Effect of nutrients deficiency on biofilm formation and single cell protein production with a purple non-sulphur bacteria enriched culture
<p>Purple non-sulphur bacteria (PNSB) are of interest for biorefinery applications to create biomolecules, but their production cost is expensive due to substrate and biomass separation costs. This research has utilized fuel synthesis wastewater (FSW) as a low-cost carbon-rich substrate to pro...
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| مؤلفون آخرون: | , , , |
| منشور في: |
2023
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| _version_ | 1864513507597221888 |
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| author | S. Shaikh (17337769) |
| author2 | N. Rashid (17337772) U. Onwusogh (19457383) G. McKay (14152104) H.R. Mackey (14152101) |
| author2_role | author author author author |
| author_facet | S. Shaikh (17337769) N. Rashid (17337772) U. Onwusogh (19457383) G. McKay (14152104) H.R. Mackey (14152101) |
| author_role | author |
| dc.creator.none.fl_str_mv | S. Shaikh (17337769) N. Rashid (17337772) U. Onwusogh (19457383) G. McKay (14152104) H.R. Mackey (14152101) |
| dc.date.none.fl_str_mv | 2023-12-19T09:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1016/j.bioflm.2022.100098 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Effect_of_nutrients_deficiency_on_biofilm_formation_and_single_cell_protein_production_with_a_purple_non-sulphur_bacteria_enriched_culture/26796223 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Microbiology Engineering Fluid mechanics and thermal engineering Fuel-synthesis wastewater Purple non-sulphur bacteria Single cell protein Biofilm Nutrients deficiency |
| dc.title.none.fl_str_mv | Effect of nutrients deficiency on biofilm formation and single cell protein production with a purple non-sulphur bacteria enriched culture |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p>Purple non-sulphur bacteria (PNSB) are of interest for biorefinery applications to create biomolecules, but their production cost is expensive due to substrate and biomass separation costs. This research has utilized fuel synthesis wastewater (FSW) as a low-cost carbon-rich substrate to produce single-cell protein (SCP) and examines PNSB biofilm formation using this substrate to achieve a more efficient biomass-liquid separation. In this study, PNSB were grown in Ca, Mg, S, P, and N-deficient media using green shade as biofilm support material. Among these nutrient conditions, only N-deficient and control (nutrient-sufficient) conditions showed biofilm formation. Although total biomass growth of the control was 1.5 times that of the N-deficient condition and highest overall, the total biofilm-biomass in the N-deficient condition was 2.5 times greater than the control, comprising 49% of total biomass produced. Total protein content was similar between these four biomass samples, ranging from 35.0 ± 0.2% to 37.2 ± 0.0%. The highest protein content of 44.7 ± 1.3% occurred in the Mg-deficient condition (suspended biomass only) but suffered from a low growth rate. Overall, nutrient sufficient conditions are optimal for overall protein productivity and dominated by suspended growth, but where fixed growth systems are desired for cost-effective harvesting, N-deficient conditions provide an effective means to maximize biofilm production without sacrificing protein content.</p><h2>Other Information</h2> <p> Published in: Biofilm<br> License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.bioflm.2022.100098" target="_blank">https://dx.doi.org/10.1016/j.bioflm.2022.100098</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_f55b228464cc90089ce28a3a193d6cc7 |
| identifier_str_mv | 10.1016/j.bioflm.2022.100098 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/26796223 |
| publishDate | 2023 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Effect of nutrients deficiency on biofilm formation and single cell protein production with a purple non-sulphur bacteria enriched cultureS. Shaikh (17337769)N. Rashid (17337772)U. Onwusogh (19457383)G. McKay (14152104)H.R. Mackey (14152101)Biological sciencesMicrobiologyEngineeringFluid mechanics and thermal engineeringFuel-synthesis wastewaterPurple non-sulphur bacteriaSingle cell proteinBiofilmNutrients deficiency<p>Purple non-sulphur bacteria (PNSB) are of interest for biorefinery applications to create biomolecules, but their production cost is expensive due to substrate and biomass separation costs. This research has utilized fuel synthesis wastewater (FSW) as a low-cost carbon-rich substrate to produce single-cell protein (SCP) and examines PNSB biofilm formation using this substrate to achieve a more efficient biomass-liquid separation. In this study, PNSB were grown in Ca, Mg, S, P, and N-deficient media using green shade as biofilm support material. Among these nutrient conditions, only N-deficient and control (nutrient-sufficient) conditions showed biofilm formation. Although total biomass growth of the control was 1.5 times that of the N-deficient condition and highest overall, the total biofilm-biomass in the N-deficient condition was 2.5 times greater than the control, comprising 49% of total biomass produced. Total protein content was similar between these four biomass samples, ranging from 35.0 ± 0.2% to 37.2 ± 0.0%. The highest protein content of 44.7 ± 1.3% occurred in the Mg-deficient condition (suspended biomass only) but suffered from a low growth rate. Overall, nutrient sufficient conditions are optimal for overall protein productivity and dominated by suspended growth, but where fixed growth systems are desired for cost-effective harvesting, N-deficient conditions provide an effective means to maximize biofilm production without sacrificing protein content.</p><h2>Other Information</h2> <p> Published in: Biofilm<br> License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.bioflm.2022.100098" target="_blank">https://dx.doi.org/10.1016/j.bioflm.2022.100098</a></p>2023-12-19T09:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1016/j.bioflm.2022.100098https://figshare.com/articles/journal_contribution/Effect_of_nutrients_deficiency_on_biofilm_formation_and_single_cell_protein_production_with_a_purple_non-sulphur_bacteria_enriched_culture/26796223CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/267962232023-12-19T09:00:00Z |
| spellingShingle | Effect of nutrients deficiency on biofilm formation and single cell protein production with a purple non-sulphur bacteria enriched culture S. Shaikh (17337769) Biological sciences Microbiology Engineering Fluid mechanics and thermal engineering Fuel-synthesis wastewater Purple non-sulphur bacteria Single cell protein Biofilm Nutrients deficiency |
| status_str | publishedVersion |
| title | Effect of nutrients deficiency on biofilm formation and single cell protein production with a purple non-sulphur bacteria enriched culture |
| title_full | Effect of nutrients deficiency on biofilm formation and single cell protein production with a purple non-sulphur bacteria enriched culture |
| title_fullStr | Effect of nutrients deficiency on biofilm formation and single cell protein production with a purple non-sulphur bacteria enriched culture |
| title_full_unstemmed | Effect of nutrients deficiency on biofilm formation and single cell protein production with a purple non-sulphur bacteria enriched culture |
| title_short | Effect of nutrients deficiency on biofilm formation and single cell protein production with a purple non-sulphur bacteria enriched culture |
| title_sort | Effect of nutrients deficiency on biofilm formation and single cell protein production with a purple non-sulphur bacteria enriched culture |
| topic | Biological sciences Microbiology Engineering Fluid mechanics and thermal engineering Fuel-synthesis wastewater Purple non-sulphur bacteria Single cell protein Biofilm Nutrients deficiency |