Clinical outcomes, molecular epidemiology and resistance mechanisms of multidrug-resistant Pseudomonas aeruginosa isolated from bloodstream infections from Qatar
<h3>Background</h3><p dir="ltr">Bloodstream infections (BSIs) caused by multidrug-resistant (MDR)-Pseudomonas aeruginosa are associated with poor clinical outcomes, at least partly due to delayed appropriate antimicrobial therapy. The characteristics of MDR-P. aeruginosa...
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2021
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| _version_ | 1864513512252899328 |
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| author | Mazen A. Sid Ahmed (18810337) |
| author2 | Jemal M. Hamid (11819153) Ahmed A. Husain (11819156) Hamad Abdel Hadi (11819159) Sini Skariah (130134) Ali A. Sultan (7409735) Emad Bashir Ibrahim (9590113) Abdul Latif Al Khal (12213473) Bo Soderquist (11819165) Jana Jass (677208) Ali S. Omrani (9590116) |
| author2_role | author author author author author author author author author author |
| author_facet | Mazen A. Sid Ahmed (18810337) Jemal M. Hamid (11819153) Ahmed A. Husain (11819156) Hamad Abdel Hadi (11819159) Sini Skariah (130134) Ali A. Sultan (7409735) Emad Bashir Ibrahim (9590113) Abdul Latif Al Khal (12213473) Bo Soderquist (11819165) Jana Jass (677208) Ali S. Omrani (9590116) |
| author_role | author |
| dc.creator.none.fl_str_mv | Mazen A. Sid Ahmed (18810337) Jemal M. Hamid (11819153) Ahmed A. Husain (11819156) Hamad Abdel Hadi (11819159) Sini Skariah (130134) Ali A. Sultan (7409735) Emad Bashir Ibrahim (9590113) Abdul Latif Al Khal (12213473) Bo Soderquist (11819165) Jana Jass (677208) Ali S. Omrani (9590116) |
| dc.date.none.fl_str_mv | 2021-12-09T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1080/07853890.2021.2012588 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Clinical_outcomes_molecular_epidemiology_and_resistance_mechanisms_of_multidrug-resistant_Pseudomonas_aeruginosa_isolated_from_bloodstream_infections_from_Qatar/26114401 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Cardiovascular medicine and haematology Clinical sciences Medical microbiology Bacteraemia MDR Pseudomonas aerugi- nosa Qatar |
| dc.title.none.fl_str_mv | Clinical outcomes, molecular epidemiology and resistance mechanisms of multidrug-resistant Pseudomonas aeruginosa isolated from bloodstream infections from Qatar |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <h3>Background</h3><p dir="ltr">Bloodstream infections (BSIs) caused by multidrug-resistant (MDR)-Pseudomonas aeruginosa are associated with poor clinical outcomes, at least partly due to delayed appropriate antimicrobial therapy. The characteristics of MDR-P. aeruginosa bloodstream isolates have not been evaluated in Qatar. Our study aimed to examine in vitro susceptibility, clinical and molecular characteristics, and mechanisms of resistance of MDR-P. aeruginosa bloodstream isolates from Qatar.</p><h3>Materials and methods</h3><p dir="ltr">We included all MDR-P. aeruginosa isolated from blood cultures taken between October 2014 and September 2017. Blood cultures were processed using BD BACTEC™ FX automated system. BD Phoenix™ was used for identification, Liofilchem® MIC Test Strips for MIC determination. Whole-genome sequencing was performed using the Illumina-HiSeq-2000.</p><h3>Results</h3><p dir="ltr">Out of 362 P. aeruginosa bloodstream isolates, 16 (4.4%) were MDR. The median patient age was 55 years (range 43–81) and all patients presented with septic shock. Most patients received meropenem (12/16) and/or colistin (10/16). Clinical response was achieved in eight patients, and five patients died within 30-days. MDR-P. aeruginosa isolates belonged to 13 different sequence types. All isolates were non-susceptible to cefepime and ciprofloxacin. The most active agents were colistin (16/16) and aztreonam (10/16). Seven isolates produced blaVIM, and four possessed genes encoding extended-spectrum β-lactamases. Aminoglycoside modifying enzymes were present in 15/16, transferable qnr-mediated quinolone resistance gene was detected in 3/16, and the novel ciprofloxacin modifying enzyme CrpP-encoding gene in one isolate.</p><h3>Conclusion</h3><p dir="ltr">MDR-P. aeruginosa BSIs are relatively uncommon in Qatar but are highly resistant, harbour multiple resistance genes, and are commonly associated with unfavourable clinical outcomes. Colistin was the only agent with consistent activity against the study isolates.</p><h2>Other Information</h2><p dir="ltr">Published in: Annals of Medicine<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1080/07853890.2021.2012588" target="_blank">https://dx.doi.org/10.1080/07853890.2021.2012588</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_fb886db7a8df731a79036226548aec05 |
| identifier_str_mv | 10.1080/07853890.2021.2012588 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/26114401 |
