Comparative Study on Beneficial Effects of Hydroxytyrosol- and Oleuropein-Rich Olive Leaf Extracts on High-Fat Diet-Induced Lipid Metabolism Disturbance and Liver Injury in Rats

<p>Oleuropein and hydroxytyrosol, as major compounds of olive leaves, have been reported to exert numerous pharmacological properties, including anticancer, antidiabetic, and anti-inflammatory activities. The purpose of this study is to evaluate and compare the protective effect of oleuropein-...

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Main Author: Ines Fki (14571855) (author)
Other Authors: Sami Sayadi (14571861) (author), Asma Mahmoudi (12996226) (author), Ines Daoued (14571864) (author), Rim Marrekchi (14571870) (author), Hela Ghorbel (14571876) (author)
Published: 2020
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_version_ 1864513563301773312
author Ines Fki (14571855)
author2 Sami Sayadi (14571861)
Asma Mahmoudi (12996226)
Ines Daoued (14571864)
Rim Marrekchi (14571870)
Hela Ghorbel (14571876)
author2_role author
author
author
author
author
author_facet Ines Fki (14571855)
Sami Sayadi (14571861)
Asma Mahmoudi (12996226)
Ines Daoued (14571864)
Rim Marrekchi (14571870)
Hela Ghorbel (14571876)
author_role author
dc.creator.none.fl_str_mv Ines Fki (14571855)
Sami Sayadi (14571861)
Asma Mahmoudi (12996226)
Ines Daoued (14571864)
Rim Marrekchi (14571870)
Hela Ghorbel (14571876)
dc.date.none.fl_str_mv 2020-01-08T18:00:00Z
dc.identifier.none.fl_str_mv 10.1155/2020/1315202
dc.relation.none.fl_str_mv https://figshare.com/articles/journal_contribution/Comparative_Study_on_Beneficial_Effects_of_Hydroxytyrosol-_and_Oleuropein-Rich_Olive_Leaf_Extracts_on_High-Fat_Diet-Induced_Lipid_Metabolism_Disturbance_and_Liver_Injury_in_Rats/22015667
dc.rights.none.fl_str_mv CC BY 4.0
info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Biomedical and clinical sciences
Immunology
Nutrition and dietetics
Pharmacology and pharmaceutical sciences
Oleuropein
Hydroxytyrosol
Olive leaves
Pharmacological properties
Anticancer
Antidiabetic
Anti-inflammatory
dc.title.none.fl_str_mv Comparative Study on Beneficial Effects of Hydroxytyrosol- and Oleuropein-Rich Olive Leaf Extracts on High-Fat Diet-Induced Lipid Metabolism Disturbance and Liver Injury in Rats
dc.type.none.fl_str_mv Text
Journal contribution
info:eu-repo/semantics/publishedVersion
text
contribution to journal
description <p>Oleuropein and hydroxytyrosol, as major compounds of olive leaves, have been reported to exert numerous pharmacological properties, including anticancer, antidiabetic, and anti-inflammatory activities. The purpose of this study is to evaluate and compare the protective effect of oleuropein- and hydroxytyrosol-rich extracts, derived from olive leaves, on high-fat diet-induced lipid metabolism disturbance and liver injury in rats. In this respect, four groups of male rats (8 per group) were used: control group (Control), group treated with high-fat diet (HFD), group treated with HFD and oleuropein (HFD + OLE), and group treated with HFD and hydroxytyrosol (HFD + HYD). The current research showed that the treatment with the HFD increased the body weight and adipose tissue mass in male rats. Moreover, the plasma levels of triglycerides, total cholesterol, LDL-cholesterol, AST, ALT, LDH, and TNF-<em>α</em> were also raised. The hepatic immunohistochemical analysis revealed a significant increase in the expression of inflammatory genes (COX-2, NF-<em>κ</em>B, and TNF-<em>α</em>). Equally, it showed a rise of the apoptotic markers (a decrease in the expression of the Bcl-2 and an increase of the P53). In addition, the oral administration of oleuropein- and hydroxytyrosol-rich olive leaf extracts at 16 mg/kg similarly reduced the body weight and adipose tissue mass and improved the lipid profile. Moreover, these extracts, mainly the hydroxytyrosol-rich extract, reduced the elevated liver enzymes, enhanced the antioxidant status, and attenuated the liver inflammation and apoptosis. These findings suggest that the oleuropein- and hydroxytyrosol-rich olive leaf extracts possessed hypolipidemic and hepatoprotective effects against the HFD-induced metabolic disorders by enhancing the antioxidative defense system and blocking the expression of the proteins involved in inflammation and liver damage. </p> <h2>Other information </h2> <p>Published in: BioMed Research International<br> License: <a href="http://creativecommons.org/licenses/by/4.0" target="_blank">http://creativecommons.org/licenses/by/4.0</a><br> See article on publisher's website: <a href="http://dx.doi.org/10.1155/2020/1315202" target="_blank">http://dx.doi.org/10.1155/2020/1315202</a></p>
eu_rights_str_mv openAccess
id Manara2_f4c65b90601574fa0eb32206a7493cd9
identifier_str_mv 10.1155/2020/1315202
network_acronym_str Manara2
network_name_str Manara2
oai_identifier_str oai:figshare.com:article/22015667
publishDate 2020
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rights_invalid_str_mv CC BY 4.0
