Membrane progesterone receptor induces meiosis in Xenopus oocytes through endocytosis into signaling endosomes and interaction with APPL1 and Akt2
<p dir="ltr">The steroid hormone progesterone (P4) mediates many physiological processes through either nuclear receptors that modulate gene expression or membrane P4 receptors (mPRs) that mediate nongenomic signaling. mPR signaling remains poorly understood. Here we show that the to...
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| مؤلفون آخرون: | , , , , , , , |
| منشور في: |
2020
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| _version_ | 1864513513520627712 |
|---|---|
| author | Nancy Nader (175869) |
| author2 | Maya Dib (9592362) Rawad Hodeify (9592365) Raphael Courjaret (9592368) Asha Elmi (9592371) Ayat S. Hammad (9592374) Raja Dey (265341) Xin-Yun Huang (374284) Khaled Machaca (194372) |
| author2_role | author author author author author author author author |
| author_facet | Nancy Nader (175869) Maya Dib (9592362) Rawad Hodeify (9592365) Raphael Courjaret (9592368) Asha Elmi (9592371) Ayat S. Hammad (9592374) Raja Dey (265341) Xin-Yun Huang (374284) Khaled Machaca (194372) |
| author_role | author |
| dc.creator.none.fl_str_mv | Nancy Nader (175869) Maya Dib (9592362) Rawad Hodeify (9592365) Raphael Courjaret (9592368) Asha Elmi (9592371) Ayat S. Hammad (9592374) Raja Dey (265341) Xin-Yun Huang (374284) Khaled Machaca (194372) |
| dc.date.none.fl_str_mv | 2020-11-02T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pbio.3000901 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Membrane_progesterone_receptor_induces_meiosis_in_Xenopus_oocytes_through_endocytosis_into_signaling_endosomes_and_interaction_with_APPL1_and_Akt2/25912135 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Clinical sciences Steroid hormone Progesterone (P4) Nuclear receptors Membrane progesterone receptors (mPRs) Topology Meiosis Signaling endosome |
| dc.title.none.fl_str_mv | Membrane progesterone receptor induces meiosis in Xenopus oocytes through endocytosis into signaling endosomes and interaction with APPL1 and Akt2 |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">The steroid hormone progesterone (P4) mediates many physiological processes through either nuclear receptors that modulate gene expression or membrane P4 receptors (mPRs) that mediate nongenomic signaling. mPR signaling remains poorly understood. Here we show that the topology of mPRβ is similar to adiponectin receptors and opposite to that of G-protein-coupled receptors (GPCRs). UsingXenopusoocyte meiosis as a well-established physiological readout of nongenomic P4 signaling, we demonstrate that mPRβ signaling requires the adaptor protein APPL1 and the kinase Akt2. We further show that P4 induces clathrin-dependent endocytosis of mPRβ into signaling endosome, where mPR interacts transiently with APPL1 and Akt2 to induce meiosis. Our findings outline the early steps involved in mPR signaling and expand the spectrum of mPR signaling through the multitude of pathways involving APPL1.<br><br>Correction: Correction: Membrane progesterone receptor induces meiosis in Xenopus oocytes through endocytosis into signaling endosomes and interaction with APPL1 and Akt2: <a href="https://doi.org/10.1371/journal.pbio.3001117" target="_blank">https://doi.org/10.1371/journal.pbio.3001117</a>, published online 10 February 2021.</p><h2>Other Information</h2><p dir="ltr">Published in: PLOS Biology<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1371/journal.pbio.3000901" target="_blank">https://dx.doi.org/10.1371/journal.pbio.3000901</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_e786192034d1b9fc9395e38863d88bb0 |
| identifier_str_mv | 10.1371/journal.pbio.3000901 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25912135 |
