formulated water soluble kaempferol and combretastatin in an in vivo chick chorioallantoic membrane model and HUVEC cells |
| title_full | Anti-angiogenic effect of nano-formulated water soluble kaempferol and combretastatin in an in vivo chick chorioallantoic membrane model and HUVEC cells |
| title_fullStr | Anti-angiogenic effect of nano-formulated water soluble kaempferol and combretastatin in an in vivo chick chorioallantoic membrane model and HUVEC cells |
| title_full_unstemmed | Anti-angiogenic effect of nano-formulated water soluble kaempferol and combretastatin in an in vivo chick chorioallantoic membrane model and HUVEC cells |
| title_short | Anti-angiogenic effect of nano-formulated water soluble kaempferol and combretastatin in an in vivo chick chorioallantoic membrane model and HUVEC cells |
| title_sort | Anti-angiogenic effect of nano-formulated water soluble kaempferol and combretastatin in an in vivo chick chorioallantoic membrane model and HUVEC cells |
| topic | Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Cardiovascular medicine and haematology Oncology and carcinogenesis Pharmacology and pharmaceutical sciences Angiogenesis Chorioallantoic membrane Nano-formulation Kaempferol Combretastatin |