Effect of induced hypoglycemia on inflammation and oxidative stress in type 2 diabetes and control subjects
<p dir="ltr">Intensive diabetes control has been associated with increased mortality in type 2 diabetes (T2DM); this has been suggested to be due to increased hypoglycemia. We measured hypoglycemia-induced changes in endothelial parameters, oxidative stress markers and inflammation a...
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| مؤلفون آخرون: | , , , , , , , |
| منشور في: |
2020
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| _version_ | 1864513512814936064 |
|---|---|
| author | Hassan Kahal (9999461) |
| author2 | Anna Halama (545988) Ahmed Aburima (5181119) Aditya M. Bhagwat (14152806) Alexandra E. Butler (6189536) Johannes Graumann (1297758) Karsten Suhre (67967) Thozhukat Sathyapalan (704787) Stephen L. Atkin (6684368) |
| author2_role | author author author author author author author author |
| author_facet | Hassan Kahal (9999461) Anna Halama (545988) Ahmed Aburima (5181119) Aditya M. Bhagwat (14152806) Alexandra E. Butler (6189536) Johannes Graumann (1297758) Karsten Suhre (67967) Thozhukat Sathyapalan (704787) Stephen L. Atkin (6684368) |
| author_role | author |
| dc.creator.none.fl_str_mv | Hassan Kahal (9999461) Anna Halama (545988) Ahmed Aburima (5181119) Aditya M. Bhagwat (14152806) Alexandra E. Butler (6189536) Johannes Graumann (1297758) Karsten Suhre (67967) Thozhukat Sathyapalan (704787) Stephen L. Atkin (6684368) |
| dc.date.none.fl_str_mv | 2020-03-16T06:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1038/s41598-020-61531-z |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Effect_of_induced_hypoglycemia_on_inflammation_and_oxidative_stress_in_type_2_diabetes_and_control_subjects/21598155 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Clinical sciences Diabetes complications Type 2 diabetes Inflammation Oxidative Stress |
| dc.title.none.fl_str_mv | Effect of induced hypoglycemia on inflammation and oxidative stress in type 2 diabetes and control subjects |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Intensive diabetes control has been associated with increased mortality in type 2 diabetes (T2DM); this has been suggested to be due to increased hypoglycemia. We measured hypoglycemia-induced changes in endothelial parameters, oxidative stress markers and inflammation at baseline and after a 24-hour period in type 2 diabetic (T2DM) subjects versus age-matched controls. Case-control study: 10 T2DM and 8 control subjects. Blood glucose was reduced from 5 (90 mg/dl) to hypoglycemic levels of 2.8 mmol/L (50 mg/dl) for 1 hour by incremental hyperinsulinemic clamps using baseline and 24 hour samples. Measures of endothelial parameters, oxidative stress and inflammation at baseline and at 24-hours post hypoglycemia were performed: proteomic (Somalogic) analysis for inflammatory markers complemented by C-reactive protein (hsCRP) measurement, and proteomic markers and urinary isoprostanes for oxidative measures, together with endothelial function. Between baseline and 24 -hours after hypoglycemia, 15 of 140 inflammatory proteins differed in T2DM whilst only 1 of 140 differed in controls; all returned to baseline at 24-hours. However, elevated hsCRP levels were seen at 24-hours in T2DM (2.4 mg/L (1.2–5.4) vs. 3.9 mg/L (1.8–6.1), Baseline vs 24-hours, P < 0.05). In patients with T2DM, between baseline and 24-hour after hypoglycemia, only one of 15 oxidative stress proteins differed and this was not seen in controls. An increase (P = 0.016) from baseline (73.4 ng/mL) to 24 hours after hypoglycemia (91.7 ng/mL) was seen for urinary isoprostanes. Hypoglycemia resulted in inflammatory and oxidative stress markers being elevated in T2DM subjects but not controls 24-hours after the event.</p><p dir="ltr">Author Correction: Effect of induced hypoglycemia on inflammation and oxidative stress in type 2 diabetes and control subjects: <a href="https://dx.doi.org/10.1038/s41598-020-66189-1" target="_blank">https://dx.doi.org/10.1038/s41598-020-66189-1</a>, published online 19 June 2020.</p><h2>Other Information</h2><p dir="ltr">Published in: Scientific Reports<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="http://dx.doi.org/10.1038/s41598-020-61531-z" target="_blank">http://dx.doi.org/10.1038/s41598-020-61531-z</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_f6db710aa759435a33815b43424a102c |