| publishDate | 2020 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Membrane progesterone receptor induces meiosis in Xenopus oocytes through endocytosis into signaling endosomes and interaction with APPL1 and Akt2Nancy Nader (175869)Maya Dib (9592362)Rawad Hodeify (9592365)Raphael Courjaret (9592368)Asha Elmi (9592371)Ayat S. Hammad (9592374)Raja Dey (265341)Xin-Yun Huang (374284)Khaled Machaca (194372)Biological sciencesBiochemistry and cell biologyBiomedical and clinical sciencesClinical sciencesSteroid hormoneProgesterone (P4)Nuclear receptorsMembrane progesterone receptors (mPRs)TopologyMeiosisSignaling endosome<p dir="ltr">The steroid hormone progesterone (P4) mediates many physiological processes through either nuclear receptors that modulate gene expression or membrane P4 receptors (mPRs) that mediate nongenomic signaling. mPR signaling remains poorly understood. Here we show that the topology of mPRβ is similar to adiponectin receptors and opposite to that of G-protein-coupled receptors (GPCRs). UsingXenopusoocyte meiosis as a well-established physiological readout of nongenomic P4 signaling, we demonstrate that mPRβ signaling requires the adaptor protein APPL1 and the kinase Akt2. We further show that P4 induces clathrin-dependent endocytosis of mPRβ into signaling endosome, where mPR interacts transiently with APPL1 and Akt2 to induce meiosis. Our findings outline the early steps involved in mPR signaling and expand the spectrum of mPR signaling through the multitude of pathways involving APPL1.<br><br>Correction: Correction: Membrane progesterone receptor induces meiosis in Xenopus oocytes through endocytosis into signaling endosomes and interaction with APPL1 and Akt2: <a href="https://doi.org/10.1371/journal.pbio.3001117" target="_blank">https://doi.org/10.1371/journal.pbio.3001117</a>, published online 10 February 2021.</p><h2>Other Information</h2><p dir="ltr">Published in: PLOS Biology<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1371/journal.pbio.3000901" target="_blank">https://dx.doi.org/10.1371/journal.pbio.3000901</a></p>2020-11-02T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1371/journal.pbio.3000901https://figshare.com/articles/journal_contribution/Membrane_progesterone_receptor_induces_meiosis_in_Xenopus_oocytes_through_endocytosis_into_signaling_endosomes_and_interaction_with_APPL1_and_Akt2/25912135CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/259121352020-11-02T03:00:00Z |
| spellingShingle | Membrane progesterone receptor induces meiosis in Xenopus oocytes through endocytosis into signaling endosomes and interaction with APPL1 and Akt2 Nancy Nader (175869) Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Clinical sciences Steroid hormone Progesterone (P4) Nuclear receptors Membrane progesterone receptors (mPRs) Topology Meiosis Signaling endosome |
| status_str | publishedVersion |
| title | Membrane progesterone receptor induces meiosis in Xenopus oocytes through endocytosis into signaling endosomes and interaction with APPL1 and Akt2 |
| title_full | Membrane progesterone receptor induces meiosis in Xenopus oocytes through endocytosis into signaling endosomes and interaction with APPL1 and Akt2 |
| title_fullStr | Membrane progesterone receptor induces meiosis in Xenopus oocytes through endocytosis into signaling endosomes and interaction with APPL1 and Akt2 |
| title_full_unstemmed | Membrane progesterone receptor induces meiosis in Xenopus oocytes through endocytosis into signaling endosomes and interaction with APPL1 and Akt2 |
| title_short | Membrane progesterone receptor induces meiosis in Xenopus oocytes through endocytosis into signaling endosomes and interaction with APPL1 and Akt2 |
| title_sort | Membrane progesterone receptor induces meiosis in Xenopus oocytes through endocytosis into signaling endosomes and interaction with APPL1 and Akt2 |
| topic | Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Clinical sciences Steroid hormone Progesterone (P4) Nuclear receptors Membrane progesterone receptors (mPRs) Topology Meiosis Signaling endosome |