spelling Comparative Study on Beneficial Effects of Hydroxytyrosol- and Oleuropein-Rich Olive Leaf Extracts on High-Fat Diet-Induced Lipid Metabolism Disturbance and Liver Injury in RatsInes Fki (14571855)Sami Sayadi (14571861)Asma Mahmoudi (12996226)Ines Daoued (14571864)Rim Marrekchi (14571870)Hela Ghorbel (14571876)Biomedical and clinical sciencesImmunologyNutrition and dieteticsPharmacology and pharmaceutical sciencesOleuropeinHydroxytyrosolOlive leavesPharmacological propertiesAnticancerAntidiabeticAnti-inflammatory<p>Oleuropein and hydroxytyrosol, as major compounds of olive leaves, have been reported to exert numerous pharmacological properties, including anticancer, antidiabetic, and anti-inflammatory activities. The purpose of this study is to evaluate and compare the protective effect of oleuropein- and hydroxytyrosol-rich extracts, derived from olive leaves, on high-fat diet-induced lipid metabolism disturbance and liver injury in rats. In this respect, four groups of male rats (8 per group) were used: control group (Control), group treated with high-fat diet (HFD), group treated with HFD and oleuropein (HFD + OLE), and group treated with HFD and hydroxytyrosol (HFD + HYD). The current research showed that the treatment with the HFD increased the body weight and adipose tissue mass in male rats. Moreover, the plasma levels of triglycerides, total cholesterol, LDL-cholesterol, AST, ALT, LDH, and TNF-<em>α</em> were also raised. The hepatic immunohistochemical analysis revealed a significant increase in the expression of inflammatory genes (COX-2, NF-<em>κ</em>B, and TNF-<em>α</em>). Equally, it showed a rise of the apoptotic markers (a decrease in the expression of the Bcl-2 and an increase of the P53). In addition, the oral administration of oleuropein- and hydroxytyrosol-rich olive leaf extracts at 16 mg/kg similarly reduced the body weight and adipose tissue mass and improved the lipid profile. Moreover, these extracts, mainly the hydroxytyrosol-rich extract, reduced the elevated liver enzymes, enhanced the antioxidant status, and attenuated the liver inflammation and apoptosis. These findings suggest that the oleuropein- and hydroxytyrosol-rich olive leaf extracts possessed hypolipidemic and hepatoprotective effects against the HFD-induced metabolic disorders by enhancing the antioxidative defense system and blocking the expression of the proteins involved in inflammation and liver damage. </p> <h2>Other information </h2> <p>Published in: BioMed Research International<br> License: <a href="http://creativecommons.org/licenses/by/4.0" target="_blank">http://creativecommons.org/licenses/by/4.0</a><br> See article on publisher's website: <a href="http://dx.doi.org/10.1155/2020/1315202" target="_blank">http://dx.doi.org/10.1155/2020/1315202</a></p>2020-01-08T18:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1155/2020/1315202https://figshare.com/articles/journal_contribution/Comparative_Study_on_Beneficial_Effects_of_Hydroxytyrosol-_and_Oleuropein-Rich_Olive_Leaf_Extracts_on_High-Fat_Diet-Induced_Lipid_Metabolism_Disturbance_and_Liver_Injury_in_Rats/22015667CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/220156672020-01-08T18:00:00Z
spellingShingle Comparative Study on Beneficial Effects of Hydroxytyrosol- and Oleuropein-Rich Olive Leaf Extracts on High-Fat Diet-Induced Lipid Metabolism Disturbance and Liver Injury in Rats
Ines Fki (14571855)
Biomedical and clinical sciences
Immunology
Nutrition and dietetics
Pharmacology and pharmaceutical sciences
Oleuropein
Hydroxytyrosol
Olive leaves
Pharmacological properties
Anticancer
Antidiabetic
Anti-inflammatory
status_str publishedVersion
title Comparative Study on Beneficial Effects of Hydroxytyrosol- and Oleuropein-Rich Olive Leaf Extracts on High-Fat Diet-Induced Lipid Metabolism Disturbance and Liver Injury in Rats
title_full Comparative Study on Beneficial Effects of Hydroxytyrosol- and Oleuropein-Rich Olive Leaf Extracts on High-Fat Diet-Induced Lipid Metabolism Disturbance and Liver Injury in Rats
title_fullStr Comparative Study on Beneficial Effects of Hydroxytyrosol- and Oleuropein-Rich Olive Leaf Extracts on High-Fat Diet-Induced Lipid Metabolism Disturbance and Liver Injury in Rats
title_full_unstemmed Comparative Study on Beneficial Effects of Hydroxytyrosol- and Oleuropein-Rich Olive Leaf Extracts on High-Fat Diet-Induced Lipid Metabolism Disturbance and Liver Injury in Rats
title_short Comparative Study on Beneficial Effects of Hydroxytyrosol- and Oleuropein-Rich Olive Leaf Extracts on High-Fat Diet-Induced Lipid Metabolism Disturbance and Liver Injury in Rats
title_sort Comparative Study on Beneficial Effects of Hydroxytyrosol- and Oleuropein-Rich Olive Leaf Extracts on High-Fat Diet-Induced Lipid Metabolism Disturbance and Liver Injury in Rats
topic Biomedical and clinical sciences
Immunology
Nutrition and dietetics
Pharmacology and pharmaceutical sciences
Oleuropein
Hydroxytyrosol
Olive leaves
Pharmacological properties
Anticancer
Antidiabetic
Anti-inflammatory