| publishDate | 2021 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Clinical outcomes, molecular epidemiology and resistance mechanisms of multidrug-resistant Pseudomonas aeruginosa isolated from bloodstream infections from QatarMazen A. Sid Ahmed (18810337)Jemal M. Hamid (11819153)Ahmed A. Husain (11819156)Hamad Abdel Hadi (11819159)Sini Skariah (130134)Ali A. Sultan (7409735)Emad Bashir Ibrahim (9590113)Abdul Latif Al Khal (12213473)Bo Soderquist (11819165)Jana Jass (677208)Ali S. Omrani (9590116)Biomedical and clinical sciencesCardiovascular medicine and haematologyClinical sciencesMedical microbiologyBacteraemiaMDRPseudomonas aerugi- nosaQatar<h3>Background</h3><p dir="ltr">Bloodstream infections (BSIs) caused by multidrug-resistant (MDR)-Pseudomonas aeruginosa are associated with poor clinical outcomes, at least partly due to delayed appropriate antimicrobial therapy. The characteristics of MDR-P. aeruginosa bloodstream isolates have not been evaluated in Qatar. Our study aimed to examine in vitro susceptibility, clinical and molecular characteristics, and mechanisms of resistance of MDR-P. aeruginosa bloodstream isolates from Qatar.</p><h3>Materials and methods</h3><p dir="ltr">We included all MDR-P. aeruginosa isolated from blood cultures taken between October 2014 and September 2017. Blood cultures were processed using BD BACTEC™ FX automated system. BD Phoenix™ was used for identification, Liofilchem® MIC Test Strips for MIC determination. Whole-genome sequencing was performed using the Illumina-HiSeq-2000.</p><h3>Results</h3><p dir="ltr">Out of 362 P. aeruginosa bloodstream isolates, 16 (4.4%) were MDR. The median patient age was 55 years (range 43–81) and all patients presented with septic shock. Most patients received meropenem (12/16) and/or colistin (10/16). Clinical response was achieved in eight patients, and five patients died within 30-days. MDR-P. aeruginosa isolates belonged to 13 different sequence types. All isolates were non-susceptible to cefepime and ciprofloxacin. The most active agents were colistin (16/16) and aztreonam (10/16). Seven isolates produced blaVIM, and four possessed genes encoding extended-spectrum β-lactamases. Aminoglycoside modifying enzymes were present in 15/16, transferable qnr-mediated quinolone resistance gene was detected in 3/16, and the novel ciprofloxacin modifying enzyme CrpP-encoding gene in one isolate.</p><h3>Conclusion</h3><p dir="ltr">MDR-P. aeruginosa BSIs are relatively uncommon in Qatar but are highly resistant, harbour multiple resistance genes, and are commonly associated with unfavourable clinical outcomes. Colistin was the only agent with consistent activity against the study isolates.</p><h2>Other Information</h2><p dir="ltr">Published in: Annals of Medicine<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1080/07853890.2021.2012588" target="_blank">https://dx.doi.org/10.1080/07853890.2021.2012588</a></p>2021-12-09T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1080/07853890.2021.2012588https://figshare.com/articles/journal_contribution/Clinical_outcomes_molecular_epidemiology_and_resistance_mechanisms_of_multidrug-resistant_Pseudomonas_aeruginosa_isolated_from_bloodstream_infections_from_Qatar/26114401CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/261144012021-12-09T03:00:00Z |
| spellingShingle | Clinical outcomes, molecular epidemiology and resistance mechanisms of multidrug-resistant Pseudomonas aeruginosa isolated from bloodstream infections from Qatar Mazen A. Sid Ahmed (18810337) Biomedical and clinical sciences Cardiovascular medicine and haematology Clinical sciences Medical microbiology Bacteraemia MDR Pseudomonas aerugi- nosa Qatar |
| status_str | publishedVersion |
| title | Clinical outcomes, molecular epidemiology and resistance mechanisms of multidrug-resistant Pseudomonas aeruginosa isolated from bloodstream infections from Qatar |
| title_full | Clinical outcomes, molecular epidemiology and resistance mechanisms of multidrug-resistant Pseudomonas aeruginosa isolated from bloodstream infections from Qatar |
| title_fullStr | Clinical outcomes, molecular epidemiology and resistance mechanisms of multidrug-resistant Pseudomonas aeruginosa isolated from bloodstream infections from Qatar |
| title_full_unstemmed | Clinical outcomes, molecular epidemiology and resistance mechanisms of multidrug-resistant Pseudomonas aeruginosa isolated from bloodstream infections from Qatar |
| title_short | Clinical outcomes, molecular epidemiology and resistance mechanisms of multidrug-resistant Pseudomonas aeruginosa isolated from bloodstream infections from Qatar |
| title_sort | Clinical outcomes, molecular epidemiology and resistance mechanisms of multidrug-resistant Pseudomonas aeruginosa isolated from bloodstream infections from Qatar |
| topic | Biomedical and clinical sciences Cardiovascular medicine and haematology Clinical sciences Medical microbiology Bacteraemia MDR Pseudomonas aerugi- nosa Qatar |