Design of a 3D printed, motorized, uniaxial cell stretcher for microscopic and biochemical analysis of mechanotransduction
<div><p>Cells respond to mechanical cues from their environment through a process of mechanosensing and mechanotransduction. Cell stretching devices are important tools to study the molecular pathways responsible for cellular responses to mechanobiological processes. We describe the deve...
محفوظ في:
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| مؤلفون آخرون: | , |
| منشور في: |
2021
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إضافة وسم
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| _version_ | 1864513516708298752 |
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| author | Noor A. Al-Maslamani (17316925) |
| author2 | Abdulghani A. Khilan (17316922) Henning F. Horn (14152938) |
| author2_role | author author |
| author_facet | Noor A. Al-Maslamani (17316925) Abdulghani A. Khilan (17316922) Henning F. Horn (14152938) |
| author_role | author |
| dc.creator.none.fl_str_mv | Noor A. Al-Maslamani (17316925) Abdulghani A. Khilan (17316922) Henning F. Horn (14152938) |
| dc.date.none.fl_str_mv | 2021-02-10T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1242/bio.057778 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Design_of_a_3D_printed_motorized_uniaxial_cell_stretcher_for_microscopic_and_biochemical_analysis_of_mechanotransduction/25764876 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Biochemistry and cell biology Uniaxial stretcher Mechanobiology 3D printing Cell stretching |
| dc.title.none.fl_str_mv | Design of a 3D printed, motorized, uniaxial cell stretcher for microscopic and biochemical analysis of mechanotransduction |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <div><p>Cells respond to mechanical cues from their environment through a process of mechanosensing and mechanotransduction. Cell stretching devices are important tools to study the molecular pathways responsible for cellular responses to mechanobiological processes. We describe the development and testing of a uniaxial cell stretcher that has applications for microscopic as well as biochemical analyses. By combining simple fabrication techniques with adjustable control parameters, the stretcher is designed to fit a variety of experimental needs. The stretcher can be used for static and cyclic stretching. As a proof of principle, we visualize stretch induced deformation of cell nuclei via incremental static stretch, and changes in IEX1 expression via cyclic stretching. This stretcher is easily modified to meet experimental needs, inexpensive to build, and should be readily accessible for most laboratories with access to 3D printing.</p><p> </p></div><h2>Other Information</h2> <p> Published in: Biology Open<br> License: <a href="http://creativecommons.org/licenses/by/4.0" target="_blank">http://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1242/bio.057778" target="_blank">https://dx.doi.org/10.1242/bio.057778</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_e82a836c819966bbf867c461ea615c71 |
| identifier_str_mv | 10.1242/bio.057778 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25764876 |
| publishDate | 2021 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Design of a 3D printed, motorized, uniaxial cell stretcher for microscopic and biochemical analysis of mechanotransductionNoor A. Al-Maslamani (17316925)Abdulghani A. Khilan (17316922)Henning F. Horn (14152938)Biological sciencesBiochemistry and cell biologyUniaxial stretcherMechanobiology3D printingCell stretching<div><p>Cells respond to mechanical cues from their environment through a process of mechanosensing and mechanotransduction. Cell stretching devices are important tools to study the molecular pathways responsible for cellular responses to mechanobiological processes. We describe the development and testing of a uniaxial cell stretcher that has applications for microscopic as well as biochemical analyses. By combining simple fabrication techniques with adjustable control parameters, the stretcher is designed to fit a variety of experimental needs. The stretcher can be used for static and cyclic stretching. As a proof of principle, we visualize stretch induced deformation of cell nuclei via incremental static stretch, and changes in IEX1 expression via cyclic stretching. This stretcher is easily modified to meet experimental needs, inexpensive to build, and should be readily accessible for most laboratories with access to 3D printing.</p><p> </p></div><h2>Other Information</h2> <p> Published in: Biology Open<br> License: <a href="http://creativecommons.org/licenses/by/4.0" target="_blank">http://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1242/bio.057778" target="_blank">https://dx.doi.org/10.1242/bio.057778</a></p>2021-02-10T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1242/bio.057778https://figshare.com/articles/journal_contribution/Design_of_a_3D_printed_motorized_uniaxial_cell_stretcher_for_microscopic_and_biochemical_analysis_of_mechanotransduction/25764876CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/257648762021-02-10T03:00:00Z |
| spellingShingle | Design of a 3D printed, motorized, uniaxial cell stretcher for microscopic and biochemical analysis of mechanotransduction Noor A. Al-Maslamani (17316925) Biological sciences Biochemistry and cell biology Uniaxial stretcher Mechanobiology 3D printing Cell stretching |
| status_str | publishedVersion |
| title | Design of a 3D printed, motorized, uniaxial cell stretcher for microscopic and biochemical analysis of mechanotransduction |
| title_full | Design of a 3D printed, motorized, uniaxial cell stretcher for microscopic and biochemical analysis of mechanotransduction |
| title_fullStr | Design of a 3D printed, motorized, uniaxial cell stretcher for microscopic and biochemical analysis of mechanotransduction |
| title_full_unstemmed | Design of a 3D printed, motorized, uniaxial cell stretcher for microscopic and biochemical analysis of mechanotransduction |
| title_short | Design of a 3D printed, motorized, uniaxial cell stretcher for microscopic and biochemical analysis of mechanotransduction |
| title_sort | Design of a 3D printed, motorized, uniaxial cell stretcher for microscopic and biochemical analysis of mechanotransduction |
| topic | Biological sciences Biochemistry and cell biology Uniaxial stretcher Mechanobiology 3D printing Cell stretching |