Tipping the Balance: Vitamin D Inadequacy in Children Impacts the Major Gut Bacterial Phyla
<div><p>Vitamin D inadequacy appears to be on the rise globally, and it has been linked to an increased risk of osteoporosis, as well as metabolic, cardiovascular, and autoimmune diseases. Vitamin D concentrations are partially determined by genetic factors. Specific single nucleotide po...
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2022
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| _version_ | 1864513518624047104 |
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| author | Parul Singh (191877) |
| author2 | Arun Rawat (185306) Marwa Saadaoui (8575932) Duaa Elhag (18426882) Sara Tomei (3441323) Mohammed Elanbari (11822375) Anthony K. Akobeng (7713470) Amira Mustafa (18426885) Ibtihal Abdelgadir (18426888) Sharda Udassi (18282271) Mohammed A. Hendaus (18426891) Souhaila Al Khodor (89983) |
| author2_role | author author author author author author author author author author author |
| author_facet | Parul Singh (191877) Arun Rawat (185306) Marwa Saadaoui (8575932) Duaa Elhag (18426882) Sara Tomei (3441323) Mohammed Elanbari (11822375) Anthony K. Akobeng (7713470) Amira Mustafa (18426885) Ibtihal Abdelgadir (18426888) Sharda Udassi (18282271) Mohammed A. Hendaus (18426891) Souhaila Al Khodor (89983) |
| author_role | author |
| dc.creator.none.fl_str_mv | Parul Singh (191877) Arun Rawat (185306) Marwa Saadaoui (8575932) Duaa Elhag (18426882) Sara Tomei (3441323) Mohammed Elanbari (11822375) Anthony K. Akobeng (7713470) Amira Mustafa (18426885) Ibtihal Abdelgadir (18426888) Sharda Udassi (18282271) Mohammed A. Hendaus (18426891) Souhaila Al Khodor (89983) |
| dc.date.none.fl_str_mv | 2022-01-26T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3390/biomedicines10020278 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Tipping_the_Balance_Vitamin_D_Inadequacy_in_Children_Impacts_the_Major_Gut_Bacterial_Phyla/25671636 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Biochemistry and cell biology Genetics pediatric vitamin D deficiency host genetics gut microbiota Qatar Bacteroidetes to Firmicutes ratio |
| dc.title.none.fl_str_mv | Tipping the Balance: Vitamin D Inadequacy in Children Impacts the Major Gut Bacterial Phyla |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <div><p>Vitamin D inadequacy appears to be on the rise globally, and it has been linked to an increased risk of osteoporosis, as well as metabolic, cardiovascular, and autoimmune diseases. Vitamin D concentrations are partially determined by genetic factors. Specific single nucleotide polymorphisms (SNPs) in genes involved in vitamin D transport, metabolism, or binding have been found to be associated with its serum concentration, and these SNPs differ among ethnicities. Vitamin D has also been suggested to be a regulator of the gut microbiota and vitamin D deficiency as the possible cause of gut microbial dysbiosis and inflammation. This pilot study aims to fill the gap in our understanding of the prevalence, cause, and implications of vitamin D inadequacy in a pediatric population residing in Qatar. Blood and fecal samples were collected from healthy subjects aged 4–14 years. Blood was used to measure serum metabolite of vitamin D, 25-hydroxycholecalciferol 25(OH)D. To evaluate the composition of the gut microbiota, fecal samples were subjected to 16S rRNA gene sequencing. High levels of vitamin D deficiency/insufficiency were observed in our cohort with 97% of the subjects falling into the inadequate category (with serum 25(OH)D < 75 nmol/L). The CT genotype in rs12512631, an SNP in the GC gene, was associated with low serum levels of vitamin D (ANOVA, p = 0.0356) and was abundant in deficient compared to non-deficient subjects. Overall gut microbial community structure was significantly different between the deficient (D) and non-deficient (ND) groups (Bray Curtis dissimilarity p = 0.049), with deficient subjects also displaying reduced gut microbial diversity. Significant differences were observed among the two major gut phyla, Firmicutes (F) and Bacteroidetes (B), where deficient subjects displayed a higher B/F ratio (p = 0.0097) compared to ND. Vitamin D deficient children also demonstrated gut enterotypes dominated by the genus Prevotella as opposed to Bacteroides. Our findings suggest that pediatric vitamin D inadequacy significantly impacts the gut microbiota. We also highlight the importance of considering host genetics and baseline gut microbiome composition in interpreting the clinical outcomes related to vitamin D deficiency as well as designing better personalized strategies for therapeutic interventions.</p><p> </p></div><h2>Other Information</h2> <p> Published in: Biomedicines<br> License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/biomedicines10020278" target="_blank">https://dx.doi.org/10.3390/biomedicines10020278</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_ee3c7299ba43f9f851cb23e747d9cb93 |
