Phosphatidylinositol-4,5-Biphosphate (PI(4,5)P<sub>2</sub>) Is Required for Rapid Endocytosis in Chromaffin Cells
<p><em>Objective.</em> Phosphoinositides play a regulatory role in clathrin-mediated endocytosis. However, their involvement in clathrin-independent endocytosis termed rapid endocytosis (RE), which is the mode of vesicle recycling during neurotransmitter release by transient fusion...
محفوظ في:
| المؤلف الرئيسي: | |
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| منشور في: |
2020
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| الموضوعات: | |
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| _version_ | 1864513563326939136 |
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| author | Fouad Azizi (14152740) |
| author_facet | Fouad Azizi (14152740) |
| author_role | author |
| dc.creator.none.fl_str_mv | Fouad Azizi (14152740) |
| dc.date.none.fl_str_mv | 2020-09-10T21:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1155/2020/9692503 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Phosphatidylinositol-4_5-Biphosphate_PI_4_5_P_sub_2_sub_Is_Required_for_Rapid_Endocytosis_in_Chromaffin_Cells/22014407 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Medical biochemistry and metabolomics General Immunology and Microbiology General Biochemistry Genetics and Molecular Biology General Medicine |
| dc.title.none.fl_str_mv | Phosphatidylinositol-4,5-Biphosphate (PI(4,5)P<sub>2</sub>) Is Required for Rapid Endocytosis in Chromaffin Cells |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p><em>Objective.</em> Phosphoinositides play a regulatory role in clathrin-mediated endocytosis. However, their involvement in clathrin-independent endocytosis termed rapid endocytosis (RE), which is the mode of vesicle recycling during neurotransmitter release by transient fusion (known as kiss-and-run), has not been investigated. Here, we used patch-clamp recording of whole-cell membrane capacitance in adrenal chromaffin cells (ACC) to monitor changes of RE kinetics in response to pharmacological alteration of phosphatidylinositol-4,5-biphosphate (PI(4,5)P<sub>2</sub>) level by phenylarsine oxide (PAO) or antibody against phosphatidylinositol 4-kinase (Ab<sub>PI4K</sub>). <em>Results.</em> We found that PAO and Ab<sub>PI4K</sub> significantly abrogated RE kinetics. Infusion of PI(4,5)P<sub>2</sub> through the patch pipette potentiated RE kinetics and reversed PAO- and Ab<sub>PI4K</sub>-induced blockade of RE. Similarly, the application of the bifunctional thiol dithiothreitol (DTT) to PAO-treated cells completely prevented the inhibitory effect of PAO on RE. These findings indicate that PI(4,5)P<sub>2</sub> is implicated in the signaling (mechanistic) process of RE in ACC.</p> <h2>Other information </h2> <p>Published in: BioMed Research International<br> License: <a href="http://creativecommons.org/licenses/by/4.0" target="_blank">http://creativecommons.org/licenses/by/4.0</a><br> See article on publisher's website: <a href="http://dx.doi.org/10.1155/2020/9692503" target="_blank">http://dx.doi.org/10.1155/2020/9692503</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_f68d417c760aca99e7c610b4dcc44b43 |
| identifier_str_mv | 10.1155/2020/9692503 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/22014407 |
| publishDate | 2020 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Phosphatidylinositol-4,5-Biphosphate (PI(4,5)P<sub>2</sub>) Is Required for Rapid Endocytosis in Chromaffin CellsFouad Azizi (14152740)Biomedical and clinical sciencesMedical biochemistry and metabolomicsGeneral Immunology and MicrobiologyGeneral BiochemistryGenetics and Molecular BiologyGeneral Medicine<p><em>Objective.</em> Phosphoinositides play a regulatory role in clathrin-mediated endocytosis. However, their involvement in clathrin-independent endocytosis termed rapid endocytosis (RE), which is the mode of vesicle recycling during neurotransmitter release by transient fusion (known as kiss-and-run), has not been investigated. Here, we used patch-clamp recording of whole-cell membrane capacitance in adrenal chromaffin cells (ACC) to monitor changes of RE kinetics in response to pharmacological alteration of phosphatidylinositol-4,5-biphosphate (PI(4,5)P<sub>2</sub>) level by phenylarsine oxide (PAO) or antibody against phosphatidylinositol 4-kinase (Ab<sub>PI4K</sub>). <em>Results.</em> We found that PAO and Ab<sub>PI4K</sub> significantly abrogated RE kinetics. Infusion of PI(4,5)P<sub>2</sub> through the patch pipette potentiated RE kinetics and reversed PAO- and Ab<sub>PI4K</sub>-induced blockade of RE. Similarly, the application of the bifunctional thiol dithiothreitol (DTT) to PAO-treated cells completely prevented the inhibitory effect of PAO on RE. These findings indicate that PI(4,5)P<sub>2</sub> is implicated in the signaling (mechanistic) process of RE in ACC.</p> <h2>Other information </h2> <p>Published in: BioMed Research International<br> License: <a href="http://creativecommons.org/licenses/by/4.0" target="_blank">http://creativecommons.org/licenses/by/4.0</a><br> See article on publisher's website: <a href="http://dx.doi.org/10.1155/2020/9692503" target="_blank">http://dx.doi.org/10.1155/2020/9692503</a></p>2020-09-10T21:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1155/2020/9692503https://figshare.com/articles/journal_contribution/Phosphatidylinositol-4_5-Biphosphate_PI_4_5_P_sub_2_sub_Is_Required_for_Rapid_Endocytosis_in_Chromaffin_Cells/22014407CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/220144072020-09-10T21:00:00Z |
| spellingShingle | Phosphatidylinositol-4,5-Biphosphate (PI(4,5)P<sub>2</sub>) Is Required for Rapid Endocytosis in Chromaffin Cells Fouad Azizi (14152740) Biomedical and clinical sciences Medical biochemistry and metabolomics General Immunology and Microbiology General Biochemistry Genetics and Molecular Biology General Medicine |
| status_str | publishedVersion |
| title | Phosphatidylinositol-4,5-Biphosphate (PI(4,5)P<sub>2</sub>) Is Required for Rapid Endocytosis in Chromaffin Cells |
| title_full | Phosphatidylinositol-4,5-Biphosphate (PI(4,5)P<sub>2</sub>) Is Required for Rapid Endocytosis in Chromaffin Cells |
| title_fullStr | Phosphatidylinositol-4,5-Biphosphate (PI(4,5)P<sub>2</sub>) Is Required for Rapid Endocytosis in Chromaffin Cells |
| title_full_unstemmed | Phosphatidylinositol-4,5-Biphosphate (PI(4,5)P<sub>2</sub>) Is Required for Rapid Endocytosis in Chromaffin Cells |
| title_short | Phosphatidylinositol-4,5-Biphosphate (PI(4,5)P<sub>2</sub>) Is Required for Rapid Endocytosis in Chromaffin Cells |
| title_sort | Phosphatidylinositol-4,5-Biphosphate (PI(4,5)P<sub>2</sub>) Is Required for Rapid Endocytosis in Chromaffin Cells |
| topic | Biomedical and clinical sciences Medical biochemistry and metabolomics General Immunology and Microbiology General Biochemistry Genetics and Molecular Biology General Medicine |