Phosphatidylinositol-4,5-Biphosphate (PI(4,5)P<sub>2</sub>) Is Required for Rapid Endocytosis in Chromaffin Cells
<p><em>Objective.</em> Phosphoinositides play a regulatory role in clathrin-mediated endocytosis. However, their involvement in clathrin-independent endocytosis termed rapid endocytosis (RE), which is the mode of vesicle recycling during neurotransmitter release by transient fusion...
محفوظ في:
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| منشور في: |
2020
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| الملخص: | <p><em>Objective.</em> Phosphoinositides play a regulatory role in clathrin-mediated endocytosis. However, their involvement in clathrin-independent endocytosis termed rapid endocytosis (RE), which is the mode of vesicle recycling during neurotransmitter release by transient fusion (known as kiss-and-run), has not been investigated. Here, we used patch-clamp recording of whole-cell membrane capacitance in adrenal chromaffin cells (ACC) to monitor changes of RE kinetics in response to pharmacological alteration of phosphatidylinositol-4,5-biphosphate (PI(4,5)P<sub>2</sub>) level by phenylarsine oxide (PAO) or antibody against phosphatidylinositol 4-kinase (Ab<sub>PI4K</sub>). <em>Results.</em> We found that PAO and Ab<sub>PI4K</sub> significantly abrogated RE kinetics. Infusion of PI(4,5)P<sub>2</sub> through the patch pipette potentiated RE kinetics and reversed PAO- and Ab<sub>PI4K</sub>-induced blockade of RE. Similarly, the application of the bifunctional thiol dithiothreitol (DTT) to PAO-treated cells completely prevented the inhibitory effect of PAO on RE. These findings indicate that PI(4,5)P<sub>2</sub> is implicated in the signaling (mechanistic) process of RE in ACC.</p> <h2>Other information </h2> <p>Published in: BioMed Research International<br> License: <a href="http://creativecommons.org/licenses/by/4.0" target="_blank">http://creativecommons.org/licenses/by/4.0</a><br> See article on publisher's website: <a href="http://dx.doi.org/10.1155/2020/9692503" target="_blank">http://dx.doi.org/10.1155/2020/9692503</a></p> |
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