A novel in ovo model to study cancer metastasis using chicken embryos and GFP expressing cancer cells
<p dir="ltr">Cancer metastasis is the leading cause of cancer-related mortality worldwide. To date, several in vitro methodologies have been developed to understand the mechanisms of cancer metastasis and to screen various therapeutic agents against it. Nevertheless, mimicking an in...
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| مؤلفون آخرون: | , , , , |
| منشور في: |
2020
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إضافة وسم
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| _version_ | 1864513523117195264 |
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| author | Robin Augustine (3976964) |
| author2 | Hashim Alhussain (9167136) Anwarul Hasan (1332066) Mohamed Badie Ahmed (16515335) Huseyin C. Yalcin (16442020) Ala-Eddin Al Moustafa (14153205) |
| author2_role | author author author author author |
| author_facet | Robin Augustine (3976964) Hashim Alhussain (9167136) Anwarul Hasan (1332066) Mohamed Badie Ahmed (16515335) Huseyin C. Yalcin (16442020) Ala-Eddin Al Moustafa (14153205) |
| author_role | author |
| dc.creator.none.fl_str_mv | Robin Augustine (3976964) Hashim Alhussain (9167136) Anwarul Hasan (1332066) Mohamed Badie Ahmed (16515335) Huseyin C. Yalcin (16442020) Ala-Eddin Al Moustafa (14153205) |
| dc.date.none.fl_str_mv | 2020-02-05T09:00:00Z |
| dc.identifier.none.fl_str_mv | 10.17305/bjbms.2019.4372 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/A_novel_in_ovo_model_to_study_cancer_metastasis_using_chicken_embryos_and_GFP_expressing_cancer_cells/25341784 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Oncology and carcinogenesis Engineering Biomedical engineering Tumor invasion in ovo models chicken embryo model metastasis GFP cells |
| dc.title.none.fl_str_mv | A novel in ovo model to study cancer metastasis using chicken embryos and GFP expressing cancer cells |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Cancer metastasis is the leading cause of cancer-related mortality worldwide. To date, several in vitro methodologies have been developed to understand the mechanisms of cancer metastasis and to screen various therapeutic agents against it. Nevertheless, mimicking an in vivo microenvironment in vitro is not possible; while in vivo experiments are complex, expensive and bound with several regulatory requirements. Herein, we report a novel in ovo model that relies on chicken embryo to investigate cancer cell invasion and metastasis to various organs of the body. In this model, we directly inject green fluorescent protein (GFP) expressing cancer cells to the heart of chicken embryo at 3 days of incubation, then monitor cell migration to various organs. To this end, we used a simple tissue processing technique to achieve rapid imaging and quantification of invasive cells. We were able to clearly observe the migration of GFP expressing cancer cells into various organs of chicken embryo. Organ specific variation in cell migration was also observed. Our new slide pressing based tissue processing technique improved the detectability of migrated cells. We herein demonstrate that the use of GFP expressing cancer cells allows easy detection and quantification of migrated cancer cells in the chicken embryo model, which minimizes the time and effort required in this types of studies compared to conventional histopathological analysis. In conclusion, our investigation provides a new cancer metastasis model that can be further improved to include more complex aspects, such as the use of multiple cell lines and anti-metastatic agents, thus opening new horizons in cancer biology and pharmaceutical research.</p><h2>Other Information</h2><p dir="ltr">Published in: Bosnian Journal of Basic Medical Sciences<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.17305/bjbms.2019.4372" target="_blank">https://dx.doi.org/10.17305/bjbms.2019.4372</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_f695ff5ce03a1b81973b0b6baf6ec057 |
| identifier_str_mv | 10.17305/bjbms.2019.4372 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25341784 |
| publishDate | 2020 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | A novel in ovo model to study cancer metastasis using chicken embryos and GFP expressing cancer cellsRobin Augustine (3976964)Hashim Alhussain (9167136)Anwarul Hasan (1332066)Mohamed Badie Ahmed (16515335)Huseyin C. Yalcin (16442020)Ala-Eddin Al Moustafa (14153205)Biological sciencesBiochemistry and cell biologyBiomedical and clinical sciencesOncology and carcinogenesisEngineeringBiomedical engineeringTumor invasionin ovo modelschicken embryo modelmetastasisGFP cells<p dir="ltr">Cancer metastasis is the leading cause of cancer-related mortality worldwide. To date, several in vitro methodologies have been developed to understand the mechanisms of cancer metastasis and to screen various therapeutic agents against it. Nevertheless, mimicking an in vivo microenvironment in vitro is not possible; while in vivo experiments are complex, expensive and bound with several regulatory requirements. Herein, we report a novel in ovo model that relies on chicken embryo to investigate cancer cell invasion and metastasis to various organs of the body. In this model, we directly inject green fluorescent protein (GFP) expressing cancer cells to the heart of chicken embryo at 3 days of incubation, then monitor cell migration to various organs. To this end, we used a simple tissue processing technique to achieve rapid imaging and quantification of invasive cells. We were able to clearly observe the migration of GFP expressing cancer cells into various organs of chicken embryo. Organ specific variation in cell migration was also observed. Our new slide pressing based tissue processing technique improved the detectability of migrated cells. We herein demonstrate that the use of GFP expressing cancer cells allows easy detection and quantification of migrated cancer cells in the chicken embryo model, which minimizes the time and effort required in this types of studies compared to conventional histopathological analysis. In conclusion, our investigation provides a new cancer metastasis model that can be further improved to include more complex aspects, such as the use of multiple cell lines and anti-metastatic agents, thus opening new horizons in cancer biology and pharmaceutical research.</p><h2>Other Information</h2><p dir="ltr">Published in: Bosnian Journal of Basic Medical Sciences<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.17305/bjbms.2019.4372" target="_blank">https://dx.doi.org/10.17305/bjbms.2019.4372</a></p>2020-02-05T09:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.17305/bjbms.2019.4372https://figshare.com/articles/journal_contribution/A_novel_in_ovo_model_to_study_cancer_metastasis_using_chicken_embryos_and_GFP_expressing_cancer_cells/25341784CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/253417842020-02-05T09:00:00Z |
| spellingShingle | A novel in ovo model to study cancer metastasis using chicken embryos and GFP expressing cancer cells Robin Augustine (3976964) Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Oncology and carcinogenesis Engineering Biomedical engineering Tumor invasion in ovo models chicken embryo model metastasis GFP cells |
| status_str | publishedVersion |
| title | A novel in ovo model to study cancer metastasis using chicken embryos and GFP expressing cancer cells |
| title_full | A novel in ovo model to study cancer metastasis using chicken embryos and GFP expressing cancer cells |
| title_fullStr | A novel in ovo model to study cancer metastasis using chicken embryos and GFP expressing cancer cells |
| title_full_unstemmed | A novel in ovo model to study cancer metastasis using chicken embryos and GFP expressing cancer cells |
| title_short | A novel in ovo model to study cancer metastasis using chicken embryos and GFP expressing cancer cells |
| title_sort | A novel in ovo model to study cancer metastasis using chicken embryos and GFP expressing cancer cells |
| topic | Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Oncology and carcinogenesis Engineering Biomedical engineering Tumor invasion in ovo models chicken embryo model metastasis GFP cells |