Discrimination of breast cancer from benign tumours using Raman spectroscopy
<p dir="ltr">Breast cancer is the most common cancer among women worldwide, with an estimated 1.7 million cases and 522,000 deaths in 2012. Breast cancer is diagnosed by histopathological examination of breast biopsy material but this is subjective and relies on morphological changes...
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2019
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| author | Fiona M. Lyng (2189614) |
| author2 | Damien Traynor (6336077) Thi Nguyet Que Nguyen (6336080) Aidan D. Meade (9191402) Fazle Rakib (5768069) Rafif Al-Saady (5178128) Erik Goormaghtigh (659306) Khalid Al-Saad (5768084) Mohamed H. Ali (3875164) |
| author2_role | author author author author author author author author |
| author_facet | Fiona M. Lyng (2189614) Damien Traynor (6336077) Thi Nguyet Que Nguyen (6336080) Aidan D. Meade (9191402) Fazle Rakib (5768069) Rafif Al-Saady (5178128) Erik Goormaghtigh (659306) Khalid Al-Saad (5768084) Mohamed H. Ali (3875164) |
| author_role | author |
| dc.creator.none.fl_str_mv | Fiona M. Lyng (2189614) Damien Traynor (6336077) Thi Nguyet Que Nguyen (6336080) Aidan D. Meade (9191402) Fazle Rakib (5768069) Rafif Al-Saady (5178128) Erik Goormaghtigh (659306) Khalid Al-Saad (5768084) Mohamed H. Ali (3875164) |
| dc.date.none.fl_str_mv | 2019-02-14T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pone.0212376 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Discrimination_of_breast_cancer_from_benign_tumours_using_Raman_spectroscopy/25333711 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Oncology and carcinogenesis breast cancer tumours Raman spectroscopy women |
| dc.title.none.fl_str_mv | Discrimination of breast cancer from benign tumours using Raman spectroscopy |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Breast cancer is the most common cancer among women worldwide, with an estimated 1.7 million cases and 522,000 deaths in 2012. Breast cancer is diagnosed by histopathological examination of breast biopsy material but this is subjective and relies on morphological changes in the tissue. Raman spectroscopy uses incident radiation to induce vibrations in the molecules of a sample and the scattered radiation can be used to characterise the sample. This technique is rapid and non-destructive and is sensitive to subtle biochemical changes occurring at the molecular level. This allows spectral variations corresponding to disease onset to be detected. The aim of this work was to use Raman spectroscopy to discriminate between benign lesions (fibrocystic, fibroadenoma, intraductal papilloma) and cancer (invasive ductal carcinoma and lobular carcinoma) using formalin fixed paraffin preserved (FFPP) tissue. Haematoxylin and Eosin stained sections from the patient biopsies were marked by a pathologist. Raman maps were recorded from parallel unstained tissue sections. Immunohistochemical staining for estrogen receptor (ER) and human epidermal growth factor receptor 2 <i>(</i>HER2/neu) was performed on a further set of parallel sections. Both benign and cancer cases were positive for ER while only the cancer cases were positive for HER2. Significant spectral differences were observed between the benign and cancer cases and the benign cases could be differentiated from the cancer cases with good sensitivity and specificity. This study has shown the potential of Raman spectroscopy as an aid to histopathological diagnosis of breast cancer, in particular in the discrimination between benign and malignant tumours.</p><h2>Other Information</h2><p dir="ltr">Published in: PLOS ONE<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1371/journal.pone.0212376" target="_blank">https://dx.doi.org/10.1371/journal.pone.0212376</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_f2d98d4b5a2265b564a640834d959b25 |
| identifier_str_mv | 10.1371/journal.pone.0212376 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25333711 |
| publishDate | 2019 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Discrimination of breast cancer from benign tumours using Raman spectroscopyFiona M. Lyng (2189614)Damien Traynor (6336077)Thi Nguyet Que Nguyen (6336080)Aidan D. Meade (9191402)Fazle Rakib (5768069)Rafif Al-Saady (5178128)Erik Goormaghtigh (659306)Khalid Al-Saad (5768084)Mohamed H. Ali (3875164)Biomedical and clinical sciencesOncology and carcinogenesisbreast cancertumoursRaman spectroscopywomen<p dir="ltr">Breast cancer is the most common cancer among women worldwide, with an estimated 1.7 million cases and 522,000 deaths in 2012. Breast cancer is diagnosed by histopathological examination of breast biopsy material but this is subjective and relies on morphological changes in the tissue. Raman spectroscopy uses incident radiation to induce vibrations in the molecules of a sample and the scattered radiation can be used to characterise the sample. This technique is rapid and non-destructive and is sensitive to subtle biochemical changes occurring at the molecular level. This allows spectral variations corresponding to disease onset to be detected. The aim of this work was to use Raman spectroscopy to discriminate between benign lesions (fibrocystic, fibroadenoma, intraductal papilloma) and cancer (invasive ductal carcinoma and lobular carcinoma) using formalin fixed paraffin preserved (FFPP) tissue. Haematoxylin and Eosin stained sections from the patient biopsies were marked by a pathologist. Raman maps were recorded from parallel unstained tissue sections. Immunohistochemical staining for estrogen receptor (ER) and human epidermal growth factor receptor 2 <i>(</i>HER2/neu) was performed on a further set of parallel sections. Both benign and cancer cases were positive for ER while only the cancer cases were positive for HER2. Significant spectral differences were observed between the benign and cancer cases and the benign cases could be differentiated from the cancer cases with good sensitivity and specificity. This study has shown the potential of Raman spectroscopy as an aid to histopathological diagnosis of breast cancer, in particular in the discrimination between benign and malignant tumours.</p><h2>Other Information</h2><p dir="ltr">Published in: PLOS ONE<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1371/journal.pone.0212376" target="_blank">https://dx.doi.org/10.1371/journal.pone.0212376</a></p>2019-02-14T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1371/journal.pone.0212376https://figshare.com/articles/journal_contribution/Discrimination_of_breast_cancer_from_benign_tumours_using_Raman_spectroscopy/25333711CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/253337112019-02-14T03:00:00Z |
| spellingShingle | Discrimination of breast cancer from benign tumours using Raman spectroscopy Fiona M. Lyng (2189614) Biomedical and clinical sciences Oncology and carcinogenesis breast cancer tumours Raman spectroscopy women |
| status_str | publishedVersion |
| title | Discrimination of breast cancer from benign tumours using Raman spectroscopy |
| title_full | Discrimination of breast cancer from benign tumours using Raman spectroscopy |
| title_fullStr | Discrimination of breast cancer from benign tumours using Raman spectroscopy |
| title_full_unstemmed | Discrimination of breast cancer from benign tumours using Raman spectroscopy |
| title_short | Discrimination of breast cancer from benign tumours using Raman spectroscopy |
| title_sort | Discrimination of breast cancer from benign tumours using Raman spectroscopy |
| topic | Biomedical and clinical sciences Oncology and carcinogenesis breast cancer tumours Raman spectroscopy women |