| identifier_str_mv | 10.3390/biomedicines10020278 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25671636 |
| publishDate | 2022 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Tipping the Balance: Vitamin D Inadequacy in Children Impacts the Major Gut Bacterial PhylaParul Singh (191877)Arun Rawat (185306)Marwa Saadaoui (8575932)Duaa Elhag (18426882)Sara Tomei (3441323)Mohammed Elanbari (11822375)Anthony K. Akobeng (7713470)Amira Mustafa (18426885)Ibtihal Abdelgadir (18426888)Sharda Udassi (18282271)Mohammed A. Hendaus (18426891)Souhaila Al Khodor (89983)Biological sciencesBiochemistry and cell biologyGeneticspediatric vitamin D deficiencyhost geneticsgut microbiotaQatarBacteroidetes to Firmicutes ratio<div><p>Vitamin D inadequacy appears to be on the rise globally, and it has been linked to an increased risk of osteoporosis, as well as metabolic, cardiovascular, and autoimmune diseases. Vitamin D concentrations are partially determined by genetic factors. Specific single nucleotide polymorphisms (SNPs) in genes involved in vitamin D transport, metabolism, or binding have been found to be associated with its serum concentration, and these SNPs differ among ethnicities. Vitamin D has also been suggested to be a regulator of the gut microbiota and vitamin D deficiency as the possible cause of gut microbial dysbiosis and inflammation. This pilot study aims to fill the gap in our understanding of the prevalence, cause, and implications of vitamin D inadequacy in a pediatric population residing in Qatar. Blood and fecal samples were collected from healthy subjects aged 4–14 years. Blood was used to measure serum metabolite of vitamin D, 25-hydroxycholecalciferol 25(OH)D. To evaluate the composition of the gut microbiota, fecal samples were subjected to 16S rRNA gene sequencing. High levels of vitamin D deficiency/insufficiency were observed in our cohort with 97% of the subjects falling into the inadequate category (with serum 25(OH)D < 75 nmol/L). The CT genotype in rs12512631, an SNP in the GC gene, was associated with low serum levels of vitamin D (ANOVA, p = 0.0356) and was abundant in deficient compared to non-deficient subjects. Overall gut microbial community structure was significantly different between the deficient (D) and non-deficient (ND) groups (Bray Curtis dissimilarity p = 0.049), with deficient subjects also displaying reduced gut microbial diversity. Significant differences were observed among the two major gut phyla, Firmicutes (F) and Bacteroidetes (B), where deficient subjects displayed a higher B/F ratio (p = 0.0097) compared to ND. Vitamin D deficient children also demonstrated gut enterotypes dominated by the genus Prevotella as opposed to Bacteroides. Our findings suggest that pediatric vitamin D inadequacy significantly impacts the gut microbiota. We also highlight the importance of considering host genetics and baseline gut microbiome composition in interpreting the clinical outcomes related to vitamin D deficiency as well as designing better personalized strategies for therapeutic interventions.</p><p> </p></div><h2>Other Information</h2> <p> Published in: Biomedicines<br> License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/biomedicines10020278" target="_blank">https://dx.doi.org/10.3390/biomedicines10020278</a></p>2022-01-26T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3390/biomedicines10020278https://figshare.com/articles/journal_contribution/Tipping_the_Balance_Vitamin_D_Inadequacy_in_Children_Impacts_the_Major_Gut_Bacterial_Phyla/25671636CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/256716362022-01-26T03:00:00Z |
| spellingShingle | Tipping the Balance: Vitamin D Inadequacy in Children Impacts the Major Gut Bacterial Phyla Parul Singh (191877) Biological sciences Biochemistry and cell biology Genetics pediatric vitamin D deficiency host genetics gut microbiota Qatar Bacteroidetes to Firmicutes ratio |
| status_str | publishedVersion |
| title | Tipping the Balance: Vitamin D Inadequacy in Children Impacts the Major Gut Bacterial Phyla |
| title_full | Tipping the Balance: Vitamin D Inadequacy in Children Impacts the Major Gut Bacterial Phyla |
| title_fullStr | Tipping the Balance: Vitamin D Inadequacy in Children Impacts the Major Gut Bacterial Phyla |
| title_full_unstemmed | Tipping the Balance: Vitamin D Inadequacy in Children Impacts the Major Gut Bacterial Phyla |
| title_short | Tipping the Balance: Vitamin D Inadequacy in Children Impacts the Major Gut Bacterial Phyla |
| title_sort | Tipping the Balance: Vitamin D Inadequacy in Children Impacts the Major Gut Bacterial Phyla |
| topic | Biological sciences Biochemistry and cell biology Genetics pediatric vitamin D deficiency host genetics gut microbiota Qatar Bacteroidetes to Firmicutes ratio |