| identifier_str_mv | 10.1038/s41598-020-61531-z |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/21598155 |
| publishDate | 2020 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Effect of induced hypoglycemia on inflammation and oxidative stress in type 2 diabetes and control subjectsHassan Kahal (9999461)Anna Halama (545988)Ahmed Aburima (5181119)Aditya M. Bhagwat (14152806)Alexandra E. Butler (6189536)Johannes Graumann (1297758)Karsten Suhre (67967)Thozhukat Sathyapalan (704787)Stephen L. Atkin (6684368)Biomedical and clinical sciencesClinical sciencesDiabetes complicationsType 2 diabetesInflammationOxidative Stress<p dir="ltr">Intensive diabetes control has been associated with increased mortality in type 2 diabetes (T2DM); this has been suggested to be due to increased hypoglycemia. We measured hypoglycemia-induced changes in endothelial parameters, oxidative stress markers and inflammation at baseline and after a 24-hour period in type 2 diabetic (T2DM) subjects versus age-matched controls. Case-control study: 10 T2DM and 8 control subjects. Blood glucose was reduced from 5 (90 mg/dl) to hypoglycemic levels of 2.8 mmol/L (50 mg/dl) for 1 hour by incremental hyperinsulinemic clamps using baseline and 24 hour samples. Measures of endothelial parameters, oxidative stress and inflammation at baseline and at 24-hours post hypoglycemia were performed: proteomic (Somalogic) analysis for inflammatory markers complemented by C-reactive protein (hsCRP) measurement, and proteomic markers and urinary isoprostanes for oxidative measures, together with endothelial function. Between baseline and 24 -hours after hypoglycemia, 15 of 140 inflammatory proteins differed in T2DM whilst only 1 of 140 differed in controls; all returned to baseline at 24-hours. However, elevated hsCRP levels were seen at 24-hours in T2DM (2.4 mg/L (1.2–5.4) vs. 3.9 mg/L (1.8–6.1), Baseline vs 24-hours, P < 0.05). In patients with T2DM, between baseline and 24-hour after hypoglycemia, only one of 15 oxidative stress proteins differed and this was not seen in controls. An increase (P = 0.016) from baseline (73.4 ng/mL) to 24 hours after hypoglycemia (91.7 ng/mL) was seen for urinary isoprostanes. Hypoglycemia resulted in inflammatory and oxidative stress markers being elevated in T2DM subjects but not controls 24-hours after the event.</p><p dir="ltr">Author Correction: Effect of induced hypoglycemia on inflammation and oxidative stress in type 2 diabetes and control subjects: <a href="https://dx.doi.org/10.1038/s41598-020-66189-1" target="_blank">https://dx.doi.org/10.1038/s41598-020-66189-1</a>, published online 19 June 2020.</p><h2>Other Information</h2><p dir="ltr">Published in: Scientific Reports<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="http://dx.doi.org/10.1038/s41598-020-61531-z" target="_blank">http://dx.doi.org/10.1038/s41598-020-61531-z</a></p>2020-03-16T06:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1038/s41598-020-61531-zhttps://figshare.com/articles/journal_contribution/Effect_of_induced_hypoglycemia_on_inflammation_and_oxidative_stress_in_type_2_diabetes_and_control_subjects/21598155CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/215981552020-03-16T06:00:00Z |
| spellingShingle | Effect of induced hypoglycemia on inflammation and oxidative stress in type 2 diabetes and control subjects Hassan Kahal (9999461) Biomedical and clinical sciences Clinical sciences Diabetes complications Type 2 diabetes Inflammation Oxidative Stress |
| status_str | publishedVersion |
| title | Effect of induced hypoglycemia on inflammation and oxidative stress in type 2 diabetes and control subjects |
| title_full | Effect of induced hypoglycemia on inflammation and oxidative stress in type 2 diabetes and control subjects |
| title_fullStr | Effect of induced hypoglycemia on inflammation and oxidative stress in type 2 diabetes and control subjects |
| title_full_unstemmed | Effect of induced hypoglycemia on inflammation and oxidative stress in type 2 diabetes and control subjects |
| title_short | Effect of induced hypoglycemia on inflammation and oxidative stress in type 2 diabetes and control subjects |
| title_sort | Effect of induced hypoglycemia on inflammation and oxidative stress in type 2 diabetes and control subjects |
| topic | Biomedical and clinical sciences Clinical sciences Diabetes complications Type 2 diabetes Inflammation Oxidative Stress |